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ID   PTL3       preliminary; circular DNA; SYN; 4656 BP.
XX
AC   IG9960;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTL3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTL1 from pHC79
RC   pTL3 from pTL1
RC   pTL4 from pTL3
RC   pTL5 from pTL4 & pHC79
RC   pRY2, pRY3, pRY7, pRY13, pRY15 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY1, pRY4, pRY5, pRY6, pRY8 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY9, pRY10, pRY11, pRY12, pRY14 from pTL5 & YIp5 & histone H2A/H2B
RA   Lund T., Grosveld F.G., Flavell R.A.;
RT   "Isolation of transforming DNA by cosmid rescue";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:520-524(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTL3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHC79)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-PstI 5776bp 6523..6524..5775,
FT                   \ lambda cos/tet/ori
FT                   Klenow:Klenow
FT                   -> pTL1 5776bp
FT                   1. pTL1 BalI-HpaI 4656bp 2567..5775/6523..6524..1447,
FT                   \ lambda cos/tet
FT                   \ BalI 1447 2375/HpaI 2567
FT                   -> pTL3 4656bp"
FT   -               1..3208
FT                   /note="pHC79 2567..5774 3208bp
FT                   PstI = CTGCA^G
FT                   EcoRI =    G^AATTC"
FT   -               3209..4656
FT                   /note="pHC79 6523..6524..1446 1448bp
FT                   BalI = TGG^CCA
FT                   HpaI = GTT^AAC"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda cro-cII region"
FT   misc_feature    0..0
FT                   /note="from bacteriophage Charon 4A,
FT                   from bacteriophage phi 80, right arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A cohesive ends"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A, from
FT                   bacteriophage lambda, left arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda left arm
FT                   #J02459 differs after this position as it contains
FT                   one additional G nucleotide not found in pTL5"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4656 BP; 1046 A; 1328 C; 1199 G; 1083 T; 0 other;
     aattctcatg tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta
     aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
     ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
     ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta
     tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc
     cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg
     accacacccg tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
     acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
     cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
     ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
     ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg
     atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
     cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
     aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
     gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
     tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
     catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga
     ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
     attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
     cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
     gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
     acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg
     ggtcctggaa cccggtgctt tttgccatac cacggggcca gcgccagcag cgacggaata
     tcacgaatag tcggctcaac gtgggttttc ataaagttct cggcatcacc atccgtcggc
     aaccagataa gggtgttgcg ctgcttatgc tctataaagt aggcataaac acccagcagc
     attttggaat aaccgacacg ggcagacttc accacattca cctcacggat gtagtcgctg
     cccatcgcat tcatgatggc ccgctgaaag ggcagtgttt cccagcgccc ttcctggtat
     gcggattctt tcgggagata gtaattagca tccgcccatt caacggcggt ctgtggctcc
     ggcctgaaca gtgagcgaag cccggcgcgg acaaaatgcc gcagcctgtt atcctgactg
     ttcgatatat tcactcagca accccggtat cagttcatcc agcgcggctg ctttgttcat
     ggctttgatg atatcccgtt tcaggaaatc aacatgtcgg ttttccagtt ccggaaaacg
     ccgctgcacc gacaggggga gcccgtcgag aatactggca atttcacctg cgatccgcga
     cagcacgaaa gtacagaatg cggttaccac cacttcagcg gagtctctgg cattcttcag
     ttcctgtgcg tcggcctgcg cacgcgtaag tcgatggcgt tcgtactcaa tagttcctgg
     ctggggatct gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt
     ctcaatttca gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac
     ctcattaccc ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc agttctgaat
     ggtacggata ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt ccattgttca
     ttccacggac aaaaacagag aaaggaaacg acagaggcca aaaagcctcg ctttcagcac
     ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa acattaagtt atgacgaaga
     agaacggaaa cgccttaaac cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc
     gccccgtaac ctgtcggatc accggaaagg acccgtaaag tgataatgat tatcatctac
     atatcacaac gtgcgtggag gccatcaaac cacgtcaaat aatcaattat gacgcaggta
     tcgtattaat agatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct
     gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat
     accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc
     cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaattcc
     cccttacacg gaggcatcaa gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     ctcgctgctc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctgca
//