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ID   PSV5NEO    preliminary; circular DNA; SYN; 9475 BP.
XX
AC   ATCC37151;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pSV5-neo - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR-neo from pRZ112 & pBR322
RC   pSV2-neo from pBR-neo & pSV2-betaG
RC   pSV3-neo from pSV2-neo
RC   pSV5-neo from pSV2-neo
RC   [SVGT10-betaG from SV40 & rabbit beta-globin gene]
RC   [SVGT10*-betaG from SVGT10-betaG]
RC   [pSV2 from pBR322 & SV40 early]
RC   [pSV2-betaG from pSV2 & rabbit beta-globin gene]
RC   [pSV2-neo-SVgpt from pSV2-neo & pSV2-gpt]
RA   Southern P.J., Berg P.;
RT   "Transformation of mammalian cells to antibiotic resistance with a
RT   bacterial gene under control of the SV40 early region promoter";
RL   J. Mol. Appl. Genet. 1:327-341(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Provides dominant selectable marker for resistance to antibiotic G418
CC   in mammalian cell lines. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSV5-neo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSV2-neo)(polyoma)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2-neo BamHI 5729bp 752..752
FT                   2. polyoma BglII-BglII 3746bp 3..3749, early reg/ori
FT                   \ no BamHI [4754bp total]
FT                   \ BglII = 3 1975 3749 4244
FT                   \ MboI = 3 343 504 742 904 932 1004 1975 2383 2684
FT                   \ 2695 3749 4244 4676
FT                   -> pSV5-neo 9475bp"
FT   -               1..751
FT                   /note="pSV2-neo 1..751 751bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               752..4497
FT                   /note="polyoma 3..3748 3746bp
FT                   BglII = A^GATCT
FT                   BamHI = G^GATCC"
FT   -               4498..9475
FT                   /note="pSV2-neo 752..5729 4978bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli gpt+ gene"
FT   CDS             0..0
FT                   /note="ANT mammal G418 resistance gene (G418)"
XX
SQ   Sequence 9475 BP; 2606 A; 2153 C; 2182 G; 2534 T; 0 other;
     aattcctttg cctaatttaa atgaggactt aacctgtgga aatattttga tgtgggaagc
     tgttactgtt aaaactgagg ttattggggt aactgctatg ttaaacttgc attcagggac
     acaaaaaact catgaaaatg gtgctggaaa acccattcaa gggtcaaatt ttcatttttt
     tgctgttggt ggggaacctt tggagctgca gggtgtgtta gcaaactaca ggaccaaata
     tcctgctcaa actgtaaccc caaaaaatgc tacagttgac agtcagcaga tgaacactga
     ccacaaggct gttttggata aggataatgc ttatccagtg gagtgctggg ttcctgatcc
     aagtaaaaat gaaaacacta gatattttgg aacctacaca ggtggggaaa atgtgcctcc
     tgttttgcac attactaaca cagcaaccac agtgcttctt gatgagcagg gtgttgggcc
     cttgtgcaaa gctgacagct tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa
     cacttctgga acacagcagt ggaagggact tcccagatat tttaaaatta cccttagaaa
     gcggtctgtg aaaaacccct acccaatttc ctttttgtta agtgacctaa ttaacaggag
     gacacagagg gtggatgggc agcctatgat tggaatgtcc tctcaagtag aggaggttag
     ggtttatgag gacacagagg agcttcctgg ggatcttgga gagttcctgt ttacatacat
     tgaaccgccc ccattgtctt ggcttcctgc cattgccgtg tccgggcaat cagccattgc
     ggtatccggg caatctgccc ggtcttggca aggcgcccgg gagcacttat gaataacgcc
     cttcctcctc ttaatagcaa taaattcttg agcagcagcc aacacagttt aagcagactc
     atttttttcc aagggtataa gaggctggag gcctttgccc ctaccttcac tgcagaaaga
     gagaggcttt tgagaggctt ttcagaggct ttccgaggct ttaacctttg cctgaaaaaa
     aaacaaaaat ggatcatcag ttaacaagag aagaaagcca aaggcttatg caccttttaa
     aactccctat ggagcaatat gggaatttcc ctttaatgag aaaggctttt ctaagagcct
     gcaaaattgt gcatccagat aagggaggaa gcgatgaatt gtctcaagaa ttgatcagtc
     tctatagaag acttgaagaa tcccttcctt gcctgagtac tcaggatttc attgaaactg
     ataaggtttg tctaattgag aaaattgatt atcttacaga ttggataaac tgcaattttg
     agaattgcaa taaatgcctt tattgccgcc tatggaataa ccataaaagt gatccgcctt
     tcccaaaagt ttggggatat tgcctctgct acaaatgcta tataatttgg tttggcctgg
     aaccatgtca ttttgcgttt caaagttgga tgcagataat tgctttaaca cctttctgtg
     cttaaatatt taaggtaagt tttattttgc agattccttc atatggaacc cctgaatggg
     atgaatggtg gaaagaattt aataaagatt ttgatctctt ttgtaatgaa gcctttgaca
     gatcggatga tgagcaagaa ccacagcccg acgactcagc acccatcatc ctctcgccca
     cctaccccgc cagatcacag gctaccccac ctaaaaaaaa ggcaaaaatg gattctccaa
     atgatatgcc tgctgactta atggaatacc taagctgtgc tatcctttca aataagacat
     taccttgctt tctgatttac accaccttag aaaaagtgga gttgctttat aataaattat
     ctgaggaagt attaagtcct agatttaatc aggtagacca taaatatgga aggaaatacg
     ttccaatttt tatcataact ggaactaaac acagagttag cgcagtgttt aattattgtg
     caacatattg tagtgtttct tttatagttg ttaaaggtgt aattaaggaa tatccattat
     attgtcattt atgtgtagaa ccctattctg tcctgcaaga gagtattgaa gagttaaatt
     cagaattctt tgatgcccca gaggatgccg caaaaaatgt gaattgggta gcaatatctg
     aatatgcgct aaaaatcaat tgtgatgata tttacctatt aatggggttg tataaagagt
     tccaaagtcc agttcccaac tgctcaaaat gcgagaatag aatgcttaca aatcacttta
     aattccacaa ggaacatcat gaaaatgcct tttatttgca gcttgtagaa aaccagaaaa
     ctatatgcca gcaggcggta gatggtgtaa tagcaacaaa gacggtagat atggcacaaa
     tgacaagaaa tgaacagtta gccgctaggt ttgataaatt atttgaaaga ttggaagtaa
     tattgagtgc gcaatcctcc tatacaatta gcatgtttat ggcaggcatt gtttggtttg
     aaaatctttt tccaggccag tcatttaaag acctcctttt ggaactcctt gaatgtatgg
     tttcaaacat ccctaagaga agatattggc tatttaccgg tcctgttaat actggtaaaa
     caaccttagc agcagctgtc ctagatcttg gaggtacgat atatatatcc gattgttacc
     taattgagct ggaagttgcg aatagtcaaa ttcatggttg tgttgaagtg ttggcagaga
     aagtgaaaat aagattgccg cccgggcaag gaataaataa tcttgataat cttagagatc
     atttagatgg agctgtaaag gtgaacttag aaaagaaaca tttgaataag aaaacccaga
     tatttcctcc tggaattgtt acttcaaatg aatatttcat tccctttact ttaagagtca
     gattttgcaa gaaacttgtc tttaaatttt ctaaatacca atatctatca ttgaaaaaga
     ccgaatgtct tggtagatat agaattctac aaaatggatg tacccttctg cttctcctca
     tttatcattg tgatcttgat gactttgctg aaagcataca agggaaggtt agagcctgga
     aagaaagagt taactctgaa atttctgttt caacatattt ggaaatgaga caatgttgtc
     tagagggaag gtattctgtc tgcacgaaat actcaaatgc caacacagca caatgaataa
     agtaaatgtt tattaacact gcttgcatca caacacaata aaagcttgca tgacaaaaaa
     atacaagtgt ttatgcctca ttgctgggaa tatcagggac agtaaccttc ctggccaaat
     tatccggtat ctgatcatgt caggatctcc aggtagacgt tccactccat cataaatcct
     gacctcctca acctggagca tccatgaatt taggggcatc atctcactgg aaaaggtatt
     cagcaatgag ctcacagggt agggatttgc acagcctttt cttaagtgta tgctgaaata
     tctaggcaag ccacgaaggt gcattttgtt attctgctgt gttctgaagc caacaatgtc
     agcagaagca aggtacaagc catctccctt acaaagcggc cccactccat tttcattcaa
     aaggattgtg gtagtagtgt tagtaatttg caggacaggt ggggtttgaa ggccaccagt
     gtaggttcca aagtatcttg tattctcatt tttggcaggg tcggggctcc atgcttcaat
     tgggaacttt ccatcagcgg ttagcttagc ttttaattta gggtcaagga cctgagcagc
     tttaggagct gcttgaaggg cagccacacc agcagggtaa ttgcacctgt agtttttcca
     caaccattgc aaatcaagtg cttcacctcc aacagagaag aaatggaaat ttagaccttg
     aacaggaaag cctgcacctt cattatcata caaccttttt acagcagagt gacaatttgt
     tagagaggaa accccgacaa cctctgtctt acaacttata gcttcccaca taagtattgt
     atcacatgtc atgtcctcat tcaacattgg gagggaaatt ttggccatac tatagcatgg
     cagttcttta atgcggggca tgtcctgcgt gtagtcagct gaaacagtga tattgtcact
     aaagccatag aaatcagaat caacaggctt ccccatgcga ggattgagga aggcctcaat
     ttgggttata ctatcaggac cagttctgac atcaagcact tggattcctc caacaactac
     tgagttttgg gacttctgag ggcttaggcc ttggttttgc gacgttcgct ttttgactgt
     gggagccatc ttcctctgcc tctaattctt ccaaagtatc tttccaactg ggagtgagat
     ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctc
     tagtcaaggc actatacatc aaatattcct tattaacccc tttacaaatt aaaaagctaa
     aggtacacaa tttttgagca tagttattaa tagcagacac tctatgcctg tgtggagtaa
     gaaaaaacag tatgttatga ttataactgt tatgcctact tataaaggtt acagaatatt
     tttccataat tttcttgtat agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc
     aagcaagagt tctattacta aacacagcat gactcaaaaa acttagcaat tctgaaggaa
     agtccttggg gtcttctacc tttctcttct tttttggagg agtagaatgt tgagagtcag
     cagtagcctc atcatcacta gatggcattt cttctgagca aaacaggttt tcctcattaa
     aggcattcca ccactgctcc cattcatcag ttccataggt tggaatctaa aatacacaaa
     caattagaat cagtagttta acacattata cacttaaaaa ttttatattt accttagagc
     tttaaatctc tgtaggtagt ttgtccaatt atgtcacacc acagaagtaa ggttccttca
     caaagatccg ggaccaaagc ggccatcgtg cctccccact cctgcagttc gggggcatgg
     atgcgcggat agccgctgct ggtttcctgg atgccgacgg atttgcactg ccggtagaac
     tccgcgaggt cgtccagcct caggcagcag ctgaaccaac tcgcgagggg atcgagcccg
     gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc
     cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt
     gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga
     actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
     gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
     acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
     agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
     cctcgccgtc gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
     gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
     gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
     gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
     ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
     cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
     cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
     cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
     catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
     caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc ctgcgccatc
     agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
     agggcgcccc agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct
     atcgccatgt aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg
     tccagatagc ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt
     tctacgtgtt ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag
     ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     gcctggggac tttccacacc ctaactgaca cacattccac agctgcctcg cgcgtttcgg
     tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaag
//