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ID   PSVL       preliminary; circular DNA; SYN; 4896 BP.
XX
AC   IG0102;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pSVL - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJS77 from pBR322 & VSV
RC   pJS100 from pMK2004 & pJS77
RC   pJS114 from pN4 & pMK2004
RC   pJS201 from pJS114 & pJS100
RC   pJC32 from pSV53 & pMK2004
RC   plasmid from pJC32
RC   pJC119 from plasmid & pML2
RC   pJC223 from pJS201 & pJC119
RA   Sprague J., Condra J.H., Arnheiter H., Lazzarini R.A.;
RT   "Expression of a recombinant DNA gene coding for the vesicular
RT   stomatitis virus nucleocapsid protein";
RL   J. Virol. 45:773-781(1983).
XX
RN   [2]
RC   plasmid from linker & pPyMT1
RC   pMTSVL1 from pJC119 & plasmid
RC   dl3, dl5, dl13 from pMTSVL1
RC   [pSVL from pJC119]
RA   Templeton D., Eckhart W.;
RT   "N-terminal amino acid sequences of the polyoma middle-size T
RT   antigen are important for protein kinase activity and cell
RT   transformation";
RL   Mol. Cell. Biol. 4:817-821(1984).
XX
RN   [3]
RC   pN3 from pBR322 & VSV N gene
RC   pN4 from pBR322 & VSV N gene
RC   pNS173 from pBR322 & VSV NS gene
RC   pNS319 from pBR322 & VSV NS gene
RA   Gallione C.J., Greene J.R., Iverson L.E., Rose J.K.;
RT   "Nucleotide sequences of the mRNA's encoding the vesicular
RT   stomatitis virus N and NS proteins";
RL   J. Virol. 39:529-535(1981).
XX
RN   [4]
RC   pSV53 from pBR322 & SV40
RA   Lai C.J.;
RT   ;
RL   Unpublished (1983).
XX
CC   Uses first ATG in inserted DNA.
CC   no poison sequences.
CC   NM (pSVL)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)(monkey COS cells)
CC   CP ()
CC   FN (expression shuttle)(expression transient)
CC   SE ()
CC   PA (pBR322)(SV40)(pJC119)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 HindIII-BamHI 1040bp 1494..2534, late region
FT                   BamHI linker 6bp ggatcc:
FT                   -> pSV53 5401bp
FT                   1. pBR322 4361bp
FT                   2. E. coli, kan gene
FT                   -> pMK2004 6000bp
FT                   1. pSV53 BamHI-BamHI 1040bp, SV40 late region
FT                   Klenow:Klenow
FT                   XhoI linker 6bp ctcgag:XhoI linker 6bp ctcgag
FT                   XhoI-XhoI 1040bp, SV40 late region
FT                   2. pMK2004 XhoI 6000bp, kan gene
FT                   -> pJC32 7000bp
FT                   1. pJC32 remove XhoI-XhoI, pMK2004/7000bp
FT                   -> plasmid 7000bp
FT                   1. plasmid BclI 7000bp, kan gene
FT                   2. pML2 BamHI 2900bp 306..306
FT                   -> pJC119 9900bp
FT                   1. pJC119 remove, 9900bp-
FT                   -> pSVL 4896bp"
FT   rep_origin      join(4837..4820,1..83)
FT                   /note="ORI SV40"
FT   promoter        84..250
FT                   /note="PRO SV40 late genes"
FT   misc_binding    270..270
FT                   /note="SIT unique PvuII"
FT   misc_binding    294..294
FT                   /note="SIT unique KpnI"
FT   intron          >294..<1506
FT                   /note="SV40 VP1 intron"
FT   misc_feature    >294..<1506
FT                   /note="SPL SV40 splicing sequences"
FT   misc_binding    768..768
FT                   /note="SIT unique EcoRV"
FT   misc_binding    1506..1535
FT                   /note="MCS unique XhoI-XbaI-SmaI-SacI-BamHI"
FT   polyA_signal    >23..<877
FT                   /note="PLA SV40 late genes"
FT   misc_binding    2047..2047
FT                   /note="SIT unique SalI"
FT   rep_origin      2535..2535
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3293..4153)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3614..3614
FT                   /note="SIT unique PstI"
FT   misc_binding    3740..3740
FT                   /note="SIT unique PvuI"
FT   misc_binding    4366..4366
FT                   /note="SIT unique EcoRI"
XX
SQ   Sequence 4896 BP; 1208 A; 1176 C; 1180 G; 1332 T; 0 other;
     gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa
     ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa
     ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg
     gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca gaaggtacct
     aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc tgtcacgcca
     ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc aacgcctttt
     tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg ttgacgggaa
     acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa gtttagtctt
     tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga cctaattgct
     actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc tgctggagag
     gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg cctaacaacc
     tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat atctggggct
     cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt gagcgctgtt
     gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac tgttggttta
     tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta tgatatttta
     tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag acattggggt
     ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa tgacattcct
     aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga cagtttggca
     aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg gtataactct
     ttacaagatt actactctac tttgtctccc attaggccta caatggtgag acaagtagcc
     aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga tgaagcagac
     agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa tgtgcagtca
     ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac tgctcctcag
     tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct aaaccggatc
     gatccctcga gtctagaccc ggggagctcg gatccagaca tgataagata cattgatgag
     tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     ctctacaaat gtggtatggc tgattatgat cgatcctcta cgccggacgc atcgtggccg
     gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg
     aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag
     gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg
     cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg
     gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     aggtagatga cgaccatcag ggacagcttc aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg
     acagcttatc atcgaagcag tagcaatcaa cccacacaag tggatctttc ctgtataatt
     ttctattttc atgcttcatc ctcagtaagc acagcaagca tatgcagtta gcagacattt
     tctttgcaca ctcaggccat tgtttgcagt acattgcatc aacaccagga tttaaggaag
     aagcaaatac ctcagttgca tcccagaagc ctccaaagtc aggttgatga gcatatttta
     ctccatcttc cattttcttg tacagagtat tcattttctt cattttttct tcatctcctc
     ctttatcagg atgaaactcc ttgcattttt ttaaatatgc ctttctcatc agaggaatat
     tcccccaggc actcctttca agacctagaa ggtccattag ctgcaaagat tcctctctgt
     ttaaaacttt atccatcttt gcaaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     cctcactact tctggaatag ctcagaggcc gaggcg
//