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ID   PTKBETA    preliminary; circular DNA; SYN; 7481 BP.
XX
AC   IG1130; ATCC77178;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pTKbeta - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pADbeta from adenovirus 2 MLP & SV40 splice/polyA & pUC19
RC   pCMVbeta from CMV IEP & SV40 splice/polyA & pUC19
RC   pSVbeta from SV40 early promoter & SV40 splice/polyA & pUC19
RC   pTKbeta from HSV TK gene promoter & SV40 splice/polyA & pUC19
RA   MacGregor G.R., Caskey C.T.;
RT   "Construction of plasmids that express E. coli beta-galactosidase
RT   in mammalian cells";
RL   Nucleic Acids Res. 17:2365-2365(1989).
XX
RN   [2]
RC   pSV0cat, pSV2cat, pA10CAT2, pCAT..., pBLCAT2, pBLCAT3 from cat gene
RC   pSK-SVCAT, pSK-CAT from cat gene
RC   pRSVL-Adelta5', pSV2AL-Adelta5', pT81luc, pSluc2 from luciferase gene
RC   pXP1, pXP2, pOLUC from luciferase gene
RC   pXGH, pTKGH, pOGH from growth hormone gene
RC   pSV2Apap, pSV232Apap, pSV0Apap from alkaline phosphatase gene
RC   pBC12/RSV/SEAP, pBC12/PL/SEAP from secreted alkaline phosphatase
RC   pCMVbeta, pSVbeta, pNASSbeta from beta-galactosidase gene
RA   Alam J., Cook J.L.;
RT   "Reporter genes: application to the study of mammalian gene
RT   transcription";
RL   Anal. Biochem. 188:245-254(1990).
XX
CC   ATCC size is 7600 bp.
CC   The order of the major features in this plasmid is: pUC19 - EcoRI -
CC   HSV thymidine kinase promoter - SV40 late 16S/19S splice signals -
CC   NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI -
CC   HindIII - pUC19. [1]
CC   The lacZ gene may be excised by NotI and replaced with alternative
CC   sequences. [1]
CC   Reporter plasmid permitting visual detection of beta-galactosidase
CC   activity by histochemical staining. [1]
CC   Designed to express low levels of beta-galactosidase or an
CC   alternative gene product.  May be used as a reference plasmid in
CC   transfection experiments or to monitor the effect of trans-acting
CC   factors. [1]
CC   Restriction digest of the clone give the following sizes (kb):
CC   EcoRI--7.2, 0.9; HindIII--7.6; EcoRI/HindIII--4.5,2.7,0.9;
CC   NotI--4.4, 3.2; BamHI--3.8 (doublet). (ATCC staff)
CC   Medium is -1 TB plus ampicillin (200 ug/ml).
CC   NM (pTKbeta)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(ATCC)
CC   HO (E.coli DH5alpha)(E.coli)(monkey COS cells)(vertebrate cells)
CC   CP ()
CC   FN (expression)(reporter)(promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR (pADbeta)(pNASSbeta)(pCMVbeta)(pSVbeta)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. SV40, splice/polyA
FT                   -> plasmid
FT                   1. plasmid NotI
FT                   2. E. coli NotI-NotI, lacZ gene
FT                   -> plasmid2
FT                   1. plasmid2
FT                   2. HSV, tk promoter/#X14112
FT                   -> pTKbeta 7481bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-XbaI-SalI-PstI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="MCS unique XbaI-NotI-SalI-SphI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   promoter        0..0
FT                   /note="PRO Herpes simplex virus (HSV)
FT                   thymidine kinase (TK) gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO SV40 enhancer"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique NotI"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   misc_binding    0..0
FT                   /note="SIT SacI"
FT   misc_binding    0..0
FT                   /note="SIT NotI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    3900..3900
FT                   /note="SIT HindIII"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 7481 BP; 1708 A; 1958 C; 2065 G; 1750 T; 0 other;
     gaattcgtcg acccggacgg gacgctcctg cgcctgatac agaacgaatt gcttgcaggc
     atctcatgag tgtgtcttcc cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc
     gcctgctgcg cgacggcgag ctgctcacca cccactcgac cagatccaaa tgagtcttcg
     gacctcgcgg gggccgctta agcggtggtt agggtttgtc tgacgcgggg ggagggggaa
     ggaacgaaac actctcattc ggaggcggct cggggtttgg tcttggtggc cacgggcacg
     cagaagagcg ccgcgatcct cttaagcacc cccccgccct ccgtggaggt gggggtttgg
     tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac
     cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg
     gtcctaggct caatggggac cgtatacgtg gacaggctct ggagcatccg cacgactgcg
     gtgatattac cggagacctt ctgcgggacg agccgggtca cgcggctgac gcggagcgtc
     cgttgggcga caaacaccag gacggggcac aggtacacta tcttgtcacc cggaggcgcg
     agggactgca ggagcttcag ggagtggcgc agctgcttca tccccgtgac ccgttgccgc
     gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct atgatgacac
     aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag tcggggcggc
     gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac accgagcgac
     cctgcagcga cccgcttaac agcgtcaaca gcgtgccgca gatctgctcg acggtactcg
     aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt ttatttcagg
     tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt tgcctttact
     tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt acccgcggcc
     gcaattcccg gggatcgaaa gagcctgcta aagcaaaaaa gaagtcacca tgtcgtttac
     tttgaccaac aagaacgtga ttttcgttgc cggtctggga ggcattggtc tggacaccag
     caaggagctg ctcaagcgcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     caccaacgta acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac ggcagttatc tggaagatca
     ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
     acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
     ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
     atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
     tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
     gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
     gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
     cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg
     gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
     cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta
     cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca
     tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt
     ccaaccgctg tttggtctgc tttctgacaa actcggcctc gactctaggc ggccgcgggg
     atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     tgtgggaggt tttttcggat cctctagagt cgacctgcag gcatgcaagc ttggcgtaat
     catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     cagggttttc ccagtcacga cgttgtaaaa cgacggccag t
//