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ID   PSZ62      preliminary; circular DNA; SYN; 6129 BP.
XX
AC   ATCC77120;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli plasmid vector pSZ62 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSZ32 from pBR322 & yeast chromosome & pYeleu10, LEU2 gene
RC   pSZ57, pSZ58 from pBR322 & pYeleu10, LEU2 gene
RC   pSZ62, pSZ63 from pBR322 & yeast HIS3 gene
RC   pSZ64 from pSZ63
RC   pSZ500 from pSZ62
RC   pWJ12 from pSZ62 & yeast SUP3 gene
RA   Orr-Weaver T.L., Szostak J.W., Rothstein R.J.;
RT   "Yeast transformation: a model system for the study of
RT   recombination";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:6354-6358(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Constructed by inserting a 1.7 kb HIS3-containing BamHI fragment into
CC   the BamHI site of pBR322.
CC   Integration YI-type shuttle vector. (ATCC staff)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--6.1; BamHI--4.4, 1.7; BglII--5.4, 0.7; SalI/XhoI--4.6, 1.5;
CC   EcoRI/SalI--3.7, 2.4; SalI--6.1. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSZ62)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC   HO (Saccharomyces cerevisiae LL20)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pYAC4)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. pYAC4 BamHI-BamHI 1768bp 4238..6006, HIS3 gene
FT                   -> pSZ62 6129bp"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC"
FT   -               376..2143
FT                   /note="pYAC4 4238..6005 1768bp
FT                   BamHI = G^GATCC"
FT   -               2144..6129
FT                   /note="pBR322 376..4361 3986bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
XX
SQ   Sequence 6129 BP; 1480 A; 1615 C; 1499 G; 1535 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tcggggacac caaatatggc gatctcggcc ttttcgtttc
     ttggagctgg gacatgtttg ccatcgatcc atctaccacc agaacggccg ttagatctgc
     tgccaccgtt gtttccaccg aagaaaccac cgttgccgta accaccacga cggttgttgc
     taaagaagct gccaccgcca cggccaccgt tgtagccgcc gttgttgtta ttgtagttgc
     tcatgttatt tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct
     cgttgttgtc gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa
     atgcttttct tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt
     tcccgttcct ccatctcttt tatatttttt ttctcgatcg agttcaagag aaaaaaaaag
     aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg
     cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg
     tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact
     acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc
     ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc
     gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg
     ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg
     cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa
     atcctgatcc aaaccttttt actccacgcg ccagtagggc ctctttaaaa gcttgaccga
     gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact
     caacgattag cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc
     ctatacctgt gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg
     cctctttttc tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg
     caatctgaat cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct
     ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac
     tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca
     tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg
     tactagagga ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac
     ttccctgact aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc
     tcttaaaacg agaattaaga aaaagtcgtc atctttcgat aagtttttcc cacagcaaag
     caatagtaga aaaaaacaat gggaaacgtt gaatgaagac aaagcgtcgt ggtttaaaag
     gaaatacgct cacgtacatg ctagggaaca ggaccgtgca gcggatcctc tacgccggac
     gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
     cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
     cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
     gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
     cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
     gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
     gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
     atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
     caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
     atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
     ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
     aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
     tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
     ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     cttcaagaa
//