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ID   PT72       preliminary; circular DNA; SYN; 2777 BP.
XX
AC   IG1125;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7-2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGP1-1 from pKC30 & T7 phage gene I & linker
RC   pGP1-2 from pGP1-1 & pKB280-cI857 & pACYC177
RC   from pGP1-2 & pT7-1
RC   pGP5-1 from pT7-1 & T7 genes 5/5.3
RC   from pGP1-2 & pGP5-1
RC   [pT7-0, pT7-1, pT7-2 from T7]
RA   Tabor S., Richardson C.C.;
RT   "A bacteriophage T7 RNA polymerase/promoter system for controlled
RT   exclusive expression of specific genes";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:1074-1078(1985).
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CC   Transcription begins at 2324 and goes rightward.
CC   AMP 201..1061.
CC   MCS 2333..2383 follows T7 promoter.
CC   2147..2284 and 2643..2780 repeat.
CC   NM (pT7-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)
CC   SE ()
CC   PA (pBR322)
CC   BR (pT7-0)(pT7-1)(pT7-7)(pT7-SC)(pT7-SCII)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. T7, promoter
FT                   -> pT7-2 2777bp"
FT   misc_binding    395..440
FT                   /note="MCS EcoRI-BanII-SacI-AvaI-SmaI-XmaI-BamHI-
FT                   XbaI-AccI-HincII-SalI-BspMI-PstI-HindIII"
FT   promoter        complement(440..440)
FT                   /note="PRO bacteriophage T7"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1870..1870
FT                   /note="SIT Cfr101I"
FT   misc_binding    2010..2010
FT                   /note="SIT FspI"
FT   misc_binding    2268..2268
FT                   /note="SIT ScaI"
FT   misc_binding    2306..2306
FT                   /note="SIT BcgI"
FT   misc_binding    2592..2592
FT                   /note="SIT SspI"
XX
SQ   Sequence 2777 BP; 687 A; 699 C; 725 G; 666 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggcggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     ttcgccattc aggctacgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     acgccagctg gcgaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcgccc ggggatcctc
     tagagtcgac ctgcagccca agcttgggtc tccctatagt gagtcgtatt aatttcgata
     agccagctgg gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     ggagtgtata tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtc
//