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ID   PT7T318D   preliminary; circular DNA; SYN; 2907 BP.
XX
AC   IG0336;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7T3-18D - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pT7T3-18D from pT7T3-18U & pUC18
RA   ;
RT   ;
RL   Unpublished (1992).
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CC   NM (pT7T3-18D)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli NM522)
CC   CP ()
CC   FN (cloning cDNA)(cloning directional)(transcription)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pT7T3-18U)(pUC18)(bacteriophage f1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18, lacZ gene/f1 ori
FT                   2. pT7T3-18U 2883bp, ori/amp gene/T3/T7
FT                   -> pT7T3-18D 2907bp"
FT   CDS             complement(1..<472)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 universal"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    195..258
FT                   /note="MCS unique HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT                   NotI-EcoRI-SfiI-XhoI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2021..2021
FT                   /note="SIT unique ScaI"
FT   misc_binding    2138..2138
FT                   /note="SIT unique XmnI"
FT   rep_origin      <2777..2907
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2777..2777
FT                   /note="SIT unique NaeI"
XX
SQ   Sequence 2907 BP; 750 A; 718 C; 726 G; 713 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctatat aaattaaccc
     tcactaaagg gaataagctt gcatgcctgc aggtcgactc tagaggatcc gcggccgcgg
     gtaccgagct cgaattcggc ctcgagggcc aattccctat agtgagtcgt attaaattcg
     taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     gaaattgtaa acgttaatgt tttgttaaat ttcgcgttaa atatttgtta aatcagctta
     ttttttaacc agtaagcaga aaatgacaaa aatccttata aatcaaaaga atagaccgag
     ttagttgtga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     aacgtaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt
     tttggaggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     cgcttaatgc gccgctactg ggcgcgt
//