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ID   PSVK3      preliminary; circular DNA; SYN; 3919 BP.
XX
AC   IG1120;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSVK3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSVKI from pSV2cat & pIC20H
RC   pSVKII from pSVKI & pBluescript KS+
RC   pSVKIII or PSVK3 from pSVKII & pIBI30, T7 promoter
RC   pSKcat from pIC20H & pCM3, Tn9 cat gene
RA   Mongkolsuk S.;
RT   "Novel eukaryotic expression vectors which permit single-stranded
RT   replication in Escherichia coli and in vitro translational
RT   analysis of cloned genes";
RL   Gene 70:313-319(1988).
XX
CC   NM (pSVK3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli)(mammal)
CC   CP ()
CC   FN (mapping gene)(expression shuttle)(transcription)
CC   SE ()
CC   PA (pBluescript II KS+)(pSV2cat)(pIBI130)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2cat remove HindIII-ScaI 3200bp,
FT                   \ cat gene/ScaI = 2106 4339/no HindIII/1800bp
FT                   2. pIC20H NruI-HindIII 2642bp 314..2722..234, MCS
FT                   -> pSVK1 4400bp
FT                   1. pSVK1 BamHI-PvuII 1486bp, MCS/SV40prom/polyA
FT                   \ pSV2cat 3370..4856
FT                   :fill in
FT                   2. pBluescript KS+ PvuII-PvuII 2519bp 978..2964..533,
FT                   \ M13ori/ori
FT                   -> pSVK2 3900bp
FT                   1. pSVK2 remove HindIII-XhoI 21bp 720..741, MCS/3900bp
FT                   fill in:
FT                   2. pIBI30 EcoRV-XhoI 83bp 235..318, T7 promoter/MCS
FT                   -> pSVK3 3919bp"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   promoter        352..369
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    352..>352
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   PstI-ApaI-XhoI-SacI, from pIBI130"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 small t antigen splice site"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    3917..3917
FT                   /note="SIT unique PvuII"
XX
SQ   Sequence 3919 BP; 1023 A; 917 C; 918 G; 1061 T; 0 other;
     ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatcgaa ttaatacgac
     tcattatagg gagatcgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca
     ggggccctcg agctcgactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaatg
     gcagaaattc gccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     caatgtatct tatcatgtct ggatcctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     ggccgattca ttaatgcag
//