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ID   PSV1       preliminary; circular DNA; SYN; 7576 BP.
XX
AC   IG9857;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSV1 - complete, SV40 early promoter.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSV1 from pBR322 & SV40 early genes
RC   pAS from pSV1
RC   pEMP from pSV1 & SV40 T antigen
RC   HS0, HS1, HS2, HS3, HS4, HS6 from pSV1 & pEMP
RC   pHS102 from pBR322 & SV40
RA   Benoist C., Chambon P.;
RT   "Deletions covering the putative promoter region of early mRNAs of
RT   simian virus 40 do not abolish T-antigen expression";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:3865-3869(1980).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSV1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 EcoRI 4361bp 4360..4360, ori/amp
FT                   DNA polymerase I
FT                   2. SV40 BamHI-HpaI 3211bp 2534..5243..502,
FT                   \ early region/0.73 to 0.15
FT                   DNA polymerase I:DNA polymerase I
FT                   -> pSV1 7572bp"
FT   -               1..4363
FT                   /note="pBR322 1..4361..2 4363bp
FT                   EcoRI = G^AATT C
FT                   BamHI =      G^GATCC"
FT   -               4364..7574
FT                   /note="SV40 2534..5243..501 3211bp
FT                   HpaI = GTT^AAC
FT                   EcoRI =  G^AATTC"
FT   -               7575..7576
FT                   /note="pBR322 4360..4361 2bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7576 BP; 1914 A; 1923 C; 1692 G; 2047 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attgatccag acatgataag
     atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcatgaac agactgtgag
     gactgagggg cctgaaatga gccttgggac tgtgaatcaa tgcctgtttc atgccctgag
     tcttccatgt tcttctcccc accatcttca tttttatcag cattttcctg gctgtcttca
     tcatcatcat cactgtttct tagccaatct aaaactccaa ttcccatagc cacattaaac
     ttcatttttt gatacactga caaactaaac tctttgtcca atctctcttt ccactccaca
     attctgctct gaatactttg agcaaactca gccacaggtc tgtaccaaat taacataaga
     agcaaagcaa tgccactttg aattattctc ttttctaaca aaaactcact gcgttccagg
     caatgcttta aataatcttt gggcctaaaa tctatttgtt ttacaaatct ggcctgcagt
     gttttaggca cactgtactc attcatggtg actattccag ggggaaatat ttgagttctt
     ttatttaggt gtttcttttc taagtttacc ttaacactgc catccaaata atcccttaaa
     ttgtccaggt tattaattcc ctgacctgaa ggcaaatctc tggactcccc tccagtgccc
     tttacatcct caaaaactac taaaaactgg tcaatagcta ctcctagctc aaagttcagc
     ctgtccaagg gcaaattaac atttaaagct ttccccccac ataattcaag caaagcagct
     gctaatgtag ttttaccact atcaattggt cctttaaaca gccagtatct ttttttagga
     atgttgtaca ccatgcattt taaaaagtca tacaccactg aatccatttt gggcaacaaa
     cagtgtagcc aagcaactcc agccatccat tcttctatgt cagcagagcc tgtagaacca
     aacattatat ccatcctatc caaaagatca ttaaatctgt ttgttaacat ttgttctcta
     gttaattgta ggctatcaac ccgcttttta gctaaaacag tatcaacagc ctgttggcat
     atggtttttt ggtttttgct gtcagcaaat atagcagcat ttgcataatg cttttcatgg
     tacttatagt ggctgggctg ttctttttta atacatttta aacacatttc aaaactgtac
     tgaaattcca agtacatccc aagcaataac aacacatcat cacattttgt ttccattgca
     tactctgtta caagcttcca ggacacttgt ttagtttcct ctgcttcttc tggattaaaa
     tcatgctcct ttaacccacc tggcaaactt tcctcaataa cagaaaatgg atctctagtc
     aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa gctaaaggta
     cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg agtaagaaaa
     aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga atatttttcc
     ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc aaagcaagca
     agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga aggaaagtcc
     ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga gtcagcagta
     gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc attaaaggca
     ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac acaaacaatt
     agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt agagctttaa
     atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc cttcacaaag
     atcaagtcca aaccacattc taaagcaatc gaagcagtag caatcaaccc acacaagtgg
     atctttcctg tataattttc tattttcatg cttcatcctc agtaagcaca gcaagcatat
     gcagttagca gacattttct ttgcacactc aggccattgt ttgcagtaca ttgcatcaac
     accaggattt aaggaagaag caaatacctc agttgcatcc cagaagcctc caaagtcagg
     ttgatgagca tattttactc catcttccat tttcttgtac agagtattca ttttcttcat
     tttttcttca tctcctcctt tatcaggatg aaactccttg cattttttta aatatgcctt
     tctcatcaga ggaatattcc cccaggcact cctttcaaga cctagaaggt ccattagctg
     caaagattcc tctctgttta aaactttatc catctttgca aagctttttg caaaagccta
     ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
     cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     accctaactg acacacattc cacagctggt tctttccgcc tcagaaggta cctaaccaag
     ttcctctttc agaggttatt tcaggccatg gtgctgcgcc ggctgtcacg ccaggcctcc
     gttaaggttc gtaggtcatg gactgaaagt aaaaaaacag ctcaacgcct ttttgtgttt
     gttttagagc ttttgctgca attttgtgaa ggggaagata ctgttgacgg gaaacgcaaa
     aaaccagaaa ggttaa
//