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ID   PT3T7LAC   preliminary; circular DNA; SYN; 2746 BP.
XX
AC   IG0175;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT3T7-lac - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pJEB1, pJEB4, pJEB6 from pBR322 & Sp6
RA   Brown J.E., Klement J.F., McAllister W.T.;
RT   "Sequences of three promoters for the bacteriophage SP6 RNA
RT   polymerase";
RL   Nucleic Acids Res. 14:3521-3526(1986).
XX
RN   [2]
RC   from phage Sp6
RC   from phage T7
RC   from phage T3
RA   Chamberlin M.J., Ryan T.;
RT   "Bacteriophage DNA-dependent RNA polymerases";
RL   The Enzymes 15:87-108(1982).
XX
RN   [3]
RC   phage T7 from E. coli
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the
RT   locations of T7 genetic elements";
RL   J. Mol. Biol. 166:477-535(1983).
XX
RN   [4]
RC   from T7 promoter
RA   Martin C.T., Coleman J.E.;
RT   "Kinetic analysis of T7 RNA polymerase-promoter interactions with
RT   small synthetic promoters";
RL   Biochemistry 26:2690-2696(1987).
XX
RN   [5]
RC   pSP64-WSN-NS, pSPR1-801-d6 from pSP64, SP6 promoter
RA   Parvin J.D., Smith F.I., Palese P.;
RT   "Rapid RNA sequencing using double-stranded template DNA, SP6
RT   polymerase, and 3'-deoxynucleotide triphosphates";
RL   DNA 5:167-171(1986).
XX
RN   [6]
RC   pT3T7-lac from pUC19
RA   Hoeltke X.;
RT   ;
RL   Submitted (20-NOV-1986) by:
RL   Hoeltke ., Stratagene.
XX
CC   The vector BlueScribe is from STRATAGENE.
CC   old vector pT3T7lac from BOEHRINGER.
CC   NM (pT3T7-lac)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Stratagene)
CC   HO (E.coli JM83)(E.coli JM105)(E.coli JM109)
CC   CP (high)
CC   FN (transcription both strands)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp, MCS
FT                   2. T3, promoter
FT                   3. T7, promoter
FT                   -> pT3T7-lac 2746bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation site"
FT   misc_feature    1..66
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   BspMI-PstI-SphI-HindIII from pUC19/M13mp19"
FT   promoter        complement(66..85)
FT                   /note="PRO bacteriophage T3 promoter"
FT   misc_feature    69..69
FT                   /note="bacteriophage T3 transcription initiation site"
FT   promoter        complement(122..157)
FT                   /note="PRO E. coli lac gene"
FT   misc_feature    complement(148..148)
FT                   /note="E. coli lacZ transcription initiation site"
FT   mutation        257..257
FT                   /note="A in pT3T7lac; G in pUCl9 base 257"
FT   mutation        317..317
FT                   /note="G in pT3T7lac; A in pUCl9 base 317"
FT   rep_origin      complement(440..440)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    448..448
FT                   /note="SIT unique AflIII"
FT   mutation        627..627
FT                   /note="G in pT3T7lac; C in pUCl9 base 567"
FT   mutation        799..799
FT                   /note="C in pT3T7lac; G in pUCl9 base 739"
FT   misc_feature    complement(1271..2128)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1421..1421
FT                   /note="SIT unique Cfr101"
FT   misc_binding    1563..1563
FT                   /note="SIT unique AvlII"
FT   misc_binding    1621..1621
FT                   /note="SIT unique ScaI"
FT   misc_binding    1940..1940
FT                   /note="SIT unique Asp700I or XmnI"
FT   misc_binding    2145..2145
FT                   /note="SIT unique SspI"
FT   misc_binding    2263..2263
FT                   /note="SIT unique AatII"
FT   misc_binding    2317..2317
FT                   /note="SIT unique DraII"
FT   misc_binding    2320..2320
FT                   /note="SIT unique PvuI"
FT   misc_binding    2512..2512
FT                   /note="SIT unique NdeI"
FT   misc_binding    2564..2564
FT                   /note="SIT unique NarI"
FT   misc_binding    2567..2567
FT                   /note="SIT unique BbeI"
FT   CDS             complement(2476..2746)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             complement(1..111)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   promoter        join(2729..2746,1..4)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 2746 BP; 680 A; 685 C; 701 G; 680 T; 0 other;
     gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc
     ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc
     ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata
//