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ID   PT3T7LUC   preliminary; circular DNA; SYN; 4674 BP.
XX
AC   IG1121;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT3T7-luc - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [lambda gLuc1 from lambda & luc gene]
RC   [lambda Luc23 from lambda & luc gene]
RC   pLuc23-4 from pUC19 & lambda Luc23
RC   pLuc23A from pUC13 & lambda Luc23
RC   pLuc23B from pUC13 & lambda Luc23
RC   pJD180, pJD181, pJD182 from pUC19 & lambda gLuc1
RC   pJD183 from pUC13 & lambda gLuc1
RC   pJD200 from pUC18 & pJD180 & linker
RC   pJD201 from pJD200 & pLuc23-4
RC   pJD202 from pUC18 & pJD201
RC   pJD204, pJD206 from pJD202 & linker
RC   pJD205, pJD207 from pJD201 & linker
RC   pSV2A from pSV2
RC   pSV232A from pSV2
RC   pSV2A/L-Adelta5' from pSV2A & pJD206
RC   pSV232AL-Adelta5' from pSV232A & pJD206
RC   pRSV from RSV
RC   pRSVL-Adelta5' from pRSV & pJD206
RC   [pRSV-L from pRSV & luciferase gene]
RA   De Wet J.R., Wood K.V., DeLuca M., Helinski D.R., Subramani S.;
RT   "Firefly luciferase gene: structure and expression in mammalian
RT   cells";
RL   Mol. Cell. Biol. 7:725-737(1987).
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CC   NM (pT3T7-luc)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pT3T7-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. T3 promoter
FT                   3. T7 promoter
FT                   4. luc gene
FT                   -> pT3T7-luc 4674bp"
FT   promoter        66..85
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    69..69
FT                   /note="bacteriophage T3 transcription initiation site"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT                   LUC gene-BamHI-SmaI-KpnI-ApaI-XhoI-SalI-ClaI-
FT                   HindIII-EcoRV-EcoRI"
FT   misc_feature    complement(1..1)
FT                   /note="bacteriophage T7 transcription initiation site"
FT   promoter        complement(4..2729)
FT                   /note="PRO bacteriophage T7"
FT   CDS             111..2476
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT NarI"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_binding    0..0
FT                   /note="SIT AatII"
FT   CDS             1271..2128
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT XmnI"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   misc_binding    0..0
FT                   /note="SIT BglI"
FT   misc_binding    0..0
FT                   /note="SIT HgiEII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT AflIII"
FT   promoter        0..0
FT                   /note="PRO E. coli lacP gene"
XX
SQ   Sequence 4674 BP; 1239 A; 1092 C; 1141 G; 1202 T; 0 other;
     aagcttgcat gcctgcaggt cgactctaga ggatccgagc ttggaattcc tttgtgttac
     attcttgaat gtcgctcgca gtgacattag cattccggta ctgttggtaa aatggaagac
     gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
     gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
     gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
     accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
     ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
     gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
     gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
     agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
     tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     aagggcggaa agtccaaatt gtaaaatgta actgtattca gcgatgacga aattcttagc
     tattgtaata ttatatgcaa attgatgaat ggtaattttg taattgtggg tcactgtact
     attttaacga ataataaaat caggtatagg taactaaaaa ggaattcgag ctcggtaccc
     ggggatcccc gggtaccggg ccccccctcg aggtcgacgg tatcgataag cttgatatcg
     aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga acaa
//