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ID   PT7T319    preliminary; circular DNA; SYN; 2238 BP.
XX
AC   L08952; VB0118; IG0141;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7T3-19 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2238
RC   pT7T3-19
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   old BRL vector.
CC   NM (pT7T3-19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC19)(PromT7)(PromT3)
CC   BR (pT7T3-18)
CC   OF (pT7T3alpha-19)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19c 2686bp, ori/amp gene
FT                   2. pBR322
FT                   3. T3, promoter
FT                   4. T7, promoter
FT                   -> pT7T3-19 2238bp"
FT   promoter        41..60
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    complement(75..131)
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-
FT                   SphI-HindIII; M13mp18/pUC18-polylinker"
FT   promoter        complement(138..157)
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    168..2228
FT                   /note="from pUC19c"
FT   misc_feature    222..2236
FT                   /note="from pBR322"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    complement(1171..1959)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2238 BP; 572 A; 560 C; 533 G; 573 T; 0 other;
     aattctcatg tttgacagct tatcatcgac tccagtttct aattaaccct cactaaaggg
     agagaccata gatggaattc gagctcggta cccggggatc ctctagagtc gacctgcagg
     catgcaagct tgggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta
     atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     ctttcgtctt caagaatt
//