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ID   PTK2       preliminary; circular DNA; SYN; 7940 BP.
XX
AC   IG9844;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pTK2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSKS103 from pBR322 & pTK2
RC   pSKS108 from pSKS105 & pSKS103
RC   pSKS116 from pSKS108
RC   pSKS111 from pSKS116 & pSKS106, lacZgene
RC   pMC1033 from Tn3 tnpR gene
RC   pSKS121 from pSKS116 & pMC1033
RC   pSKS114 from pSKS116 & Tn9 IS1 & pTK2
RC   pSKS122 from pSKS116 & pSKS114
RA   Shapira S.K., Casadaban M.J.;
RT   "Hybrid protein thymidine kinase gene fusions: plasmid vectors for
RT   the study of transcription and translation initiation signals";
RL   Gene 52:83-94(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTK2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(HSV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. HSV-1 BamHI-BamHI 3579bp 45056..48635,
FT                   \ Q frag tk gene/.28 to .32
FT                   \ [tk gene CDS = #X14112 46672..47802]
FT                   -> pTK2 7940bp [7800bp]"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC"
FT   -               376..3954
FT                   /note="HSV-1 45056..48635 3579bp
FT                   BamHI = G^GATCC"
FT   -               3955..7940
FT                   /note="pBR322 376..4361 3986bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK);
FT                   truncated"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 7940 BP; 1633 A; 2360 C; 2328 G; 1619 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc aagactgaca cattaaaaaa cacagaatcc gcggcacagc
     ccgcggcccc gcgggcggcc aacccggcaa gcgcgcgcga gtgggccaaa aagcctagca
     ggtcggagag gcagaccgcg ccgtttgcgt gggcggcgtt cacgaaagca aaacccgacg
     tcgcgagcag ccccgttagg cgccagaaga gaggggggcg cgggccctgc tcggcgcccg
     cgtcccccga gaaaaactcc gcgtatgccc gcgacaggaa ctgggcgtag ttcgtgccct
     cctccgggta gccgcccacg cggcggaggg cgtccagcgc ggagccgttg tcggcccgcg
     tcagggaccc taggacaaag acccgatacc gggggccgcc cgggggcccg ggaagagccc
     ccggggggtt ttcgtccgcg gggtccccga cccgatctag cgtctggccc gcggggacca
     ccatcacttc caccggaggg ctgtcgtgca tggatatcac gagccccatg aattcccgcc
     cgtagcgcgc gcgcaccagc gcggcatcgc acccgagcac cagctccccc gtcgtccaga
     tgcccacggg ccacgtcgag gccgacgggg agaaatacac gtacctacct ggggatctca
     acaggccccg ggtggccaac caggtcgtgg acgcgttgtg caggtgcgtg atgtccagct
     ccgtcgtcgg gtgccgccgg gccccaaccg gcggtcgggg gggcggtgta tcacgcggcc
     cgctcgggtg gctcgccgtc gccacgttgt ctccccgcgg gaacgtcagg gcctcggggt
     cagggacggc cgaaaacgtt acccaggccc gggaacgcag caacacggag gcggctggat
     tgtgcaagag acccttaagg ggggcgaccg aggggggagg ctgggcggtc ggctcgaccg
     tggtgggggc gggcaggctc gcgttcgggg gccggccgag caggtaggtc ttcgggatgt
     aaagcagctg gccggggtcc cgcggaaact cggccgtggt gaccaataca aaacaaaagc
     gctcctcgta ccagcgaaga aggggcagag atgccgtagt caggtttagt tcgtccggcg
     gcgccagaaa tccgcgcggt ggtttttggg ggtcgggggt gtttggcagc cacagacgcc
     cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag gccatccaaa aaccatgggt
     ctgtctgctc agtccagtcg tggacctgac cccacgcaac gcccaaaata ataaccccca
     cgaaccataa accattcccc atgggggacc ccgtccctaa cccacggggc ccgtggctat
     ggcagggctt gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     ttggggtggg gaaaaggaag aaacgcgggc gtattggtcc caatggggtc tcggtggggt
     atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     cgtgcgtttt attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct
     ccttccgtgt ttcagttagc ctcccccatc tcccgggcaa acgtgcgcgc caggtcgcag
     atcgtcggta tggagcctgg ggtggtgacg tgggtctgga ccatcccgga ggtaagttgc
     agcagggcgt cccggcagcc ggcgggcgat tggtcgtaat ccaggataaa gacatgcatg
     ggacggaggc gtttggccaa gacgtccaaa gcccaggcaa acacgttata caggtcgccg
     ttgggggcca gcaactcggg ggcccgaaac agggtaaata acgtgtcccc gatatggggt
     cgtgggcccg cgttgctctg gggctcggca ccctggggcg gcacggccgc ccccgaaagc
     tgtccccaat cctcccgcca cgacccgccg ccctgcagat accgcaccgt attggcaagc
     agcccataaa cgcggcgaat cgcggccagc atagccaggt caagccgctc gccggggcgc
     tggcgtttgg ccaggcggtc gatgtgtctg tcctccggaa gggcccccaa cacgatgttt
     gtgccgggca aggtcggcgg gatgagggcc acgaacgcca gcacggcctg gggggtcatg
     ctgcccataa ggtatcgcgc ggccgggtag cacaggaggg cggcgatggg atggcggtcg
     aagatgaggg tgagggccgg gggcggggca tgtgagctcc cagcctcccc cccgatatga
     ggagccagaa cggcgtcggt cacggcataa ggcatgccca ttgttatctg ggcgcttgtc
     attaccaccg ccgcgtcccc ggccgatatc tcaccctggt cgaggcggtg ttgtgtggtg
     tagatgttcg cgattgtctc ggaagccccc aacacccgcc agtaagtcat cggctcgggt
     acgtagacga tatcgtcgcg cgaacccagg gccaccagca gttgcgtggt ggtggttttc
     cccatcccgt ggggaccgtc tatataaacc cgcagtagcg tgggcatttt ctgctccagg
     cggacttccg tggctttttg ttgccggcga gggcgcaacg ccgtacgtcg gttgttatgg
     ccgcgagaac gcgcagcctg gtcgaacgca gacgcgtgtt gatggcaggg gtacgaagcc
     atacgcgctt ctacaaggcg ctggccgaag aggtgcggga gtttcacgcc accaagatct
     gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg tcgctcggta ttcgaggcca
     cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg ccgccccgac tgcatctgcg
     tgttcgaatt cgccaatgac aagacgctgg gcggggtttg tgtcatcata gaactaaaga
     catgcaaata tatttcttcc ggggacaccg ccagcaaacg cgagcaacgg gccacgggga
     tgaagcagct gcgccactcc ctgaagctcc tgcagtccct cgcgcctccg ggtgacaaga
     tagtgtacct gtgccccgtc ctggtgtttg tcgcccaacg gacgctccgc gtcagccgcg
     tgacccggct cgtcccgcag aaggtctccg gtaatatcac cgcagtcgtg cggatgctcc
     agagcctgtc cacgtatacg gtccccattg agcctaggac ccagcgagcc cgtcgccgcc
     gcggcggcgc cgcccggggg tctgcgagca gaccgaaaag gtcacactct ggggcgcgcg
     acccgcccga gtcagcggcc cgccagttac cacccgccga ccaaaccccc acctccacgg
     agggcggggg ggtgcttaag aggatcgcgg cgctcttctg cgtgcccgtg gccaccaaga
     ccaaaccccg agccgcctcc gaatgagagt gtttcgttcc ttccccctcc ccccgcgtca
     gacaaaccct aaccaccgct taagcggccc ccgcgaggtc cgaagactca tttggatcct
     ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc
     agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga
     ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca
     cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc
     cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg
     aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc
     gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc
     gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta
     tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct
     tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga
     agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
     cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
     tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg
     atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag
     aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc
     tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag
     tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt
     ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg
     tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt
     tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc
     atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt
     aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg
     gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac
     cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     ggccctttcg tcttcaagaa
//