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ID   PT71       preliminary; circular DNA; SYN; 2780 BP.
XX
AC   IG1123;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGP1-1 from pKC30 & T7 phage gene I & linker
RC   pGP1-2 from pGP1-1 & pKB280-cI857 & pACYC177
RC   from pGP1-2 & pT7-1
RC   pGP5-1 from pT7-1 & T7 genes 5/5.3
RC   from pGP1-2 & pGP5-1
RC   [pT7-0, pT7-1, pT7-2 from T7]
RA   Tabor S., Richardson C.C.;
RT   "A bacteriophage T7 RNA polymerase/promoter system for controlled
RT   exclusive expression of specific genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:1074-1078(1985).
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CC   Transcription begins at 2324 and goes rightward.
CC   AMP 201..1061.
CC   MCS 2333..2383 follows T7 promoter.
CC   2147..2284 and 2643..2780 repeat.
CC   NM (pT7-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)
CC   SE ()
CC   PA (pBR322)
CC   BR (pT7-0)(pT7-2)(pT7-7)(pT7-SC)(pT7-SCII)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. T7, promoter
FT                   -> pT7-1 2780bp"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    34..79
FT                   /note="MCS EcoRI-BanII-SacI-AvaI-SmaI-XmaI-BamHI-
FT                   XbaI-AccI-HincII-SalI-BspMI-PstI-HindIII"
FT   CDS             complement(>488..<1464)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    546..546
FT                   /note="SIT AatI"
FT   misc_binding    662..662
FT                   /note="SIT SspI"
FT   misc_binding    942..942
FT                   /note="SIT BcgI"
FT   misc_binding    986..986
FT                   /note="SIT ScaI"
FT   misc_binding    1244..1244
FT                   /note="SIT FspI"
FT   misc_binding    1384..1384
FT                   /note="SIT Cfr101I"
FT   misc_binding    1943..1943
FT                   /note="SIT AlwNI"
FT   misc_binding    2357..2357
FT                   /note="SIT AflIII"
FT   misc_binding    2383..2383
FT                   /note="SIT TfiI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2607..2607
FT                   /note="SIT BsaAI"
FT   misc_binding    2612..2612
FT                   /note="SIT Tth111I"
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SQ   Sequence 2780 BP; 687 A; 699 C; 728 G; 666 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggcggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     ttcgccattc aggctacgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     acgccagctg gcgaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgggctgc aggtcgactc
     tagaggatcc ccgggcgagc tcgaattccg gtctccctat agtgagtcgt attaatttcg
     ataagccagc tgggcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     tatcacgagg ccctttcgtc
//