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ID PSVL preliminary; circular DNA; SYN; 4896 BP.
XX
AC IG0102;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pSVL - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pJS77 from pBR322 & VSV
RC pJS100 from pMK2004 & pJS77
RC pJS114 from pN4 & pMK2004
RC pJS201 from pJS114 & pJS100
RC pJC32 from pSV53 & pMK2004
RC plasmid from pJC32
RC pJC119 from plasmid & pML2
RC pJC223 from pJS201 & pJC119
RA Sprague J., Condra J.H., Arnheiter H., Lazzarini R.A.;
RT "Expression of a recombinant DNA gene coding for the vesicular
RT stomatitis virus nucleocapsid protein";
RL J. Virol. 45:773-781(1983).
XX
RN [2]
RC plasmid from linker & pPyMT1
RC pMTSVL1 from pJC119 & plasmid
RC dl3, dl5, dl13 from pMTSVL1
RC [pSVL from pJC119]
RA Templeton D., Eckhart W.;
RT "N-terminal amino acid sequences of the polyoma middle-size T
RT antigen are important for protein kinase activity and cell
RT transformation";
RL Mol. Cell. Biol. 4:817-821(1984).
XX
RN [3]
RC pN3 from pBR322 & VSV N gene
RC pN4 from pBR322 & VSV N gene
RC pNS173 from pBR322 & VSV NS gene
RC pNS319 from pBR322 & VSV NS gene
RA Gallione C.J., Greene J.R., Iverson L.E., Rose J.K.;
RT "Nucleotide sequences of the mRNA's encoding the vesicular
RT stomatitis virus N and NS proteins";
RL J. Virol. 39:529-535(1981).
XX
RN [4]
RC pSV53 from pBR322 & SV40
RA Lai C.J.;
RT ;
RL Unpublished (1983).
XX
CC Uses first ATG in inserted DNA.
CC no poison sequences.
CC NM (pSVL)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli)(monkey COS cells)
CC CP ()
CC FN (expression shuttle)(expression transient)
CC SE ()
CC PA (pBR322)(SV40)(pJC119)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 BamHI 4361bp 376..376
FT 2. SV40 HindIII-BamHI 1040bp 1494..2534, late region
FT BamHI linker 6bp ggatcc:
FT -> pSV53 5401bp
FT 1. pBR322 4361bp
FT 2. E. coli, kan gene
FT -> pMK2004 6000bp
FT 1. pSV53 BamHI-BamHI 1040bp, SV40 late region
FT Klenow:Klenow
FT XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT XhoI-XhoI 1040bp, SV40 late region
FT 2. pMK2004 XhoI 6000bp, kan gene
FT -> pJC32 7000bp
FT 1. pJC32 remove XhoI-XhoI, pMK2004/7000bp
FT -> plasmid 7000bp
FT 1. plasmid BclI 7000bp, kan gene
FT 2. pML2 BamHI 2900bp 306..306
FT -> pJC119 9900bp
FT 1. pJC119 remove, 9900bp-
FT -> pSVL 4896bp"
FT rep_origin join(4837..4820,1..83)
FT /note="ORI SV40"
FT promoter 84..250
FT /note="PRO SV40 late genes"
FT misc_binding 270..270
FT /note="SIT unique PvuII"
FT misc_binding 294..294
FT /note="SIT unique KpnI"
FT intron >294..<1506
FT /note="SV40 VP1 intron"
FT misc_feature >294..<1506
FT /note="SPL SV40 splicing sequences"
FT misc_binding 768..768
FT /note="SIT unique EcoRV"
FT misc_binding 1506..1535
FT /note="MCS unique XhoI-XbaI-SmaI-SacI-BamHI"
FT polyA_signal >23..<877
FT /note="PLA SV40 late genes"
FT misc_binding 2047..2047
FT /note="SIT unique SalI"
FT rep_origin 2535..2535
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(3293..4153)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3614..3614
FT /note="SIT unique PstI"
FT misc_binding 3740..3740
FT /note="SIT unique PvuI"
FT misc_binding 4366..4366
FT /note="SIT unique EcoRI"
XX
SQ Sequence 4896 BP; 1208 A; 1176 C; 1180 G; 1332 T; 0 other;
gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaccggatc
gatccctcga gtctagaccc ggggagctcg gatccagaca tgataagata cattgatgag
tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
ctctacaaat gtggtatggc tgattatgat cgatcctcta cgccggacgc atcgtggccg
gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
aggtagatga cgaccatcag ggacagcttc aaaggccagc aaaaggccag gaaccgtaaa
aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg
acagcttatc atcgaagcag tagcaatcaa cccacacaag tggatctttc ctgtataatt
ttctattttc atgcttcatc ctcagtaagc acagcaagca tatgcagtta gcagacattt
tctttgcaca ctcaggccat tgtttgcagt acattgcatc aacaccagga tttaaggaag
aagcaaatac ctcagttgca tcccagaagc ctccaaagtc aggttgatga gcatatttta
ctccatcttc cattttcttg tacagagtat tcattttctt cattttttct tcatctcctc
ctttatcagg atgaaactcc ttgcattttt ttaaatatgc ctttctcatc agaggaatat
tcccccaggc actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt
ttaaaacttt atccatcttt gcaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
cctcactact tctggaatag ctcagaggcc gaggcg
//