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ID   PSVXBP1    preliminary; circular DNA; SYN; 6343 BP.
XX
AC   IG9851;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pSVXB-p1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSVXB-p1 from SV40 ori/Tantigen & pUC18 & pBR327
RC   pSVXBenh from pSVXB-p1 & pbetaGXSV512, SV40 enhancer
RC   pSVXB1504, pSVXB912, pSVXB490, pSVXB276 from pSVXB-p1 & HBV
RC   pUC18 HBV912 from pUC18 & HBV
RC   pHB23, pHB31 from pSVXB-p1 & pUC18 HBV912
RC   [pUCTag from pUC18 & pSVXB-p1]
RA   Tognoni A., Cattaneo R., Serfling E., Schaffner W.;
RT   "A novel expression selection approach allows precise mapping of
RT   the hepatitis B virus enhancer";
RL   Nucleic Acids Res. 13:7457-7472(1985).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSVXB-p1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (SV40)(pBR327)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. SV40 BamHI-EcoRI 4492bp 2534..5243..1783,
FT                   \ ori/T ant
FT                   fill in:
FT                   2. pBR327 AvaI-EcoRI 1847bp 1425..3272, ori/amp gene
FT                   fill in:
FT                   -> pSVXB-p1 6339bp [AvaI, no BamHI]"
FT   -               1..4492
FT                   /note="SV40 2534..5243..1782 4492bp
FT                   EcoRI = G^AATTC"
FT   -               4493..6343
FT                   /note="pBR327 1425..3273..2 1851bp complement
FT                   AvaI = C^YCGR G
FT                   BamHI =     G^GATCC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 6343 BP; 1787 A; 1390 C; 1321 G; 1845 T; 0 other;
     gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     catgaacaga ctgtgaggac tgaggggcct gaaatgagcc ttgggactgt gaatcaatgc
     ctgtttcatg ccctgagtct tccatgttct tctccccacc atcttcattt ttatcagcat
     tttcctggct gtcttcatca tcatcatcac tgtttcttag ccaatctaaa actccaattc
     ccatagccac attaaacttc attttttgat acactgacaa actaaactct ttgtccaatc
     tctctttcca ctccacaatt ctgctctgaa tactttgagc aaactcagcc acaggtctgt
     accaaattaa cataagaagc aaagcaatgc cactttgaat tattctcttt tctaacaaaa
     actcactgcg ttccaggcaa tgctttaaat aatctttggg cctaaaatct atttgtttta
     caaatctggc ctgcagtgtt ttaggcacac tgtactcatt catggtgact attccagggg
     gaaatatttg agttctttta tttaggtgtt tcttttctaa gtttacctta acactgccat
     ccaaataatc ccttaaattg tccaggttat taattccctg acctgaaggc aaatctctgg
     actcccctcc agtgcccttt acatcctcaa aaactactaa aaactggtca atagctactc
     ctagctcaaa gttcagcctg tccaagggca aattaacatt taaagctttc cccccacata
     attcaagcaa agcagctgct aatgtagttt taccactatc aattggtcct ttaaacagcc
     agtatctttt tttaggaatg ttgtacacca tgcattttaa aaagtcatac accactgaat
     ccattttggg caacaaacag tgtagccaag caactccagc catccattct tctatgtcag
     cagagcctgt agaaccaaac attatatcca tcctatccaa aagatcatta aatctgtttg
     ttaacatttg ttctctagtt aattgtaggc tatcaacccg ctttttagct aaaacagtat
     caacagcctg ttggcatatg gttttttggt ttttgctgtc agcaaatata gcagcatttg
     cataatgctt ttcatggtac ttatagtggc tgggctgttc ttttttaata cattttaaac
     acatttcaaa actgtactga aattccaagt acatcccaag caataacaac acatcatcac
     attttgtttc cattgcatac tctgttacaa gcttccagga cacttgttta gtttcctctg
     cttcttctgg attaaaatca tgctccttta acccacctgg caaactttcc tcaataacag
     aaaatggatc tctagtcaag gcactataca tcaaatattc cttattaacc cctttacaaa
     ttaaaaagct aaaggtacac aatttttgag catagttatt aatagcagac actctatgcc
     tgtgtggagt aagaaaaaac agtatgttat gattataact gttatgccta cttataaagg
     ttacagaata tttttccata attttcttgt atagcagtgc agctttttcc tttgtggtgt
     aaatagcaaa gcaagcaaga gttctattac taaacacagc atgactcaaa aaacttagca
     attctgaagg aaagtccttg gggtcttcta cctttctctt cttttttgga ggagtagaat
     gttgagagtc agcagtagcc tcatcatcac tagatggcat ttcttctgag caaaacaggt
     tttcctcatt aaaggcattc caccactgct cccattcatc agttccatag gttggaatct
     aaaatacaca aacaattaga atcagtagtt taacacatta tacacttaaa aattttatat
     ttaccttaga gctttaaatc tctgtaggta gtttgtccaa ttatgtcaca ccacagaagt
     aaggttcctt cacaaagatc aagtccaaac cacattctaa agcaatcgaa gcagtagcaa
     tcaacccaca caagtggatc tttcctgtat aattttctat tttcatgctt catcctcagt
     aagcacagca agcatatgca gttagcagac attttctttg cacactcagg ccattgtttg
     cagtacattg catcaacacc aggatttaag gaagaagcaa atacctcagt tgcatcccag
     aagcctccaa agtcaggttg atgagcatat tttactccat cttccatttt cttgtacaga
     gtattcattt tcttcatttt ttcttcatct cctcctttat caggatgaaa ctccttgcat
     ttttttaaat atgcctttct catcagagga atattccccc aggcactcct ttcaagacct
     agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcaaag
     ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     gcctggggac tttccacacc ctaactgaca cacattccac agctggttct ttccgcctca
     gaaggtacct aaccaagttc ctctttcaga ggttatttca ggccatggtg ctgcgccggc
     tgtcacgcca ggcctccgtt aaggttcgta ggtcatggac tgaaagtaaa aaaacagctc
     aacgcctttt tgtgtttgtt ttagagcttt tgctgcaatt ttgtgaaggg gaagatactg
     ttgacgggaa acgcaaaaaa ccagaaaggt taactgaaaa accagaaagt taactggtaa
     gtttagtctt tttgtctttt atttcaggtc catgggtgct gctttaacac tgttggggga
     cctaattgct actgtgtctg aagctgctgc tgctactgga ttttcagtag ctgaaattgc
     tgctggagag gccgctgctg caattgaagt gcaacttgca tctgttgcta ctgttgaagg
     cctaacaacc tctgaggcaa ttgctgctat aggcctcact ccacaggcct atgctgtgat
     atctggggct cctgctgcta tagctggatt tgcagcttta ctgcaaactg tgactggtgt
     gagcgctgtt gctcaagtgg ggtatagatt ttttagtgac tgggatcaca aagtttctac
     tgttggttta tatcaacaac caggaatggc tgtagatttg tataggccag atgattacta
     tgatatttta tttcctggag tacaaacctt tgttcacagt gttcagtatc ttgaccccag
     acattggggt ccaacacttt ttaatgccat ttctcaagct ttttggcgtg taatacaaaa
     tgacattcct aggctcacct cacaggagct tgaaagaaga acccaaagat atttaaggga
     cagtttggca aggtttttag aggaaactac ttggacagta attaatgctc ctgttaattg
     gtataactct ttacaagatt actactctac tttgtctccc attaggccta caatggtgag
     acaagtagcc aacagggaag ggttgcaaat atcatttggg cacacctatg ataatattga
     tgaagcagac agtattcagc aagtaactga gaggtgggaa gctcaaagcc aaagtcctaa
     tgtgcagtca ggtgaattta ttgaaaaatt tgaggctcct ggtggtgcaa atcaaagaac
     tgctcctcag tggatgttgc ctttacttct aggcctgtac ggaagtgtta cttctgctct
     aaaagcttat gaagatggcc ccaacaaaaa gaaaaggaag ttgtccaggg gcagctccca
     aaaaaccaaa ggaaccagtg caagtgccaa agctcgtcat aaaaggagga atagaagttc
     taggagttaa aactggagta gacagcttca ctgaggtgga gtgcttttta aatcctcaaa
     tgggcaatcc tgatgaacat caaaaaggct taagtaaaag cttagcagct gaaaaacagt
     ttacagatga ctctccagac aaagaacaac tgccttgcta cagtgtggct agaattcttg
     aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     aggggggcgg agcctatgga aaaacgccag caacgcggcc cga
//