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ID   PT7T3A18   preliminary; circular DNA; SYN; 2887 BP.
XX
AC   IG0142; L08953; VB0119;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7T3alpha-18 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   from pUC18, wild-type; [Pharmacia]
RC   from pUC18, mutant;[BRL,USB,Boehringer;deletes C in 2nd codon of lacZ]
RA   Lobet Y., Peacock M.G., Cieplak W.;
RT   "Frame-shift mutation in the lacZ gene of certain commercially
RT   available pUC18 plasmids";
RL   Nucleic Acids Res. 17:4897-4897(1989).
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RN   [2]
RP   1-2887
RC   pT7T3A18
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
CC   size is 2886 for BRL
CC   GenBank entry is not current with BRL entry (1993).
CC   Contains the almost silent mutation in lacZ.
CC   NM (pT7T3alpha-18)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli DH5alphaF')(E.coli DH5alpha)(E.coli JM101)
CC   CP ()
CC   FN (sequencing)(cloning)(in vitro transcription)
CC   SE ()
CC   PA (pUC18)(pT7T3-18)
CC   BR (pT7T3alpha-19)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18, lacZ gene/f1 ori
FT                   2. pT7T3-18 2239bp, ori/amp gene/T3/T7
FT                   -> pT7T3alpha-18 2887bp"
FT   CDS             complement(1..234)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   primer_bind     125..147
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     140..156
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        164..183
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    187..238
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        243..262
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    621..621
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(>621..<723)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1032..1032
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(1626..2486)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1992..1992
FT                   /note="SIT unique ScaI"
FT   misc_binding    2030..2030
FT                   /note="SIT unique BcgI"
FT   misc_binding    2050..2050
FT                   /note="SIT unique AcyI"
FT   misc_binding    2109..2109
FT                   /note="SIT unique XmnI"
FT   misc_binding    2346..2346
FT                   /note="SIT unique BsmAI"
FT   rep_origin      complement(>2349..<2890)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2559..2559
FT                   /note="SIT unique NaeI"
FT   misc_binding    2662..2662
FT                   /note="SIT unique DraII"
XX
SQ   Sequence 2887 BP; 701 A; 736 C; 705 G; 745 T; 0 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctaaccct cactaaaggg
     agagacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     ttccctatag tgagtcgtat taaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctgg acgcgccctg tagcggcgca ttaagcgcgg
     cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     caatttc
//