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ID   PTL5       preliminary; circular DNA; SYN; 5793 BP.
XX
AC   U07340;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pTL5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5793
RC   pTL5 from pTL4 & pHC79
RA   Slightom J.L., Siemieniak D.R., Koop B.F., Hood L.;
RT   "The complete nucleotide sequence of cosmid vector pTL5: location and
RT   origin of its genetic components";
RL   Gene 147:77-79(1994).
XX
RN   [2]
RP   1461-2113; 3437-3854
RC   lambda from E.coli
RA   Daniels D.L., Schroeder J.L., Szybalski W., Sanger F.,
RA   Blattner F.R.;
RT   "Appendix I: A Molecular Map of Coliphage Lambda";
RL   Lambda II 0:519-674(1983).
RL   Hendrix R.W., ed.,
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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RN   [3]
RP   1-5793
RC   pTL1 from pHC79
RC   pTL3 from pTL1
RC   pTL4 from pTL3
RC   pTL5 from pTL4 & pHC79
RC   pRY2, pRY3, pRY7, pRY13, pRY15 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY1, pRY4, pRY5, pRY6, pRY8 from pTL5 & YIp5 & histone H2A/H2B
RC   pRY9, pRY10, pRY11, pRY12, pRY14 from pTL5 & YIp5 & histone H2A/H2B
RA   Lund T., Grosveld F.G., Flavell R.A.;
RT   "Isolation of transforming DNA by cosmid rescue";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:520-524(1982).
XX
RN   [4]
RP   1-5793
RC   pTL5
RA   Slightom J.L.;
RT   "Isolation of transforming DNA by cosmid rescue";
RL   Submitted (02-MAR-1994) by:
RL   Slightom J.L., Molecular Biology,
RL   Upjohn Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA.
XX
RN   [5]
RC   pYIp5-TRT-1 from YIp5 & yeast histone H2A/H2B
RA   Hereford L.;
RT   ;
RL   Unpublished (1982).
XX
CC   NCBI gi: 460969
CC   NM (pTL5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pHC79 EcoRI-PstI 5776bp 6523..6524..5775,
FT                   \ lambda cos/tet/ori
FT                   Klenow:Klenow
FT                   -> pTL1 5776bp
FT                   1. pTL1 BalI-HpaI 4656bp 2567..5775/6523..6524..1447,
FT                   \ lambda cos/tet
FT                   \ BalI 1447 2375/HpaI 2567
FT                   -> pTL3 4656bp
FT                   1. pTL3 BglII 4656bp 3830..3830, 3' lambda cos
FT                   Klenow
FT                   -> pTL4 4656bp
FT                   1. pTL4 HindIII-BstEII 2642bp 30..-..3181,
FT                   \ lambda cos/no tet
FT                   2. pHC79 HindIII-BstEII 3151bp 30..3181,
FT                   \ lambda cos/tet
FT                   -> pTL5 5793bp"
FT   misc_feature    1461..2113
FT                   /note="bacteriophage lambda cro-cII region"
FT   misc_feature    2114..3448
FT                   /note="from bacteriophage Charon 4A,
FT                   from bacteriophage phi 80, right arm region"
FT   misc_feature    3437..3448
FT                   /note="bacteriophage Charon 4A cohesive ends"
FT   misc_feature    3449..3854
FT                   /note="bacteriophage Charon 4A, from
FT                   bacteriophage lambda, left arm region"
FT   misc_feature    3573..3573
FT                   /note="bacteriophage lambda left arm
FT                   #J02459 differs after this position as it contains
FT                   one additional G nucleotide not found in pTL5"
FT   rep_origin      3855..5793
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5793 BP; 1351 A; 1529 C; 1480 G; 1433 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatctgccac attacgctcc tgtccggcaa agttacctct
     gccgaagttg agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa
     tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc aacgaccccc
     cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt ccacctgctg
     atctgcgact tatcaacgcc cacagcttcc gctgtcttct cagttccaag cattgcgatt
     ttgttaagca acgcactctc gattcgtaga gcctcgttgc gtttgtttgc acgaaccata
     tgtaagtatt tccttagata acaattgatt gaatgtatgc aaataaatgc atacaccata
     ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg gggttattta
     tgctgttgtt tttttgttac tcgggaaggg ctttacctct tccgcataaa cgcttccatc
     agcgtttata gttaaaaaaa tctttcggcc tgcatgaatg gccttgttga tcgcgctttg
     atatacgccg agatcttggc agtcacagca tgcgcatatc catgcttcga ccatgcgctc
     acaaagtagg tgaatgcgca atgtagtacc cacatcgtca tcgctttcca ctgctctcgc
     gaataaagat ggaaaatcaa tctcatggta atagtccatg aaaatccttg tattcataaa
     tcctccaggt agctatatgc aaattgaaac aaaagagatg gtgatctttc taagagatga
     tggaatctcc cttcagtatc ccgatggtca atgcgctgga tatgggatag atgggaatat
     gctgattttt atgggacaga gttgcgaact gttcccaact aaaatcattt tgcacgatca
     gcgcactacg aactttaccc acaaatagtc aggtaatgaa tcctgatata aagacaggtt
     gataaatcag tcttctacgc gcatcgcacg cgcacaccgt agaaagtctt tcagttgtga
     gcctgggcaa accgttaact ttcggcggct ttgctgtgcg acaggctcac gtctaaaagg
     aaataaatca tgggtcataa aattatcacg ttgtccggcg cggcgacgga tgttctgtat
     gcgctgtttt tccgtggcgc gttgctgtct ggtgatctgc cttctaaatc tggcacagcc
     gaattgcgcg agcttggttt tgctgaaacc agacacacag caactgaata ccagaaagaa
     aatcacttta cctttctgac atcagaaggg cagaaatttg ccgttgaaca cctggtcaat
     acgcgttttg gtgagcagca atattgcgct tcgatgacgc ttggcgttga gattgatacc
     tctgctgcac aaaaggcaat cgacgagctg gaccagcgca ttcgtgacac cgtctccttc
     gaacttattc gcaatggagt gtcattcatc aaggacgccg ctatcgcaaa tggtgctatc
     cacgcagcgg caatcgaaac acctcagccg gtgaccaata tctacaacat cagccttggt
     atccagcgtg atgagccagc gcagaacaag gtaaccgtca gtgccgataa gttcaaagtt
     aaacctggtg ttgataccaa cattgaaacg ttgatcgaaa acgcgctgaa aaacgctgct
     gaatgtgcgg cgctggatgt cacaaagcaa atggcagcag acaagaaagc gatggatgaa
     ctggcttcct atgtccgcac ggccatcatg atggaatgtt tccccggtgg tgttatctgg
     cagcagtgcc gtcgatagta tgcaattgat aattattatc atttgcgggt cctttccggc
     gatccgcctt gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg
     gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct
     gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc
     tctgtttttg tccgtggaat gaacaatgga agtcaacaaa aagcagctgg ctgacatttt
     cggtgcgagt atccgtacca ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg
     cggtggcaag ggtaatgagg tgctttatga ctctgccgcc gtcataaaat ggtatgccga
     aagggatgct gaaattgaga acgaaaagct gcgccgggag gttgaagaac tgcggcaggc
     cagcgaggca gatcgatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
     tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
     caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
     gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     agttaatagt ttgcgcaacg ttgttgccat taa
//