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ID   PT70       preliminary; circular DNA; SYN; 4085 BP.
XX
AC   IG1122;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pT7-0 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGP1-1 from pKC30 & T7 phage gene I & linker
RC   pGP1-2 from pGP1-1 & pKB280-cI857 & pACYC177
RC   from pGP1-2 & pT7-1
RC   pGP5-1 from pT7-1 & T7 genes 5/5.3
RC   from pGP1-2 & pGP5-1
RC   [pT7-0, pT7-1, pT7-2 from T7]
RA   Tabor S., Richardson C.C.;
RT   "A bacteriophage T7 RNA polymerase/promoter system for controlled
RT   exclusive expression of specific genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:1074-1078(1985).
XX
CC   Cut with BanII, SalI, PvuII separately to
CC   make 3 RNA transcripts using T7 RNA polymerase.
CC   NM (pT7-0)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)
CC   SE ()
CC   PA (pBR322)
CC   BR (pT7-1)(pT7-2)(pT7-7)(pT7-SC)(pT7-SCII)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. T7, promoter
FT                   -> pT7-0 4085bp"
FT   misc_binding    0..0
FT                   /note="SIT AatII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT NruI"
FT   misc_binding    0..0
FT                   /note="SIT AvaI"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7 (phi-10),
FT                   between EcoRI and BamHI sites of pBR322"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3331..3331
FT                   /note="SIT PstI"
FT   misc_binding    3457..3457
FT                   /note="SIT PvuI"
FT   misc_binding    3568..3568
FT                   /note="SIT ScaI"
XX
SQ   Sequence 4085 BP; 937 A; 1124 C; 1064 G; 960 T; 0 other;
     ttcttcccga tccgtccgat tcgaacttct gatagacttc gaaattaata cgactcacta
     tagggagacc acaacggttt ccctctagac ggatcgggga tcctctacgc cggacgcatc
     gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     gtggcaggcc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg
     cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct
     tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
     cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
     ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
     ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
     gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
     catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
     tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
     taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
     gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
     gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
     gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
     cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
     ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
     tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
     attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
     cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
     tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaattcccc
     cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     aagaa
//