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ID   PTG191     preliminary; circular DNA; SYN; 2978 BP.
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AC   IG9945;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector ptg191 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   ptg190 from pML2 & pUC8
RC   ptg191 from ptg190
RC   pPolyI or ptg1poly from ptg191 & M13tg131
RC   pPolyI+ from pPolyI & linker
RC   pPolyI+B0 from pPolyI+ & linker
RC   pPolyII from pPolyI+B0 & oligo
RC   M13-sn-19 from M13mp18 & oligo
RC   pPolyII-sn-14 from M13-sn-19 & pPolyII
RC   pPolyIII from pPolyII & M13-sn-19
RC   pPolyIII-I, pPolyIII-D from pPolyII-sn-14 & M13-sn-19
RC   pPolyIII-I-AMP, pPolyIII-I-ORI from M13tg119 & pPolyIII-I
RC   pPolyIII-D-AMP, pPolyIII-D-ORI from M13tg119 & pPolyIII-D
RC   EMBL301 or ABRO1, EMBL302 from EMBL3 & M13-sn-19
RA   Lathe R., Vilotte J.L., Clark A.J.;
RT   "Plasmid and bacteriophage vectors for excision of intact
RT   inserts";
RL   Gene 57:193-201(1987).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (ptg191)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pML2)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pML2 remove DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1772..2483, amp gene with no PstI/2189bp
FT                   2. pUC8 DraI/AhaIII-DraI/AhaIII 711bp
FT                   \ 1074..1785, amp gene with PstI
FT                   -> ptg190 2771bp
FT                   1. ptg190 NruI 2771bp 905..905
FT                   BglII linker 8bp cagatctg
FT                   -> ptg191 2779bp"
FT   -               1..904
FT                   /note="pML2 1..904 904bp
FT                   NruI = TCG^CGA
FT                   \          cagatctg"
FT   -               905..912
FT                   /note="cagatctg 8bp
FT                   \ cagatctg
FT                   NruI = TCG^CGA"
FT   -               913..1779
FT                   /note="pML2 905..1771 867bp
FT                   AhaIII = DraI = TTT^AAA"
FT   -               1780..2490
FT                   /note="pUC8 1074..1784 711bp
FT                   AhaIII = DraI = TTT^AAA"
FT   -               2491..2978
FT                   /note="pML2 2483..2970 488bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2978 BP; 692 A; 794 C; 768 G; 724 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcagatc tgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     catgagatta tcaaaaagga tcttcaccta gatcctttta aagttctgct atgtggcgcg
     gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     tagattgatt taaaacttca tttttaattt aaaagtgctc atcattggaa aacgttcttc
     ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc atcgataagc
     tttaatgcgg tagtttatca cagttaaatt gctaacgc
//