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ID   PTCF       preliminary; circular DNA; SYN; 7941 BP.
XX
AC   L19899;
XX
DT   24-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pTCF - complete.
XX
KW   cloning vector; beta-lactamase.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7941
RC   pTCF from lambda, cos
RA   Bodenteich A., Chissoe S.L., Wang Z., Roe B.A.;
RT   "Nucleotide sequence of three cosmid cloning vectors: svPHEP, pTCF,
RT   and LAWRIST16";
RL   Unpublished (1993).
XX
CC   A cosmid cloning vector constructed by F. Grosveld, Department of
CC   Cell Biology, Erasmus University, Rotterdam, Netherlands.
CC   NCBI gi: 310738
CC   NM (pTCF)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 3600bp, cos
FT                   2. pBR322 4361bp, ori/amp gene
FT                   -> pTCF 7941bp"
FT   CDS             1559..2419
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp);
FT                   9 stops when translation attempted, frame 1, code 0"
XX
SQ   Sequence 7941 BP; 1736 A; 2285 C; 2133 G; 1787 T; 0 other;
     gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
     ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
     cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
     ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
     ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tcccatagtt
     gcctgcaact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt cccgcccgta
     gcgcgcgcgc accagccggc atcgcacccg agcaccagct cccccgtcgt ccagatgccc
     acgggccacg tcgaggccga cggggagaaa tacacgtacc tacctgggga tctcaacagg
     ccccgggtgg ccaaccaggt cgtggacgcg ttgtgcaggt gcgtgatgtc cagctccgtc
     gtcgggtgcc gccgggcccc aaccggcggt cgggggggcg gtgtatcacg cggcccgctc
     gggtggctcg ccgtcgccac gttgtctccc cgcgggaacg tcagggcctc ggggtcaggg
     acggccgaaa acgttaccca ggcccgggaa cgcagcaaca cggaggcggc tggattgtgc
     aagagaccct taaggggggc gaccgagggg ggaggctggg cggtcggctc gaccgtggtg
     ggggcgggca ggctcgcgtt cgggggccgg ccgagcaggt aggtcttcgg gatgtaaagc
     agctggccgg ggtcccgcgg aaactcggcc gtggtgacca atacaaaaca aaagcgctcc
     tcgtaccagc gaagaagggg cagagatgcc gtagtcaggt ttagttcgtc cggcggcgcc
     agaaatccgc gcggtggttt ttgggggtcg ggggtgtttg gcagccacag acgcccggtg
     ttcgtgtcgc gccagtacat gcggtccatg cccaggccat ccaaaaacca tgggtctgtc
     tgctcagtcc agtcgtggac ctgaccccac gcaacgccca aaataataac ccccacgaac
     cataaaccat tccccatggg ggaccccgtc cctaacccac ggggcccgtg gctatggcag
     ggcttgccgc cccgacgttg gctgcgagcc ctgggccttc acccgaactt ggggggtggg
     gtggggaaaa ggaagaaacg cgggcgtatt ggccccaatg gggtctcggt ggggtatcga
     cagagtgcca gccctgggac cgaaccccgc gtttatgaac aaacgaccca acaccgtgcg
     ttttattctg tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc
     gtgtttcagt tagcctcccc catctcccgg ggtgggcgaa gaactccagc atgagatccc
     cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     ttgatcagat ctgcggcacg ctgttgacgc tgttaagcgg gtcgctgcag ggtcgctcgg
     tattcgaggc cacacgcgtc accttaatat gcgaagtgga cctgggaccg cgccgccccg
     actgcatctg cgtgttcgaa ttcgccaatg acaagacgct gggcggggtt tgtgtcatca
     tagaactaaa gacatgcaaa tatatttctt ccggggacac cgccagcaaa cgcgagcaac
     gggccacggg gatgaagcag ctgcgccact ccctgaagct cctgcagtcc ctcgcgcctc
     cgggtgacaa gatagtgtac ctgtgccccg tcctggtgtt tgtcgcccaa cggacgctcc
     gcgtcagccg cgtgacccgg ctcgtcccgc agaaggtctc cggtaatatc accgcagtcg
     tgcggatgct ccagagcctg tccacgtata cggtccccat tgagcctagg acccagcgag
     cccgtcgccg ccgcggcggc gccgcccggg ggtctgcgag cagaccgaaa aggtcacact
     ctggggcgcg cgacccgccc gagtcagcgg cccgccagtt accacccgcc gaccaaaccc
     ccgcctccac ggagggcggg ggggtgctta agaggatcgc ggcgctcttc tgcgtgcccg
     tggccaccaa gaccaaaccc cgagccgcct ccgaatgaga gtgtttcgtt ccttccccct
     ccccccgcgt cagacaaacc ctaaccaccg cttaagcggc ccccgcgagg tccgaagact
     catttggatc gatccggaat tctcatgttt gacagcttat catcgataag ctttaatgcg
     gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     actacgcgat catggcgacc acacccgtcc tgtggatctg cctcgttggc ctgccgcagt
     tcttcaacct cccggcgcag cttttcgttc tcaatttcag catccctttc ggcataccat
     tttatgacgg cggcagagtc ataaagcacc tcattaccct tgccaccgcc tcgcagaacg
     ggcattccct gttcctgcca gttctgaatg gtacggatac tcgcaccgaa aatgtcagcc
     agctgctttt tgttgacttc cattgttcat tccacggaca aaaacagaga aaggaaacga
     cagaggccaa aaagctcgct ttcagcacct gtcgtttcct ttcttttcag agggtatttt
     aaataaaaac attaagttat gacgaagaag aacggaaacg ccttaaaccg gaaaattttc
     ataaatagcg aaaacccgcg aggtcgccgc cccgtaacaa ggcggatcgc cggaaaggac
     ccgcaaatga taataattat caattgcata ctatcgacgg cactgctgcc agataacacc
     accggggaaa cattccatca tgatggccgt gcggacatag gaagccagtt catccatcgc
     tttcttgtct gctgccattt gctttgtgac atccagcgcc gcacattcag cagcgttttt
     cagcgcgttt tcgatcaacg tttcaatgtt ggtatcaaca ccaggtttaa ctttgaactt
     atcggcactg acggttacct tgttctgcgc tggctcatca cgctggatac caaggctgat
     gttgtagata ttggtcaccg gctgaggtgt ttcgattgcc gctgcgtgga tagcaccatt
     tgcgatagcg gcgtccttga tgaatgacac tccattgcga ataagttcga aggagacggt
     gtcacgaatg cgctggtcca gctcgtcgat tgccttttgt gcagcagagg tatcaatctc
     aacgccaagc gtcatcgaag cgcaatattg ctgctcacca aaacgcgtat tgaccaggtg
     ttcaacggca aatttctgcc cttctgatgt cagaaaggta aagtgatttt ctttctggta
     ttcagttgct gtgtgtctgg tttcagcaaa accaagctcg cgcaattcgg ctgtgccaga
     tttagaaggc agatcaccag acagcaacgc gccacggaaa aacagcgcat acagaacatc
     cgtcgccgcg ccggacaacg tgataatttt atgacccatg atttatttcc ttttagacgt
     gagcctgtcg cacagcaaag ccgccgaaag ttaacggttt gcccaggctc acaactgaaa
     gactttctac ggtgtgcgcg tgcgatgcgc gtagaagact gatttatcaa cctgtcttta
     tatcaggatt cattacctga ctatttgtgg gtaaagttcg tagtgcgctg atcgtgcaaa
     atgattttag ttgggaacag ttcgcaactc tgtcccataa aaatcagcat attcccatct
     atcccatatc cagcgcattg accatcggga tactgaaggg agattccatc atctcttaga
     aagatcacca tctcttttgt ttcaatttgc atatagctac ctggaggatt tatgaataca
     aggattttca tggactatta ccatgagatt gattttccat ctttattcgc gagagcagtg
     gaaagcgatg acgatgtggg tactacattg cgcattcacc tactttgtga gcgcatggtc
     gaagcatgga tatgcgcatg ctgtgactgc caagatcctc tacgccggac gcatcgtggc
     cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     gggagagcgt cgacgggatc c
//