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ID   PSV2       preliminary; circular DNA; SYN; 4125 BP.
XX
AC   IG9904;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Mammal/E. coli phagemid vector pSV2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pSKS105 & linker
RC   pFRTbetaGAL from plasmid & pSV2
RC   plasmid2 from pUC19 & FRT gene & pMC1neo-polyA
RC   pNEObetaGAL from pFRTbetaGAL & plasmid2
RC   pOG28 from pCDM8 & mouse DHFR gene
RC   pOG44 from pCDM8 & pMLSIScat & yeast FLP recombinase & yeast 2 micron
RC   pOG45 from pNEObetaGAL & pUC19
RC   pOG45A from pOG45
RA   O'Gorman S., Fox D.T., Wahl G.W.;
RT   "Recombinase-mediated gene activation and site-specific integration
RT   in mammalian cells";
RL   Science 251:1351-1355(1991).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSV2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mammalian tissue culture cells)
CC   CP ()
CC   FN (gene targeting)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. SV40 StuI-PvuII 322bp 5194..5243..273,
FT                   \ early promoter/HindIII-PvuII 344bp 5172..5243..273
FT                   2. pBR322 3045bp 324..3369, ori/amp gene
FT                   3. SV40 BamHI-MboI 237bp 2534..2771,
FT                   \ small t antigen [3370..3606]
FT                   4. SV40 MboI-? 521bp 2771..3292, polyA [3606..4127]
FT                   -> pSV2 4125bp"
FT   -               1..322
FT                   /note="SV40 5194..5243..272 322bp
FT                   PvuII = CAG^CTG
FT                   \           tacttg..."
FT   -               323..3367
FT                   /note="pBR322 324..3368 3045bp
FT                   \ ...ctcccc
FT                   BamHI =   G^GATCC"
FT   -               3368..3604
FT                   /note="SV40 2534..2770 237bp
FT                   MboI = Sau3AI = ^GATC"
FT   -               3605..4125
FT                   /note="SV40 2771..3291 521bp
FT                   StuI = AGG^CCT"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
XX
SQ   Sequence 4125 BP; 948 A; 1109 C; 1056 G; 1012 T; 0 other;
     ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
     aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     ctaactgaca cacattccac agtacttgga gccactatcg actacgcgat catggcgacc
     acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg
     cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg
     ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
     gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
     gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
     aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
     gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
     cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
     gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
     tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca
     aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc
     tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg
     accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     actccccgat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     ttatgatcat gaacagactg tgaggactga ggggcctgaa atgagccttg ggactgtgaa
     tcaatgcctg tttcatgccc tgagtcttcc atgttcttct ccccaccatc ttcattttta
     tcagcatttt cctggctgtc ttcatcatca tcatcactgt ttcttagcca atctaaaact
     ccaattccca tagccacatt aaacttcatt ttttgataca ctgacaaact aaactctttg
     tccaatctct ctttccactc cacaattctg ctctgaatac tttgagcaaa ctcagccaca
     ggtctgtacc aaattaacat aagaagcaaa gcaatgccac tttgaattat tctcttttct
     aacaaaaact cactgcgttc caggcaatgc tttaaataat ctttgggcct aaaatctatt
     tgttttacaa atctggcctg cagtgtttta ggcacactgt actcattcat ggtgactatt
     ccagggggaa atatttgagt tcttttattt aggtgtttct tttct
//