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ID   PTG190     preliminary; circular DNA; SYN; 2970 BP.
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AC   IG9944;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector ptg190 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   ptg190 from pML2 & pUC8
RC   ptg191 from ptg190
RC   pPolyI or ptg1poly from ptg191 & M13tg131
RC   pPolyI+ from pPolyI & linker
RC   pPolyI+B0 from pPolyI+ & linker
RC   pPolyII from pPolyI+B0 & oligo
RC   M13-sn-19 from M13mp18 & oligo
RC   pPolyII-sn-14 from M13-sn-19 & pPolyII
RC   pPolyIII from pPolyII & M13-sn-19
RC   pPolyIII-I, pPolyIII-D from pPolyII-sn-14 & M13-sn-19
RC   pPolyIII-I-AMP, pPolyIII-I-ORI from M13tg119 & pPolyIII-I
RC   pPolyIII-D-AMP, pPolyIII-D-ORI from M13tg119 & pPolyIII-D
RC   EMBL301 or ABRO1, EMBL302 from EMBL3 & M13-sn-19
RA   Lathe R., Vilotte J.L., Clark A.J.;
RT   "Plasmid and bacteriophage vectors for excision of intact
RT   inserts";
RL   Gene 57:193-201(1987).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (ptg190)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pML2)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pML2 remove DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1772..2483, amp gene with no PstI/2189bp
FT                   2. pUC8 DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1074..1785, amp gene with PstI
FT                   -> ptg190 2900bp"
FT   -               1..1771
FT                   /note="pML2 1..1771 1771bp
FT                   AhaIII = DraI = TTT^AAA"
FT   -               1772..2482
FT                   /note="pUC8 1074..1784 711bp
FT                   AhaIII = DraI = TTT^AAA"
FT   -               2483..2970
FT                   /note="pML2 2483..2970 488bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2970 BP; 690 A; 792 C; 766 G; 722 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaagttctg ctatgtggcg cggtattatc
     ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     tttaaaactt catttttaat ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc
     ggtagtttat cacagttaaa ttgctaacgc
//