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ID   PT7T3A19   preliminary; circular DNA; SYN; 2890 BP.
XX
AC   IG0143; L08954; VB0120;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pT7T3alpha-19 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2890
RC   pT7T3A19
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   GenBank entry is not current with BRL entry (1993).
CC   NM (pT7T3alpha-19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli DH5alphaF')(E.coli DH5alpha)(E.coli JM101)
CC   CP ()
CC   FN (sequencing)(cloning)(in vitro transcription)
CC   SE ()
CC   PA (pUC19)(pT7T3-19)
CC   BR (pT7T3alpha-18)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19, lacZ gene/f1 ori
FT                   2. pT7T3-19 2238bp, ori/amp gene/T3/T7
FT                   -> pT7T3alpha-19 2890bp"
FT   CDS             complement(1..234)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   primer_bind     125..147
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     140..156
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        164..183
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    184..235
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   BspMI-PstI-SphI-HindIII"
FT   promoter        243..262
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    621..621
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(>621..<723)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1032..1032
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(1626..2486)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1992..1992
FT                   /note="SIT unique ScaI"
FT   misc_binding    2030..2030
FT                   /note="SIT unique BcgI"
FT   misc_binding    2050..2050
FT                   /note="SIT unique AcyI"
FT   misc_binding    2109..2109
FT                   /note="SIT unique XmnI"
FT   misc_binding    2346..2346
FT                   /note="SIT unique BsmAI"
FT   rep_origin      complement(>2349..<2890)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2559..2559
FT                   /note="SIT unique NaeI"
FT   misc_binding    2662..2662
FT                   /note="SIT unique DraII"
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SQ   Sequence 2890 BP; 700 A; 734 C; 709 G; 747 T; 0 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgaat taaccctcac taaagggaga
     gacgaattcg agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt
     tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     tccgcgcaca tttccccgaa aagtgccacc tggacgcgcc ctgtagcggc gcattaagcg
     cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     ttacaatttc
//