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ID   PSV2CAT    preliminary; circular DNA; SYN; 5003 BP.
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AC   M77788; ATCC37155;
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DT   23-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSV2cat - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5003
RC   pSV2-cat
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pSV2 from pBR322 & SV40
RC   pSV2-cat from pSV2
RC   pRSVneo, pRSVcat, pRSVgpt, pRSV/betaglobin from RSV
RA   Gorman C.M.;
RT   "High efficiency transfer into mammalian cells";
RL   DNA Cloning 2:143-190(1985).
RL   Glover D.M., ed., IRL Press, Oxford.
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RN   [3]
RC   SVGT12-betaG, SVGT14-betaG from SV40 & rabbit beta-globin
RC   pSVGT10-betaG from pBR322 & SVGT10-betaG
RC   pSVGT10*-betaG from pBR322 & SVGT10*-betaG
RC   pSVGT12-betaG from pBR322 & SVGT12-betaG
RC   pSVGT14-betaG from pBR322 & SVGT14-betaG
RA   Southern P.J., Howard B.H., Berg P.;
RT   "Construction and characterization of SV40 recombinants with
RT   beta-globin cDNA substitutions in their early regions";
RL   J. Mol. Appl. Genet. 1:177-190(1981).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
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CC   ATCC size is 5100 bp.
CC   Restriction digests of the vector give the following sizes (kb):
CC   HindIII--5.0; BamHI--5.0; BamHI/HindIII--3.5, 1.6; AccI--5.0.
CC   (ATCC staff)
CC   This vector expresses chloramphenicol acetyltransferase in eukaryotic
CC   cell lines under the control of the simian virus 40 early promoter.
CC   (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSV2cat)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)(vertebrate cells)(monkey)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Rhesus macaque polyomavirus)
CC   BR ()
CC   OF (pSV2catSVgpt)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 ClaI 4361bp 25..25
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pBR322-Tn9 5134bp
FT                   1. SV40 StuI-PvuII 323bp 5194..5243..273,
FT                   \ early promoter/HindIII-PvuII 344bp 5172..5243..273
FT                   2. pBR322 3045bp 324..3369, ori/amp gene
FT                   3. SV40 BamHI-MboI 237bp 2534..2771,
FT                   \ small t antigen [3370..3606]
FT                   4. SV40 MboI-? 521bp 2771..3292, polyA [3606..4127]
FT                   -> pSV2 4125bp
FT                   1. pSV2 4125bp
FT                   2. pBR322-Tn9 TaqI-TaqI 773bp 215..988,
FT                   \ cat gene/no promoter
FT                   Klenow:Klenow
FT                   HindIII linker 6bp aagctt:BamHI linker 6bp ggatcc,
FT                   \ 785bp 4218..5003
FT                   -> pSV2-cat 5003bp"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   misc_feature    0..0
FT                   /note="SV40"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT HincII"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique AccI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 genes"
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SQ   Sequence 5003 BP; 1377 A; 1128 C; 1159 G; 1339 T; 0 other;
     ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggttgctg
     actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     accctaactg acacacattc cacagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttcctttgcc taatttaaat
     gaggacttaa cctgtggaaa tattttgatg tgggaagctg ttactgttaa aactgaggtt
     attggggtaa ctgctatgtt aaacttgcat tcagggacac aaaaaactca tgaaaatggt
     gctggaaaac ccattcaagg gtcaaatttt catttttttg ctgttggtgg ggaacctttg
     gagctgcagg gtgtgttagc aaactacagg accaaatatc ctgctcaaac tgtaacccca
     aaaaatgcta cagttgacag tcagcagatg aacactgacc acaaggctgt tttggataag
     gataatgctt atccagtgga gtgctgggtt cctgatccaa gtaaaaatga aaacactaga
     tattttggaa cctacacagg tggggaaaat gtgcctcctg ttttgcacat tactaacaca
     gcaaccacag tgcttcttga tgagcagggt gttgggccct tgtgcaaagc tgacagcttg
     tatgtttctg ctgttgacat ttgtgggctg tttaccaaca cttctggaac acagcagtgg
     aagggacttc ccagatattt taaaattacc cttagaaagc ggtctgtgaa aaacccctac
     ccaatttcct ttttgttaag tgacctaatt aacaggagga cacagagggt ggatgggcag
     cctatgattg gaatgtcctc tcaagtagag gaggttaggg tttatgagga cacagaggag
     cttcctgggg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     gattatgatc tctagtcaag gcactataca tcaaatattc cttattaacc cctttacaaa
     ttaaaaagct aaaggtacac aatttttgag catagttatt aatagcagac actctatgcc
     tgtgtggagt aagaaaaaac agtatgttat gattataact gttatgccta cttataaagg
     ttacagaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt
     aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttagca
     attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat
     gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt
     tttcctcatt aaaggcattc caccactgct cccattcatc agttccatag gttggaatct
     aaaatacaca aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat
     ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt
     aaggttcctt cacaaagatc cggcgaattt ctgccattca tccgcttatt atcacttatt
     caggcgtagc accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc
     cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     tagctcctga aaatctcgcc aag
//