back Return to this vector's summary.
ID   PSV1CAT    preliminary; circular DNA; SYN; 4756 BP.
XX
AC   IG7039;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pSV1-cat - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tn9 from pBR322 & Tn9]
RC   pSV2-catr, pSV2-cats or pSV2-cat from pBR322-Tn9 & pSV2 & oligo
RC   pSV(Sph)-cat from pSV2-cat
RC   pSV1-cat from pSV2-cat, SV40 small T antigen splice site/polyA
RC   pSV0-cat from pSV2-cat & linker
RC   [pCatB' from SV40 & cat gene]
RA   Gorman C.M., Moffat L.F., Howard B.H.;
RT   "Recombinant genomes which express chloramphenicol acetyltransferase
RT   in mammalian cells";
RL   Mol. Cell. Biol. 2:1044-1051(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Vector for the expression of chloramphenicol acetyltransferase in
CC   eukaryotic cell lines under the control of an inserted promoter
CC   containing DNA fragment.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSV1-cat)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSV2-cat)
CC   BR (pSV0-cat)
CC   OF (pOEV)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2-cat remove SphI-AccI 247bp 258..505,
FT                   \ SV40 early rpt/may be 203..505/4756bp
FT                   Klenow:Klenow
FT                   -> pSV1-cat 4756bp"
FT   -               1..257
FT                   /note="pSV2-cat 1..257 257bp
FT                   SphI = GCATG^C
FT                   AccI =    GT^MKAC"
FT   -               258..4756
FT                   /note="pSV2-cat 505..5003 4499bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
XX
SQ   Sequence 4756 BP; 1330 A; 1056 C; 1080 G; 1290 T; 0 other;
     ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggttgctg
     actaattgag atgcatgata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     aggcgtatca cgaggccctt tcgtcttcaa gaattccttt gcctaattta aatgaggact
     taacctgtgg aaatattttg atgtgggaag ctgttactgt taaaactgag gttattgggg
     taactgctat gttaaacttg cattcaggga cacaaaaaac tcatgaaaat ggtgctggaa
     aacccattca agggtcaaat tttcattttt ttgctgttgg tggggaacct ttggagctgc
     agggtgtgtt agcaaactac aggaccaaat atcctgctca aactgtaacc ccaaaaaatg
     ctacagttga cagtcagcag atgaacactg accacaaggc tgttttggat aaggataatg
     cttatccagt ggagtgctgg gttcctgatc caagtaaaaa tgaaaacact agatattttg
     gaacctacac aggtggggaa aatgtgcctc ctgttttgca cattactaac acagcaacca
     cagtgcttct tgatgagcag ggtgttgggc ccttgtgcaa agctgacagc ttgtatgttt
     ctgctgttga catttgtggg ctgtttacca acacttctgg aacacagcag tggaagggac
     ttcccagata ttttaaaatt acccttagaa agcggtctgt gaaaaacccc tacccaattt
     cctttttgtt aagtgaccta attaacagga ggacacagag ggtggatggg cagcctatga
     ttggaatgtc ctctcaagta gaggaggtta gggtttatga ggacacagag gagcttcctg
     gggatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
     atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
     gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
     agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
     atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
     aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
     aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
     gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
     attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
     acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
     agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
     cttcacaaag atccggcgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacagac
     ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     tgaaaatctc gccaag
//