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ID   PSVBETA    preliminary; circular DNA; SYN; 6894 BP.
XX
AC   IG1119; ATCC77176;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSVbeta - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pADbeta from adenovirus 2 MLP & SV40 splice/polyA & pUC19
RC   pCMVbeta from CMV IEP & SV40 splice/polyA & pUC19
RC   pSVbeta from SV40 early promoter & SV40 splice/polyA & pUC19
RC   pTKbeta from HSV TK gene promoter & SV40 splice/polyA & pUC19
RA   MacGregor G.R., Caskey C.T.;
RT   "Construction of plasmids that express E. coli beta-galactosidase
RT   in mammalian cells";
RL   Nucleic Acids Res. 17:2365-2365(1989).
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RN   [2]
RC   pSV0-cat, pSV2-cat, pA10CAT2, pCAT..., pBLCAT2, pBLCAT3 from cat gene
RC   pSK-SVCAT, pSK-CAT from cat gene
RC   pRSVL-Adelta5', pSV2AL-Adelta5', pT81luc, pSluc2 from luciferase gene
RC   pXP1, pXP2, pOLUC from luciferase gene
RC   pXGH, pTKGH, pOGH from growth hormone gene
RC   pSV2Apap, pSV232Apap, pSV0Apap from alkaline phosphatase gene
RC   pBC12/RSV/SEAP, pBC12/PL/SEAP from secreted alkaline phosphatase
RC   pCMVbeta, pSVbeta, pNASSbeta from beta-galactosidase gene
RA   Alam J., Cook J.L.;
RT   "Reporter genes: application to the study of mammalian gene
RT   transcription";
RL   Anal. Biochem. 188:245-254(1990).
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CC   Recommended ampicillin concentration is 200 ug/ml.
CC   (personal communication)
CC   The order of the major features in this plasmid is: pUC19 - EcoRI -
CC   SV40 enhancer - SV40 early promoter - SV40 late 16S/19S splice
CC   signals - NotI - lacZ - NotI - SV40 late polyadenylation signal -
CC   HindIII - pUC19. [1]
CC   Reporter plasmid permitting visual detection of beta-galactosidase
CC   activity by histochemical staining or fluorometric assay. [1]
CC   Designed to express high levels of beta-galactosidase or an
CC   alternative gene product in COS cells. May be used as a reference
CC   plasmid in transfection experiments or to monitor the effect of
CC   trans-acting factors. [1]
CC   The lacZ gene may be excised by NotI and replaced with alternative
CC   sequences. [1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--7.0; HindIII--7.0; EcoRI/HindIII--4.3, 2.7; NotI--3.6
CC   (doublet); BamHI--3.6, 3.2, 0.2. (ATCC staff)
CC   Medium is -1 TB plus ampicillin (200 ug/ml).
CC   NM (pSVbeta)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(ATCC)
CC   HO (E.coli DH5alpha)(E.coli)(monkey COS-1 or COS-7 cells)
CC   HO (vertebrate cells)
CC   CP ()
CC   FN (expression)(reporter)(promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR (pADbeta)(pNASSbeta)(pCMVbeta)(pTKbeta)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. SV40, splice/polyA
FT                   -> plasmid
FT                   1. plasmid NotI
FT                   2. E. coli NotI-NotI, lacZ gene
FT                   -> plasmid2
FT                   1. plasmid2
FT                   2. SV40, early promoter
FT                   -> pSVbeta 6894bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SstI-BamHI-XbaI-SalI-PstI-SphI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SstI-XbaI-SalI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 enhancer"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    3900..3900
FT                   /note="SIT HindIII"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6894 BP; 1630 A; 1780 C; 1830 G; 1654 T; 0 other;
     gaattcgagc tcggtacagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     aaaaagctcc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt
     cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga
     tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgcggaat
     tgtacccgcg gccgcaattc ccggggatcg aaagagcctg ctaaagcaaa aaagaagtca
     ccatgtcgtt tactttgacc aacaagaacg tgattttcgt tgccggtctg ggaggcattg
     gtctggacac cagcaaggag ctgctcaagc gcgatcccgt cgttttacaa cgtcgtgact
     gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
     gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg
     atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca
     cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg
     aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg
     ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat
     ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt
     atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc
     ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca
     gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg
     taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg
     gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg
     aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc
     acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc
     ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc
     gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata
     tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc
     cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg
     aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga
     tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct
     ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca
     aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca
     ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc
     cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt
     gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt
     ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga
     ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc
     cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag
     cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca
     tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg
     tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag
     gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc
     tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg
     cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg
     ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt
     ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac
     tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa
     gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt
     accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga
     ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct
     accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata
     caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa
     actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg
     accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg
     gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc
     agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc
     tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg
     acgactcctg gagcccgtca gtatcggcgg aattacagct gagcgccggt cgctaccatt
     accagttggt ctggtgtcaa aaataataat aaccgggcag gccatgtctg cccgtatttc
     gcgtaaggaa atccattatg tactatttaa aaaacacaaa cttttggatg ttcggtttat
     tctttttctt ttactttttt atcatgggag cctacttccc gtttttcccg atttggctac
     atgacatcaa ccatatcagc aaaagtgata cgggtattat ttttgccgct atttctctgt
     tctcgctatt attccaaccg ctgtttggtc tgctttctga caaactcggc ctcgactcta
     ggcggccgcg gggatccaga catgataaga tacattgatg agtttggaca aaccacaact
     agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     gttcaggggg aggtgtggga ggttttttcg gatcctctag agtcgacctg caggcatgca
     agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagt
//