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ID   PT7SP6     preliminary; circular DNA; SYN; 3100 BP.
XX
AC   IG7044;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7SP6 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR1.1A from pBR322 & T7
RC   pT7SP6 from pT7-1 & pSP64
RA   Axelrod V.D., Kramer F.R.;
RT   "Transcription from bacteriophage T7 and SP6 RNA polymerase
RT   promoters in the presence of 3'-deoxyribonucleoside
RT   5'-triphosphate chain terminators";
RL   Biochemistry 24:5716-5723(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pT7SP6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription both strands)
CC   SE ()
CC   PA (pSP64)(pT7-1)
CC   BR (pBR1.1A from pBR322 and T7)
CC   OF ()
CC   OR (yes)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pT7-1 EcoRI-ScaI 1830bp 444..2274,
FT                   \ phi10 T7 promoter/ori/amp
FT                   2. pSP64 ScaI-EcoRI 1270bp 1782..2999..53,
FT                   \ Sp6 prom/MCS/amp gene frag
FT                   -> pT7SP6 3100bp [3000bp]"
FT   -               1..1270
FT                   /note="pSP64 1782..2999..52 1270bp
FT                   EcoRI = G^AATTC"
FT   -               1271..3100
FT                   /note="pT7-1 444..2273 1830bp
FT                   ScaI = AGT^ACT"
FT   misc_feature    0..0
FT                   /note="MCS HindIII-PstI-SalI-AccI-HincII-XbaI-BamHI-
FT                   AvaI-SmaI-SacI-EcoRI, from pSP64"
FT   misc_feature    0..0
FT                   /note="pT7-1 EcoRI-ScaI"
FT   misc_feature    0..0
FT                   /note="PRO phage phi10 T7 promoter, from pT7-1"
FT   misc_feature    complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   from pT7-1"
FT   misc_feature    complement(0..0)
FT                   /note="ANT E. coli amp gene, from pT7-1 and pSP64"
FT   misc_feature    0..0
FT                   /note="SIT ScaI"
FT   misc_feature    0..0
FT                   /note="pSP64 ScaI-EcoRI"
FT   misc_feature    0..0
FT                   /note="PRO phage SP6 promoter, from pSP64"
XX
SQ   Sequence 3100 BP; 767 A; 797 C; 804 G; 732 T; 0 other;
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatcgacgc tctcccttat gcgactcctg
     cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     ggcgtagagg atctggctag cgatgaccct gctgattggt tcgctgacca tttccggggt
     gcggaacggc gttaccagaa actcagaagg ttcgtccaac caaaccgact ctgacggcag
     tttacgagag agatgatagg gtctgcttca gtaagccaga tgctacacaa ttaggcttgt
     acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
     gatttaggtg acactataga atacaagctt gggctgcagg tcgactctag aggatccccg
     ggcgagctcg aattccggtc tccctatagt gagtcgtatt aatttcgata agccagctgg
     gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt
//