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ID PSZ62 preliminary; circular DNA; SYN; 6129 BP.
XX
AC ATCC77120;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Saccharomyces/E.coli plasmid vector pSZ62 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSZ32 from pBR322 & yeast chromosome & pYeleu10, LEU2 gene
RC pSZ57, pSZ58 from pBR322 & pYeleu10, LEU2 gene
RC pSZ62, pSZ63 from pBR322 & yeast HIS3 gene
RC pSZ64 from pSZ63
RC pSZ500 from pSZ62
RC pWJ12 from pSZ62 & yeast SUP3 gene
RA Orr-Weaver T.L., Szostak J.W., Rothstein R.J.;
RT "Yeast transformation: a model system for the study of
RT recombination";
RL Proc. Natl. Acad. Sci. U.S.A. 78:6354-6358(1981).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Constructed by inserting a 1.7 kb HIS3-containing BamHI fragment into
CC the BamHI site of pBR322.
CC Integration YI-type shuttle vector. (ATCC staff)
CC Restriction digests of the clone give the following sizes (kb):
CC EcoRI--6.1; BamHI--4.4, 1.7; BglII--5.4, 0.7; SalI/XhoI--4.6, 1.5;
CC EcoRI/SalI--3.7, 2.4; SalI--6.1. (ATCC staff)
CC Medium is 1227 LB plus ampicillin.
CC NM (pSZ62)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli HB101)(Saccharomyces cerevisiae)(E.coli)
CC HO (Saccharomyces cerevisiae LL20)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(pYAC4)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 BamHI 4361bp 376..376
FT 2. pYAC4 BamHI-BamHI 1768bp 4238..6006, HIS3 gene
FT -> pSZ62 6129bp"
FT - 1..375
FT /note="pBR322 1..375 375bp
FT BamHI = G^GATCC"
FT - 376..2143
FT /note="pYAC4 4238..6005 1768bp
FT BamHI = G^GATCC"
FT - 2144..6129
FT /note="pBR322 376..4361 3986bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI-SalI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT CDS 0..0
FT /note="ANT yeast HIS3 gene"
XX
SQ Sequence 6129 BP; 1480 A; 1615 C; 1499 G; 1535 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tcggggacac caaatatggc gatctcggcc ttttcgtttc
ttggagctgg gacatgtttg ccatcgatcc atctaccacc agaacggccg ttagatctgc
tgccaccgtt gtttccaccg aagaaaccac cgttgccgta accaccacga cggttgttgc
taaagaagct gccaccgcca cggccaccgt tgtagccgcc gttgttgtta ttgtagttgc
tcatgttatt tctggcactt cttggttttc ctcttaagtg aggaggaaca taaccattct
cgttgttgtc gttgatgctt aaattttgca cttgttcgct cagttcagcc ataatatgaa
atgcttttct tgttgttctt acggaatacc acttgccacc tatcaccaca actaactttt
tcccgttcct ccatctcttt tatatttttt ttctcgatcg agttcaagag aaaaaaaaag
aaaaagcaaa aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg
cattaccttg tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg
tatacatata tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact
acataagaac acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc
ttatggcaac cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc
gcagacaatc aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg
ggatcatctc gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg
cgtacggcct gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa
atcctgatcc aaaccttttt actccacgcg ccagtagggc ctctttaaaa gcttgaccga
gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact
caacgattag cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc
ctatacctgt gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg
cctctttttc tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg
caatctgaat cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct
ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac
tttatataat gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca
tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg
tactagagga ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac
ttccctgact aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc
tcttaaaacg agaattaaga aaaagtcgtc atctttcgat aagtttttcc cacagcaaag
caatagtaga aaaaaacaat gggaaacgtt gaatgaagac aaagcgtcgt ggtttaaaag
gaaatacgct cacgtacatg ctagggaaca ggaccgtgca gcggatcctc tacgccggac
gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca
cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt
gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg
cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct
gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc
gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac
atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg
caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac
atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat
ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt
aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa
tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg
ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
cttcaagaa
//