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ID   PSVB10     preliminary; circular DNA; SYN; 2678 BP.
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AC   M22836; M22837; M22838; M22839; M22840; M22841; M22842; M22843;
AC   M22844; M22845; M22846;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pSVB10 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSVB10 from pUC8 & pCSV03
RC   pSVB20 from M13mp8 & pSVB10
RC   pSVB25 from M13mp19 & pSVB10
RC   pSVB22 from pGV401 & pSVB20
RC   pSVB21 from pSVB22
RC   pSVB24 from M13mp19 & pSVB22
RC   pSVB23 from pFR109 & pSVB21
RC   pSVB26 from pSVB24
RC   pSVB28 from pSVB23
RC   pSVB30 from pSVB24 & linker
RC   pSVB31 from pSVB25 & linker
RA   Arnold W., Puhler A.;
RT   "A family of high-copy-number plasmid vectors with single end-label
RT   sites for rapid nucleotide sequencing";
RL   Gene 70:171-179(1988).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS. oligonucleotide linker. in GenBank.
CC   NM (pSVB10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)(pBR327)
CC   BR (pSVB20)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR327 AvaI-HindIII 1878bp 1425..3273..30
FT                   Klenow:Klenow
FT                   -> pGV001 1882bp [HindIII, no AvaI]
FT                   1. pGV001 ClaI 1882bp 25..25
FT                   Klenow with CG only
FT                   2. SmaI-BstEII linker 10bp gtgacccggg
FT                   -> pCSV03 1894bp
FT                   1. pUC8 remove EcoRI-HindIII 30bp 231..261,
FT                   \ MCS/2635bp
FT                   2. pCSV03 EcoRI-HindIII, MCS/pBR327 3272..3273..30
FT                   -> pSVB10 2670bp"
FT   -               1..230
FT                   /note="pUC8 1..230 230bp
FT                   EcoRI = G^AATTC"
FT   -               231..258
FT                   /note="pBR327 3272..3273..26 28bp
FT                   ClaI = AT^CG AT
FT                   \            gtgacccggg"
FT   -               259..268
FT                   /note="gtgacccggg 10bp
FT                   \ gtgacccggg
FT                   ClaI =    AT^CGAT"
FT   -               269..273
FT                   /note="pBR327 25..29 5bp
FT                   HindIII = A^AGCTT"
FT   -               274..2678
FT                   /note="pUC8 261..2665 2405bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BstEII-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2678 BP; 658 A; 679 C; 672 G; 669 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattctcatg
     tttgacagct tatcatcggt gacccgggcg ataagcttgg cactggccgt cgttttacaa
     cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     accgagcgca gcgagtcagt gagcgaggaa gcggaaga
//