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ID   PTL1       preliminary; circular DNA; SYN; 5776 BP.
XX
AC   IG9959;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTL1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTL1 from pHC79
RC   pTL3 from pTL1
RC   pTL4 from pTL3
RC   pTL5 from pTL4 & pHC79
RC   pRY2, pRY3, pRY7, pRY13, pRY15 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY1, pRY4, pRY5, pRY6, pRY8 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY9, pRY10, pRY11, pRY12, pRY14 from pTL5 & YIp5 & histone H2A/H2B
RA   Lund T., Grosveld F.G., Flavell R.A.;
RT   "Isolation of transforming DNA by cosmid rescue";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:520-524(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTL1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHC79)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-PstI 5776bp 6523..6524..5775,
FT                   \ lambda cos/tet/ori
FT                   Klenow:Klenow
FT                   -> pTL1 5776bp"
FT   -               1..5776
FT                   /note="pHC79 6523..6524..5774 5776bp
FT                   PstI = CTGCA^G
FT                   EcoRI =    G^AATTC"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda cro-cII region"
FT   misc_feature    0..0
FT                   /note="from bacteriophage Charon 4A,
FT                   from bacteriophage phi 80, right arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A cohesive ends"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A, from
FT                   bacteriophage lambda, left arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda left arm
FT                   #J02459 differs after this position as it contains
FT                   one additional G nucleotide not found in pTL5"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5776 BP; 1310 A; 1617 C; 1455 G; 1394 T; 0 other;
     aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
     aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
     ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
     tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
     cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
     accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg
     atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga
     ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
     attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
     cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
     gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
     acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg
     ggtcctggcc acgggtgcgc atgatctgcc acattacgct cctgtccggc aaagttacct
     ctgccgaagt tgagtatttt tgctgtattt gtcataatga ctcctgttga tagatccagt
     aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
     tattggtgag aatcgcagca acttgtcgcg ccaatcgagc catgtcgtcg tcaacgaccc
     cccattcaag aacagcaagc agcattgaga actttggaat ccagtccctc ttccacctgc
     tgatctgcga cttatcaacg ccgacagctt ccgctgtctt ctcagttcca agcattgcga
     ttttgttaag caacgcactc tcgattcgta gagcctcgtt gcgtttgttt gcacgaacca
     tatgtaagta tttccttaga taacaattga ttgaatgtat gcaaataaat gcatacacca
     taggtgtggt ttaatttgat gccctttttc agggctggaa tgtgtaagag cgggttattt
     atgctgttgt ttttttgtta ctcgggaagg gctttacctc ttccgcataa acgcttccat
     cagcgtttat agttaaaaaa atctttcggc ctgcatgaat ggccttgttg atcgcgattt
     gatatacgcc gagatctaac gggcatcagt aaagtccagc tcctgctggc ggatgacgca
     tgcattatgc tcgcagagat aaaacacgct ggaggggtca tccggcgtcc atttgaggcc
     aaacggcgtc tctttgtcgc caaatttaag atactgctcc tccccgcaat gcgggcaggc
     aacatgaaaa cgcataaaat gcggggattc actggctgca cgctcaatct gacaggtgcc
     tctcactttt ggcgtggagc cacggatgga ctttggccag accgagcctt caatacgctt
     gtcacccagg aacgtcggag agccttcctg ttcaatatca tcatcaaaag cagcaagttc
     atcataaccc gccacatcca ccgacttttc acggtagttt tttgccgctt taccgcccag
     gcaccagaag ccacgcccat tagtgaaacg cttcatggtg agcgtgttaa cccggtgctt
     tttgccatac cacggggcca gcgccagcag cgacggaata tcacgaatag tcggctcaac
     gtgggttttc ataaagttct cggcatcacc atccgtcggc aaccagataa gggtgttgcg
     ctgcttatgc tctataaagt aggcataaac acccagcagc attttggaat aaccgacacg
     ggcagacttc accacattca cctcacggat gtagtcgctg cccatcgcat tcatgatggc
     ccgctgaaag ggcagtgttt cccagcgccc ttcctggtat gcggattctt tcgggagata
     gtaattagca tccgcccatt caacggcggt ctgtggctcc ggcctgaaca gtgagcgaag
     cccggcgcgg acaaaatgcc gcagcctgtt atcctgactg ttcgatatat tcactcagca
     accccggtat cagttcatcc agcgcggctg ctttgttcat ggctttgatg atatcccgtt
     tcaggaaatc aacatgtcgg ttttccagtt ccggaaaacg ccgctgcacc gacaggggga
     gcccgtcgag aatactggca atttcacctg cgatccgcga cagcacgaaa gtacagaatg
     cggttaccac cacttcagcg gagtctctgg cattcttcag ttcctgtgcg tcggcctgcg
     cacgcgtaag tcgatggcgt tcgtactcaa tagttcctgg ctggggatct gcctcgctgg
     cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt ctcaatttca gcatcccttt
     cggcatacca ttttatgacg gcggcagagt cataaagcac ctcattaccc ttgccaccgc
     ctcgcagaac gggcattccc tgttcctgcc agttctgaat ggtacggata ctcgcaccga
     aaatgtcagc cagctgcttt ttgttgactt ccattgttca ttccacggac aaaaacagag
     aaaggaaacg acagaggcca aaaagcctcg ctttcagcac ctgtcgtttc ctttcttttc
     agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac
     cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac ctgtcggatc
     accggaaagg acccgtaaag tgataatgat tatcatctac atatcacaac gtgcgtggag
     gccatcaaac cacgtcaaat aatcaattat gacgcaggta tcgtattaat agatctgcat
     cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
     ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
     acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
     tcgtttcatc ggtatcatta cccccatgaa cagaaattcc cccttacacg gaggcatcaa
     gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta
     acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg
     cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
     atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgctc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctgca
//