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ID   PSV3NEO    preliminary; circular DNA; SYN; 8571 BP.
XX
AC   ATCC37150;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pSV3-neo - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR-neo from pRZ112 & pBR322
RC   pSV2-neo from pBR-neo & pSV2-betaG
RC   pSV3-neo from pSV2-neo
RC   pSV5-neo from pSV2-neo
RC   [SVGT10-betaG from SV40 & rabbit beta-globin gene]
RC   [SVGT10*-betaG from SVGT10-betaG]
RC   [pSV2 from pBR322 & SV40 early]
RC   [pSV2-betaG from pSV2 & rabbit beta-globin gene]
RC   [pSV2-neo-SVgpt from pSV2-neo & pSV2-gpt]
RA   Southern P.J., Berg P.;
RT   "Transformation of mammalian cells to antibiotic resistance with a
RT   bacterial gene under control of the SV40 early region promoter";
RL   J. Mol. Appl. Genet. 1:327-341(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Provides dominant selectable marker for resistance to antibiotic G418
CC   in mammalian cell lines. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSV3-neo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSV2-neo)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2-neo BamHI 5729bp 752..752
FT                   2. SV40 Sau3AI/MboI-Sau3AI/MboI 2842bp 874..3716,
FT                   \ early region/SV40 ori
FT                   -> pSV3-neo 8571bp"
FT   -               1..751
FT                   /note="pSV2-neo 1..751 751bp
FT                   BamHI =        G^GATCC
FT                   MboI = Sau3AI = ^GATC"
FT   -               752..3593
FT                   /note="SV40 874..3715 2842bp
FT                   MboI = Sau3AI = ^GATC
FT                   BamHI =        G^GATCC"
FT   -               3594..8571
FT                   /note="pSV2-neo 752..5729 4978bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT vertebrate G418 resistance gene (G418)"
XX
SQ   Sequence 8571 BP; 2328 A; 2001 C; 1981 G; 2261 T; 0 other;
     aattcctttg cctaatttaa atgaggactt aacctgtgga aatattttga tgtgggaagc
     tgttactgtt aaaactgagg ttattggggt aactgctatg ttaaacttgc attcagggac
     acaaaaaact catgaaaatg gtgctggaaa acccattcaa gggtcaaatt ttcatttttt
     tgctgttggt ggggaacctt tggagctgca gggtgtgtta gcaaactaca ggaccaaata
     tcctgctcaa actgtaaccc caaaaaatgc tacagttgac agtcagcaga tgaacactga
     ccacaaggct gttttggata aggataatgc ttatccagtg gagtgctggg ttcctgatcc
     aagtaaaaat gaaaacacta gatattttgg aacctacaca ggtggggaaa atgtgcctcc
     tgttttgcac attactaaca cagcaaccac agtgcttctt gatgagcagg gtgttgggcc
     cttgtgcaaa gctgacagct tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa
     cacttctgga acacagcagt ggaagggact tcccagatat tttaaaatta cccttagaaa
     gcggtctgtg aaaaacccct acccaatttc ctttttgtta agtgacctaa ttaacaggag
     gacacagagg gtggatgggc agcctatgat tggaatgtcc tctcaagtag aggaggttag
     ggtttatgag gacacagagg agcttcctgg ggatcacaaa gtttctactg ttggtttata
     tcaacaacca ggaatggctg tagatttgta taggccagat gattactatg atattttatt
     tcctggagta caaacctttg ttcacagtgt tcagtatctt gaccccagac attggggtcc
     aacacttttt aatgccattt ctcaagcttt ttggcgtgta atacaaaatg acattcctag
     gctcacctca caggagcttg aaagaagaac ccaaagatat ttaagggaca gtttggcaag
     gtttttagag gaaactactt ggacagtaat taatgctcct gttaattggt ataactcttt
     acaagattac tactctactt tgtctcccat taggcctaca atggtgagac aagtagccaa
     cagggaaggg ttgcaaatat catttgggca cacctatgat aatattgatg aagcagacag
     tattcagcaa gtaactgaga ggtgggaagc tcaaagccaa agtcctaatg tgcagtcagg
     tgaatttatt gaaaaatttg aggctcctgg tggtgcaaat caaagaactg ctcctcagtg
     gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa aagcttatga
     agatggcccc aacaaaaaga aaaggaagtt gtccaggggc agctcccaaa aaaccaaagg
     aaccagtgca agtgccaaag ctcgtcataa aaggaggaat agaagttcta ggagttaaaa
     ctggagtaga cagcttcact gaggtggagt gctttttaaa tcctcaaatg ggcaatcctg
     atgaacatca aaaaggctta agtaaaagct tagcagctga aaaacagttt acagatgact
     ctccagacaa agaacaactg ccttgctaca gtgtggctag aattcctttg cctaatttaa
     atgaggactt aacctgtgga aatattttga tgtgggaagc tgttactgtt aaaactgagg
     ttattggggt aactgctatg ttaaacttgc attcagggac acaaaaaact catgaaaatg
     gtgctggaaa acccattcaa gggtcaaatt ttcatttttt tgctgttggt ggggaacctt
     tggagctgca gggtgtgtta gcaaactaca ggaccaaata tcctgctcaa actgtaaccc
     caaaaaatgc tacagttgac agtcagcaga tgaacactga ccacaaggct gttttggata
     aggataatgc ttatccagtg gagtgctggg ttcctgatcc aagtaaaaat gaaaacacta
     gatattttgg aacctacaca ggtggggaaa atgtgcctcc tgttttgcac attactaaca
     cagcaaccac agtgcttctt gatgagcagg gtgttgggcc cttgtgcaaa gctgacagct
     tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa cacttctgga acacagcagt
     ggaagggact tcccagatat tttaaaatta cccttagaaa gcggtctgtg aaaaacccct
     acccaatttc ctttttgtta agtgacctaa ttaacaggag gacacagagg gtggatgggc
     agcctatgat tggaatgtcc tctcaagtag aggaggttag ggtttatgag gacacagagg
     agcttcctgg ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     ctgattatga tcatgaacag actgtgagga ctgaggggcc tgaaatgagc cttgggactg
     tgaatcaatg cctgtttcat gccctgagtc ttccatgttc ttctccccac catcttcatt
     tttatcagca ttttcctggc tgtcttcatc atcatcatca ctgtttctta gccaatctaa
     aactccaatt cccatagcca cattaaactt cattttttga tacactgaca aactaaactc
     tttgtccaat ctctctttcc actccacaat tctgctctga atactttgag caaactcagc
     cacaggtctg taccaaatta acataagaag caaagcaatg ccactttgaa ttattctctt
     ttctaacaaa aactcactgc gttccaggca atgctttaaa taatctttgg gcctaaaatc
     tatttgtttt acaaatctgg cctgcagtgt tttaggcaca ctgtactcat tcatggtgac
     tattccaggg ggaaatattt gagttctttt atttaggtgt ttcttttcta agtttacctt
     aacactgcca tccaaataat cccttaaatt gtccaggtta ttaattccct gacctgaagg
     caaatctctg gactcccctc cagtgccctt tacatcctca aaaactacta aaaactggtc
     aatagctact cctagctcaa agttcagcct gtccaagggc aaattaacat ttaaagcttt
     ccccccacat aattcaagca aagcagctgc taatgtagtt ttaccactat caattggtcc
     tttaaacagc cagtatcttt ttttaggaat gttgtacacc atgcatttta aaaagtcata
     caccactgaa tccattttgg gcaacaaaca gtgtagccaa gcaactccag ccatccattc
     ttctatgtca gcagagcctg tagaaccaaa cattatatcc atcctatcca aaagatccag
     acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatctctagt
     caaggcacta tacatcaaat attccttatt aaccccttta caaattaaaa agctaaaggt
     acacaatttt tgagcatagt tattaatagc agacactcta tgcctgtgtg gagtaagaaa
     aaacagtatg ttatgattat aactgttatg cctacttata aaggttacag aatatttttc
     cataattttc ttgtatagca gtgcagcttt ttcctttgtg gtgtaaatag caaagcaagc
     aagagttcta ttactaaaca cagcatgact caaaaaactt agcaattctg aaggaaagtc
     cttggggtct tctacctttc tcttcttttt tggaggagta gaatgttgag agtcagcagt
     agcctcatca tcactagatg gcatttcttc tgagcaaaac aggttttcct cattaaaggc
     attccaccac tgctcccatt catcagttcc ataggttgga atctaaaata cacaaacaat
     tagaatcagt agtttaacac attatacact taaaaatttt atatttacct tagagcttta
     aatctctgta ggtagtttgt ccaattatgt cacaccacag aagtaaggtt ccttcacaaa
     gatccgggac caaagcggcc atcgtgcctc cccactcctg cagttcgggg gcatggatgc
     gcggatagcc gctgctggtt tcctggatgc cgacggattt gcactgccgg tagaactccg
     cgaggtcgtc cagcctcagg cagcagctga accaactcgc gaggggatcg agcccggggt
     gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga
     aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg
     gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc
     gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc gtgaagcttt
     ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc
     gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     ggggactttc cacaccctaa ctgacacaca ttccacagct gcctcgcgcg tttcggtgat
     gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa g
//