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ID   PTF1       preliminary; circular DNA; SYN; 3043 BP.
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AC   S52444; ATCC77397;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli phagemid vector pTF1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   plasmid from pTZ18R & pAR2529 or pET-3a
RC   plasmid2 from plasmid
RC   pTF1 from plasmid2 & pBluescript KS+
RA   Takahashi T., Ryan K.W., Portner A.;
RT   "A plasmid that improves the efficiency of foreign gene expression
RT   by intracellular T7 RNA polymerase";
RL   Gene Anal. Tech. 9:91-95(1992).
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CC   pTF1 requires a reverse primer.
CC   This entry [NCBI gibbsq 122487] was created by the journal scanning
CC   component of NCBI/GenBank at the National Library of Medicine.
CC   This sequence comes from Fig. 1C.
CC   Deposited by: Allen Portner
CC   High-copy-number plasmid (in bacteria) with increased efficiency of
CC   liposome-mediated introduction into cultured cells. [1]
CC   Can express foreign sequences in cells producing T7 RNA polymerase,
CC   such as that encoded by recombinant vaccinia virus vTF7-3
CC   ATCC VR-2153. [1]
CC   Constructed by inserting the 152 bp BamHI-BglII fragment from pAR2529
CC   into the BamHI site of pTZ18R.  Polylinker sites were removed and were
CC   replaced with the 48 bp EcoRI-KpnI fragment of pBluescript KS+. [1]
CC   The order of the major features of the plasmid is: universal primer
CC   sequence - XbaI - T7 phi 10 terminator < - XbaI - BamHI/MCS/EcoRI -
CC   < T7 phi 10 promoter - reverse primer sequence - pMB1 ori - < ampR -
CC   f1 ori.[1]CC   A correction to the published sequence has been
CC   submitted.
CC   Sequence 2173-2252 should read:
CC   GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT
CC   ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--3.0; EcoRI--3.0; HindIII--3.0. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   Sequence comes from Fig. 1C. [1]
CC   NCBI gi: 263065
CC   NM (pTF1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM103)(E.coli NM522)(E.coli DH5alpha)
CC   HO (vertebrate cells)(HeLa T4+ cells)
CC   CP ()
CC   FN (cloning)(sequencing)(mutagenesis)(transcription)
CC   SE (color blue/white)
CC   PA (pTZ18R)(pAR2529)(pBluescript II KS+)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ18R BamHI 2861bp 193..193
FT                   2. pET-3a BamHI-BglII 152bp, T7 terminator
FT                   -> plasmid 3013bp
FT                   1. plasmid remove HindIII-HincII 20bp 163..183,
FT                   \ 3' to T7 term/2993bp [18bp]
FT                   Klenow:Klenow
FT                   -> plasmid2 2993bp
FT                   1. plasmid2 remove EcoRI-KpnI 8bp 206..214, MCS
FT                   \ 2985bp [12bp]
FT                   2. pBluescript KS+ EcoRI-KpnI 52bp 708..760,
FT                   \ MCS [48bp]
FT                   -> pTF1 3043bp"
FT   misc_binding    1..1
FT                   /note="SIT BglI"
FT   terminator      181..318
FT                   /note="TER bacteriophage T7 transcription termination,
FT                   from Tphi"
FT   misc_binding    327..390
FT                   /note="MCS unique BamHI-SmaI-KpnI-ApaI-XhoI-SalI-
FT                   AccI-HincII-ClaI-HindIII-EcoRV-EcoRI"
FT   promoter        complement(392..411)
FT                   /note="PRO bacteriophage T7, from phi10"
FT   misc_binding    0..0
FT                   /note="SIT unique AflI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique AhaII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   rep_origin      0..3043
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    0..0
FT                   /note="SIT unique NaeI"
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SQ   Sequence 3043 BP; 734 A; 774 C; 736 G; 799 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgact ctagaggatc
     tcgatccgga tatagttcct cctttcagca aaaaacccct caagacccgt ttagaggccc
     caaggggtta tgctagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg
     ctttgttagc agccggatga ctctagagga tccccgggta ccgggccccc cctcgaggtc
     gacggtatcg ataagcttga tatcgaattc cctatagtga gtcgtattaa attcgtaatc
     atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     atagtgtatg cgcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     tcaaccaagt cattctgaga atagtgtatg cggaaaacgt tcttcggggc gaaaactctc
     aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     ttagaaaaat aaacaaatag gggttcctct cacatttccc cgaaaagtgc cacctgacgc
     gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc gatttagtgc
     tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     gattttgccg atttcggcct attggttaaa aaatgtgctg atttaacaaa aatttaacgc
     gaattttaac aaaatattaa caaaatatta acgtttacaa ttt
//