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ID PSVSPORT1 preliminary; circular DNA; SYN; 3160 BP.
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AC U14626; IG1152;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSV-SPORT1 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSV-SPORT1 from pKO-neo & pSPORT1
RA Crouse J.E.;
RT "Plasmid pSV-SPORT1";
RL GibcoBRL Catalogue 93:7p16-7p17(1993).
RL Crouse J.E., Life Technologies Inc., Gaithersburg MD 20877.
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RN [2]
RC pSV-SPORT1 from pKO-neo & pSPORT1
RA D'Alessio J.M.;
RT ;
RL Unpublished (1994).
RL Life Technologies, Inc. Catalogue.
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RN [3]
RC pSV-SPORT1, lacZ gene
RA Horton M.E.;
RT ;
RL Submitted (12-SEP-1994) by:
RL Horton M.E., Life Technologies, Inc., Technical Services,
RL 8400 Helgerman Court, Gaithersburg, MD 20884-9980, USA.
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CC NCBI gi: 540252
CC NM (pSV-SPORT1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (BRL)
CC HO (E.coli DH5alpha)
CC CP ()
CC FN (expression transient)(shuttle bacteria/mammal)
CC FN (in vitro mutagenesis)(sequencing dideoxy)
CC FN (cloning random/oriented cDNA)
CC SE ()
CC PA (pKO-neo)
CC BR (pSPORT1)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 remove small EcoRI-HindIII 31bp
FT \ 4360..4361..30, 4330bp
FT 2. SV40 HindIII-PvuII 344bp 5172..5243..273,
FT \ ori/early promoter
FT 3. E. coli AluI-EcoRI 95bp, lacUV5 promoter
FT \ ctcactcattaggcaccccaggctttacactttatgcttccggctcgtat
FT \ aatgtgtggaattgtgagcggataacaatttcacacaggaaacag
FT -> plasmid 4769bp
FT 1. plasmid BamHI-HindIII 4423bp, SV40 ori/early prom
FT \ pBR322 376..4360..-..- [remove 346bp]
FT 2. SV40 MboI-MboI 610bp 4101..4711, intron [615bp]
FT Klenow:Klenow
FT HindIII linker 10bp nnaagcttnn:HindIII linker 10bp
FT \ nnaagcttnn
FT HindIII-HindIII 615bp
FT HinfI-HindIII 143bp 4570..4713, [147bp]
FT \ SV40 small t intron
FT 3. SV40 BamHI-HinfI 291bp 2534..2825, polyA [289bp]
FT -> pko 4857bp
FT 1. Tn5 HindIII-BamHI 1861bp 1196..3057,
FT \ neo/kan gene with BglII
FT -> plasmid2
FT 1. pko
FT 2. plasmid2 HindIII-BamHI 1861bp 1196..3057,
FT \ neo/kan gene with BglII
FT -> pKO-neo 3747bp
FT 1. pSPORT1 AatII-PstI 89bp 196..285, MCS
FT 2. pKO-neo remove PstI-AatII 677bp, pBR322 3614..4291
FT \ 3070bp
FT -> pSV1-SPORT 3160bp"
FT promoter 23..166
FT /note="PRO SV40 early genes enhancer"
FT promoter 170..232
FT /note="PRO SV40 early genes"
FT misc_binding 231..231
FT /note="SIT unique DsaI"
FT misc_binding 231..231
FT /note="SIT unique NcoI"
FT rep_origin 260..286
FT /note="ORI SV40"
FT misc_binding 270..270
FT /note="SIT unique SfiI"
FT misc_binding 321..321
FT /note="SIT unique StuI"
FT misc_binding 324..324
FT /note="SIT unique AvrII"
FT promoter 345..364
FT /note="PRO bacteriophage Sp6"
FT misc_binding 371..466
FT /note="MCS PstI-KpnI-RsrII-BspMII-EcoRI-SmaI-SalI-
FT SstI-SpeI-NotI-XmaIII-XbaI-HindIII-SnaBI-SplI-MluI"
FT promoter complement(476..496)
FT /note="PRO bacteriophage T7"
FT CDS complement(>2500..3536)
FT /note="GEN SV40 small t-intron region"
FT misc_feature complement(>500..<880)
FT /note="SPL SV40 72 base insertion"
FT misc_binding 629..629
FT /note="SIT unique NdeI"
FT misc_binding 776..776
FT /note="SIT unique BclI"
FT misc_binding 777..777
FT /note="SIT unique MamI"
FT misc_binding 869..869
FT /note="SIT unique MunI"
FT misc_binding 880..880
FT /note="SIT unique HpaI"
FT polyA_signal 912..919
FT /note="PLA SV40 early genes"
FT polyA_signal 941..948
FT /note="PLA SV40 early genes"
FT rep_origin 1146..1170
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(2003..2863)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 2296..2296
FT /note="SIT unique FspI"
FT misc_binding 2443..2443
FT /note="SIT unique PvuI"
FT misc_binding 2554..2554
FT /note="SIT unique ScaI"
FT misc_binding 2671..2671
FT /note="SIT unique XmnI"
FT promoter complement(>3050..<3154)
FT /note="PRO E. coli lacUV5 gene"
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SQ Sequence 3160 BP; 820 A; 775 C; 724 G; 841 T; 0 other;
ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggcgta
ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctatttag gtgacactat
agaaggtacg cctgcaggta ccggtccgga attcccgggt cgacgagctc actagtcggc
ggccgctcta gaggatccaa gcttacgtac gcgtgcatgc gacgtcatag ctctctccct
atagtgagtc gtattataag ctagcttggg atctttgtga aggaacctta cttctgtggt
gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
ttaagtgtat aatgtgttaa actagctgca tatgcttgct gcttgagagt tttgcttact
gagtatgatt tatgaaaata ttatacacag gagctagtga ttctaattgt ttgtgtattt
tagattcaca gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc
ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcctg
cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
ggctacacta gaaggacagt atttggtatc tgcggccagt taccttcgga aaaagagttg
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatacga ggccctttca
ctcattaggc accccaggct ttacacttta tagcttccgg ctcgtataat gtgtggaatt
gtgagcggat aacaatttca cacaggaaac agcatcgatg
//