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ID PT3T7BM preliminary; circular DNA; SYN; 2746 BP.
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AC IG2010;
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DT 03-FEB-1994 (Rel. 8, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pT3T7BM - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pT3T7BM from pT3T7-lac
RA Hoeltke X.;
RT ;
RL Submitted (20-NOV-1986) by:
RL Hoeltke ., Stratagene.
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CC NM (pT3T7BM)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Boehringer)
CC HO (E.coli JM83)(E.coli JM105)(E.coli JM109)
CC CP (high)
CC FN (transcription both strands)
CC SE (color blue/white)
CC PA (pT3T7-lac)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pT3T7-lac remove EcoRI-HindIII 51bp 6..57,
FT \ MCS/2695bp
FT 2. oligo HindIII-EcoRI 51bp, MCS
FT -> pT3T7BM 2746bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation site"
FT misc_feature 1..66
FT /note="MCS Boehringer polylinker"
FT promoter complement(66..85)
FT /note="PRO bacteriophage T3 promoter"
FT misc_feature 69..69
FT /note="bacteriophage T3 transcription initiation site"
FT promoter complement(122..157)
FT /note="PRO E. coli lac gene"
FT misc_feature complement(148..148)
FT /note="E. coli lacZ transcription initiation site"
FT mutation 257..257
FT /note="A in pT3T7lac; G in pUCl9 base 257"
FT mutation 317..317
FT /note="G in pT3T7lac; A in pUCl9 base 317"
FT rep_origin complement(440..440)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 448..448
FT /note="SIT unique AflIII"
FT mutation 627..627
FT /note="G in pT3T7lac; C in pUCl9 base 567"
FT mutation 799..799
FT /note="C in pT3T7lac; G in pUCl9 base 739"
FT misc_feature complement(1271..2128)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1421..1421
FT /note="SIT unique Cfr101"
FT misc_binding 1563..1563
FT /note="SIT unique AvlII"
FT misc_binding 1621..1621
FT /note="SIT unique ScaI"
FT misc_binding 1940..1940
FT /note="SIT unique Asp700I or XmnI"
FT misc_binding 2145..2145
FT /note="SIT unique SspI"
FT misc_binding 2263..2263
FT /note="SIT unique AatII"
FT misc_binding 2317..2317
FT /note="SIT unique DraII"
FT misc_binding 2320..2320
FT /note="SIT unique PvuI"
FT misc_binding 2512..2512
FT /note="SIT unique NdeI"
FT misc_binding 2564..2564
FT /note="SIT unique NarI"
FT misc_binding 2567..2567
FT /note="SIT unique BbeI"
FT CDS complement(2476..2746)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT CDS complement(1..111)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT promoter join(2729..2746,1..4)
FT /note="PRO bacteriophage T7"
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SQ Sequence 2746 BP; 680 A; 685 C; 701 G; 680 T; 0 other;
gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc
ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc
ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//