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ID   PTL4       preliminary; circular DNA; SYN; 4660 BP.
XX
AC   IG9961;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTL4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTL1 from pHC79
RC   pTL3 from pTL1
RC   pTL4 from pTL3
RC   pTL5 from pTL4 & pHC79
RC   pRY2, pRY3, pRY7, pRY13, pRY15 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY1, pRY4, pRY5, pRY6, pRY8 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY9, pRY10, pRY11, pRY12, pRY14 from pTL5 & YIp5 & histone H2A/H2B
RA   Lund T., Grosveld F.G., Flavell R.A.;
RT   "Isolation of transforming DNA by cosmid rescue";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:520-524(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTL4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHC79)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-PstI 5776bp 6523..6524..5775,
FT                   \ lambda cos/tet/ori
FT                   Klenow:Klenow
FT                   -> pTL1 5776bp
FT                   1. pTL1 BalI-HpaI 4656bp 2567..5775/6523..6524..1447,
FT                   \ lambda cos/tet
FT                   \ BalI 1447 2375/HpaI 2567
FT                   -> pTL3 4656bp
FT                   1. pTL3 BglII 4200bp 3830..3830, 3' lambda cos
FT                   Klenow
FT                   -> pTL4 4660bp"
FT   -               1..1267
FT                   /note="pHC79 2567..3833 1267bp
FT                   BglII = A^GATC T
FT                   BglII =      A^GATCT"
FT   -               1268..3212
FT                   /note="pHC79 3830..5774 1945bp
FT                   PstI = CTGCA^G
FT                   EcoRI =    G^AATTC"
FT   -               3213..4660
FT                   /note="pHC79 6523..6524..1446 1448bp
FT                   BalI = TGG^CCA
FT                   HpaI = GTT^AAC"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda cro-cII region"
FT   misc_feature    0..0
FT                   /note="from bacteriophage Charon 4A,
FT                   from bacteriophage phi 80, right arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A cohesive ends"
FT   misc_feature    0..0
FT                   /note="bacteriophage Charon 4A, from
FT                   bacteriophage lambda, left arm region"
FT   misc_feature    0..0
FT                   /note="bacteriophage lambda left arm
FT                   #J02459 differs after this position as it contains
FT                   one additional G nucleotide not found in pTL5"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4660 BP; 1047 A; 1329 C; 1200 G; 1084 T; 0 other;
     aacccggtgc tttttgccat accacggggc cagcgccagc agcgacggaa tatcacgaat
     agtcggctca acgtgggttt tcataaagtt ctcggcatca ccatccgtcg gcaaccagat
     aagggtgttg cgctgcttat gctctataaa gtaggcataa acacccagca gcattttgga
     ataaccgaca cgggcagact tcaccacatt cacctcacgg atgtagtcgc tgcccatcgc
     attcatgatg gcccgctgaa agggcagtgt ttcccagcgc ccttcctggt atgcggattc
     tttcgggaga tagtaattag catccgccca ttcaacggcg gtctgtggct ccggcctgaa
     cagtgagcga agcccggcgc ggacaaaatg ccgcagcctg ttatcctgac tgttcgatat
     attcactcag caaccccggt atcagttcat ccagcgcggc tgctttgttc atggctttga
     tgatatcccg tttcaggaaa tcaacatgtc ggttttccag ttccggaaaa cgccgctgca
     ccgacagggg gagcccgtcg agaatactgg caatttcacc tgcgatccgc gacagcacga
     aagtacagaa tgcggttacc accacttcag cggagtctct ggcattcttc agttcctgtg
     cgtcggcctg cgcacgcgta agtcgatggc gttcgtactc aatagttcct ggctggggat
     ctgcctcgct ggcctgccgc agttcttcaa cctcccggcg cagcttttcg ttctcaattt
     cagcatccct ttcggcatac cattttatga cggcggcaga gtcataaagc acctcattac
     ccttgccacc gcctcgcaga acgggcattc cctgttcctg ccagttctga atggtacgga
     tactcgcacc gaaaatgtca gccagctgct ttttgttgac ttccattgtt cattccacgg
     acaaaaacag agaaaggaaa cgacagaggc caaaaagcct cgctttcagc acctgtcgtt
     tcctttcttt tcagagggta ttttaaataa aaacattaag ttatgacgaa gaagaacgga
     aacgccttaa accggaaaat tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta
     acctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct acatatcaca
     acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg tatcgtatta
     atagatcgat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
     ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
     tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct
     tacacggagg catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     ctgctctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgctg caaattctca tgtttgacag cttatcatcg
     ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
     tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
     taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
     tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
     ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
     ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg
     ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
     gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     cggctcttac cagcctaact tcgatcactg gaccgctgat cgtcacggcg atttatgccg
     cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga atggaagccg
     gcggcacctc gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag
     aactgtgaat gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag
     cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg
//