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ID   PSV53      preliminary; circular DNA; SYN; 5406 BP.
XX
AC   IG9953;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSV53 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSV53 from pBR322 & SV40
RA   Lai C.J.;
RT   ;
RL   Unpublished (1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSV53)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(monkey COS cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 HindIII-BamHI 1040bp 1494..2534, late region
FT                   BamHI linker 6bp ggatcc:
FT                   -> pSV53 5401bp"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC
FT                   \         gatcc"
FT   -               376..380
FT                   /note="gatcc 5bp
FT                   \     gatcc
FT                   HindIII = A^AGCTT"
FT   -               381..1420
FT                   /note="SV40 1494..2533 1040bp
FT                   BamHI = G^GATCC"
FT   -               1421..5406
FT                   /note="pBR322 376..4361 3986bp"
FT   promoter        0..0
FT                   /note="PRO SV40 late genes"
FT   intron          0..0
FT                   /note="SV40 VP1 intron"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 late genes"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5406 BP; 1309 A; 1408 C; 1386 G; 1303 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc agcttatgaa gatggcccca acaaaaagaa aaggaagttg
     tccaggggca gctcccaaaa aaccaaagga accagtgcaa gtgccaaagc tcgtcataaa
     aggaggaata gaagttctag gagttaaaac tggagtagac agcttcactg aggtggagtg
     ctttttaaat cctcaaatgg gcaatcctga tgaacatcaa aaaggcttaa gtaaaagctt
     agcagctgaa aaacagttta cagatgactc tccagacaaa gaacaactgc cttgctacag
     tgtggctaga attcctttgc ctaatttaaa tgaggactta acctgtggaa atattttgat
     gtgggaagct gttactgtta aaactgaggt tattggggta actgctatgt taaacttgca
     ttcagggaca caaaaaactc atgaaaatgg tgctggaaaa cccattcaag ggtcaaattt
     tcattttttt gctgttggtg gggaaccttt ggagctgcag ggtgtgttag caaactacag
     gaccaaatat cctgctcaaa ctgtaacccc aaaaaatgct acagttgaca gtcagcagat
     gaacactgac cacaaggctg ttttggataa ggataatgct tatccagtgg agtgctgggt
     tcctgatcca agtaaaaatg aaaacactag atattttgga acctacacag gtggggaaaa
     tgtgcctcct gttttgcaca ttactaacac agcaaccaca gtgcttcttg atgagcaggg
     tgttgggccc ttgtgcaaag ctgacagctt gtatgtttct gctgttgaca tttgtgggct
     gtttaccaac acttctggaa cacagcagtg gaagggactt cccagatatt ttaaaattac
     ccttagaaag cggtctgtga aaaaccccta cccaatttcc tttttgttaa gtgacctaat
     taacaggagg acacagaggg tggatgggca gcctatgatt ggaatgtcct ctcaagtaga
     ggaggttagg gtttatgagg acacagagga gcttcctggg gatcctctac gccggacgca
     tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca
     ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta
     tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc
     ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt
     cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
     ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
     gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
     cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
     cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
     tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac
     gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
     gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
     acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
     aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
     cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
     tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
     taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
     ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
     cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
     ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc
     gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     caagaa
//