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ID PTK2 preliminary; circular DNA; SYN; 7940 BP.
XX
AC IG9844;
XX
DT 01-JUL-1995 (Rel. 11, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE Vertebrate/E.coli plasmid vector pTK2 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSKS103 from pBR322 & pTK2
RC pSKS108 from pSKS105 & pSKS103
RC pSKS116 from pSKS108
RC pSKS111 from pSKS116 & pSKS106, lacZgene
RC pMC1033 from Tn3 tnpR gene
RC pSKS121 from pSKS116 & pMC1033
RC pSKS114 from pSKS116 & Tn9 IS1 & pTK2
RC pSKS122 from pSKS116 & pSKS114
RA Shapira S.K., Casadaban M.J.;
RT "Hybrid protein thymidine kinase gene fusions: plasmid vectors for
RT the study of transcription and translation initiation signals";
RL Gene 52:83-94(1987).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pTK2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli HB101)(vertebrate cells)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(HSV-1)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 BamHI 4361bp 376..376
FT 2. HSV-1 BamHI-BamHI 3579bp 45056..48635,
FT \ Q frag tk gene/.28 to .32
FT \ [tk gene CDS = #X14112 46672..47802]
FT -> pTK2 7940bp [7800bp]"
FT - 1..375
FT /note="pBR322 1..375 375bp
FT BamHI = G^GATCC"
FT - 376..3954
FT /note="HSV-1 45056..48635 3579bp
FT BamHI = G^GATCC"
FT - 3955..7940
FT /note="pBR322 376..4361 3986bp"
FT misc_binding 0..0
FT /note="MCS unique EcoRI"
FT misc_binding 0..0
FT /note="MCS unique BamHI"
FT CDS 0..0
FT /note="GEN vertebrate thymidine kinase gene (TK);
FT truncated"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
XX
SQ Sequence 7940 BP; 1633 A; 2360 C; 2328 G; 1619 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc aagactgaca cattaaaaaa cacagaatcc gcggcacagc
ccgcggcccc gcgggcggcc aacccggcaa gcgcgcgcga gtgggccaaa aagcctagca
ggtcggagag gcagaccgcg ccgtttgcgt gggcggcgtt cacgaaagca aaacccgacg
tcgcgagcag ccccgttagg cgccagaaga gaggggggcg cgggccctgc tcggcgcccg
cgtcccccga gaaaaactcc gcgtatgccc gcgacaggaa ctgggcgtag ttcgtgccct
cctccgggta gccgcccacg cggcggaggg cgtccagcgc ggagccgttg tcggcccgcg
tcagggaccc taggacaaag acccgatacc gggggccgcc cgggggcccg ggaagagccc
ccggggggtt ttcgtccgcg gggtccccga cccgatctag cgtctggccc gcggggacca
ccatcacttc caccggaggg ctgtcgtgca tggatatcac gagccccatg aattcccgcc
cgtagcgcgc gcgcaccagc gcggcatcgc acccgagcac cagctccccc gtcgtccaga
tgcccacggg ccacgtcgag gccgacgggg agaaatacac gtacctacct ggggatctca
acaggccccg ggtggccaac caggtcgtgg acgcgttgtg caggtgcgtg atgtccagct
ccgtcgtcgg gtgccgccgg gccccaaccg gcggtcgggg gggcggtgta tcacgcggcc
cgctcgggtg gctcgccgtc gccacgttgt ctccccgcgg gaacgtcagg gcctcggggt
cagggacggc cgaaaacgtt acccaggccc gggaacgcag caacacggag gcggctggat
tgtgcaagag acccttaagg ggggcgaccg aggggggagg ctgggcggtc ggctcgaccg
tggtgggggc gggcaggctc gcgttcgggg gccggccgag caggtaggtc ttcgggatgt
aaagcagctg gccggggtcc cgcggaaact cggccgtggt gaccaataca aaacaaaagc
gctcctcgta ccagcgaaga aggggcagag atgccgtagt caggtttagt tcgtccggcg
gcgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc cacagacgcc
cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa aaccatgggt
ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata ataaccccca
cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc ccgtggctat
ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
ccttccgtgt ttcagttagc ctcccccatc tcccgggcaa acgtgcgcgc caggtcgcag
atcgtcggta tggagcctgg ggtggtgacg tgggtctgga ccatcccgga ggtaagttgc
agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa gacatgcatg
ggacggaggc gtttggccaa gacgtccaaa gcccaggcaa acacgttata caggtcgccg
ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc gatatggggt
cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggccgc ccccgaaagc
tgtccccaat cctcccgcca cgacccgccg ccctgcagat accgcaccgt attggcaagc
agcccataaa cgcggcgaat cgcggccagc atagccaggt caagccgctc gccggggcgc
tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggcccccaa cacgatgttt
gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg gggggtcatg
ctgcccataa ggtatcgcgc ggccgggtag cacaggaggg cggcgatggg atggcggtcg
aagatgaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc cccgatatga
ggagccagaa cggcgtcggt cacggcataa ggcatgccca ttgttatctg ggcgcttgtc
attaccaccg ccgcgtcccc ggccgatatc tcaccctggt cgaggcggtg ttgtgtggtg
tagatgttcg cgattgtctc ggaagccccc aacacccgcc agtaagtcat cggctcgggt
acgtagacga tatcgtcgcg cgaacccagg gccaccagca gttgcgtggt ggtggttttc
cccatcccgt ggggaccgtc tatataaacc cgcagtagcg tgggcatttt ctgctccagg
cggacttccg tggctttttg ttgccggcga gggcgcaacg ccgtacgtcg gttgttatgg
ccgcgagaac gcgcagcctg gtcgaacgca gacgcgtgtt gatggcaggg gtacgaagcc
atacgcgctt ctacaaggcg ctggccgaag aggtgcggga gtttcacgcc accaagatct
gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggta ttcgaggcca
cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg ccgccccgac tgcatctgcg
tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga
catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga
tgaagcagct gcgccactcc ctgaagctcc tgcagtccct cgcgcctccg ggtgacaaga
tagtgtacct gtgccccgtc ctggtgtttg tcgcccaacg gacgctccgc gtcagccgcg
tgacccggct cgtcccgcag aaggtctccg gtaatatcac cgcagtcgtg cggatgctcc
agagcctgtc cacgtatacg gtccccattg agcctaggac ccagcgagcc cgtcgccgcc
gcggcggcgc cgcccggggg tctgcgagca gaccgaaaag gtcacactct ggggcgcgcg
acccgcccga gtcagcggcc cgccagttac cacccgccga ccaaaccccc acctccacgg
agggcggggg ggtgcttaag aggatcgcgg cgctcttctg cgtgcccgtg gccaccaaga
ccaaaccccg agccgcctcc gaatgagagt gtttcgttcc ttccccctcc ccccgcgtca
gacaaaccct aaccaccgct taagcggccc ccgcgaggtc cgaagactca tttggatcct
ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
ggccctttcg tcttcaagaa
//