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ID   PSVBGAL    preliminary; circular DNA; SYN; 6749 BP.
XX
AC   X65335; IG0161;
XX
DT   14-DEC-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSV-beta-galactosidase control - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-6749
RC   pSV-beta-galactosidase control
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-6749
RC   pSV-beta-galactosidase control
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
CC   NM (pSV-beta-galactosidase control)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE (color blue/white)
CC   PA (pSV)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. SV40, enhancer/promoter/small T antigen
FT                   3. E. coli, lacZ gene
FT                   -> pSV-beta-galactosidase control 6749bp"
FT   misc_binding    6743..6743
FT                   /note="SIT double EcoRI"
FT   promoter        0..0
FT                   /note="PRO SV40 enhancer and promoter"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_feature    637..3948
FT                   /note="E. coli lac operon sequence"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_feature    3737..3737
FT                   /note="ATG E. coli beta-galactosidase gene
FT                   transcription initiation site"
FT   CDS             3737..3939
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ),
FT                   partial"
FT   terminator      0..0
FT                   /note="TER E. coli beta-galactosidase gene
FT                   transcription termination site"
FT   misc_feature    3940..4085
FT                   /note="GEN SV40 small T antigen; partial"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   CDS             4712..5572
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 6749 BP; 1589 A; 1772 C; 1771 G; 1617 T; 0 other;
     gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
     gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttggg atctctataa
     tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
     acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
     actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
     cggtctggta ccggtgggtg aagaccagaa acagcacctc gaactgagcc gcgatattgc
     ccagcgtttc aacgcgctgt atggcgagat cgatcccgtc gttttacaac gtcgtgactg
     ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
     cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
     tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac
     ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
     aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg
     gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc tgagcgcatt
     tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg acggcagtta
     tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct cgttgctgca
     taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg atgatttcag
     ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact acctacgggt
     aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg
     tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga
     aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca
     caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg
     gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag gcgttaaccg
     tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg tgcaggatat
     cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc
     gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag ccaatattga
     aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc taccggcgat
     gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg
     gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc gctggatcaa
     atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca ccacggccac
     cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc
     gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc tgatcctttg
     cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact ggcaggcgtt
     tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc agtcgctgat
     taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg gcgatacgcc
     gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc
     gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg ggcaaaccat
     cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc actggatggt
     ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg
     taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc aactctggct
     cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc acatcagcgc
     ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc
     catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta ataagcgttg
     gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata aaaaacaact
     gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca ttggcgtaag
     tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta
     ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg cggtgctgat
     tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc ggaaaaccta
     ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg cgagcgatac
     accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag agcgggtaaa
     ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg cctgttttga
     ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga gcgaaaacgg
     tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg gcgacttcca
     gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc gccatctgct
     gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga ttggtggcga
     cgactcctgg agcccgtcag tatcggcgga attccagctg agcgccggtc gctaccatta
     ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc ccgtatttcg
     cgtaaggaaa tccattatgt actatttaaa aaacacaaac ttttggatgt tcggtttatt
     ctttttcttt tactttttta tcatgggagc ctacttcccg tttttcccga tttggctaca
     tgacatcaac catatcagca aaagtgatac gggtattatt tttgccgcta tttctctgtt
     ctcgctatta ttccaaccgc tgtttggtct gctttctgac aaactcggaa cttgtttatt
     gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     atcctctaga gtcgacctgc aggcatgcaa gctggcactg gccgtcgttt tacaacgtcg
     tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     aaacagctat gaccatgatt acgaattcg
//