Return to this vector's summary.
ID PTL5 preliminary; circular DNA; SYN; 5793 BP.
XX
AC U07340;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pTL5 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-5793
RC pTL5 from pTL4 & pHC79
RA Slightom J.L., Siemieniak D.R., Koop B.F., Hood L.;
RT "The complete nucleotide sequence of cosmid vector pTL5: location and
RT origin of its genetic components";
RL Gene 147:77-79(1994).
XX
RN [2]
RP 1461-2113; 3437-3854
RC lambda from E.coli
RA Daniels D.L., Schroeder J.L., Szybalski W., Sanger F.,
RA Blattner F.R.;
RT "Appendix I: A Molecular Map of Coliphage Lambda";
RL Lambda II 0:519-674(1983).
RL Hendrix R.W., ed.,
RL Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
XX
RN [3]
RP 1-5793
RC pTL1 from pHC79
RC pTL3 from pTL1
RC pTL4 from pTL3
RC pTL5 from pTL4 & pHC79
RC pRY2, pRY3, pRY7, pRY13, pRY15 from pTL5 & YIp5 & histone H2A/H2B
RC pRY1, pRY4, pRY5, pRY6, pRY8 from pTL5 & YIp5 & histone H2A/H2B
RC pRY9, pRY10, pRY11, pRY12, pRY14 from pTL5 & YIp5 & histone H2A/H2B
RA Lund T., Grosveld F.G., Flavell R.A.;
RT "Isolation of transforming DNA by cosmid rescue";
RL Proc. Natl. Acad. Sci. U.S.A. 79:520-524(1982).
XX
RN [4]
RP 1-5793
RC pTL5
RA Slightom J.L.;
RT "Isolation of transforming DNA by cosmid rescue";
RL Submitted (02-MAR-1994) by:
RL Slightom J.L., Molecular Biology,
RL Upjohn Company, 301 Henrietta Street, Kalamazoo, MI 49007, USA.
XX
RN [5]
RC pYIp5-TRT-1 from YIp5 & yeast histone H2A/H2B
RA Hereford L.;
RT ;
RL Unpublished (1982).
XX
CC NCBI gi: 460969
CC NM (pTL5)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pBR322)(lambda)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pHC79 EcoRI-PstI 5776bp 6523..6524..5775,
FT \ lambda cos/tet/ori
FT Klenow:Klenow
FT -> pTL1 5776bp
FT 1. pTL1 BalI-HpaI 4656bp 2567..5775/6523..6524..1447,
FT \ lambda cos/tet
FT \ BalI 1447 2375/HpaI 2567
FT -> pTL3 4656bp
FT 1. pTL3 BglII 4656bp 3830..3830, 3' lambda cos
FT Klenow
FT -> pTL4 4656bp
FT 1. pTL4 HindIII-BstEII 2642bp 30..-..3181,
FT \ lambda cos/no tet
FT 2. pHC79 HindIII-BstEII 3151bp 30..3181,
FT \ lambda cos/tet
FT -> pTL5 5793bp"
FT misc_feature 1461..2113
FT /note="bacteriophage lambda cro-cII region"
FT misc_feature 2114..3448
FT /note="from bacteriophage Charon 4A,
FT from bacteriophage phi 80, right arm region"
FT misc_feature 3437..3448
FT /note="bacteriophage Charon 4A cohesive ends"
FT misc_feature 3449..3854
FT /note="bacteriophage Charon 4A, from
FT bacteriophage lambda, left arm region"
FT misc_feature 3573..3573
FT /note="bacteriophage lambda left arm
FT #J02459 differs after this position as it contains
FT one additional G nucleotide not found in pTL5"
FT rep_origin 3855..5793
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 5793 BP; 1351 A; 1529 C; 1480 G; 1433 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatctgccac attacgctcc tgtccggcaa agttacctct
gccgaagttg agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa
tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc aacgaccccc
cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt ccacctgctg
atctgcgact tatcaacgcc cacagcttcc gctgtcttct cagttccaag cattgcgatt
ttgttaagca acgcactctc gattcgtaga gcctcgttgc gtttgtttgc acgaaccata
tgtaagtatt tccttagata acaattgatt gaatgtatgc aaataaatgc atacaccata
ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg gggttattta
tgctgttgtt tttttgttac tcgggaaggg ctttacctct tccgcataaa cgcttccatc
agcgtttata gttaaaaaaa tctttcggcc tgcatgaatg gccttgttga tcgcgctttg
atatacgccg agatcttggc agtcacagca tgcgcatatc catgcttcga ccatgcgctc
acaaagtagg tgaatgcgca atgtagtacc cacatcgtca tcgctttcca ctgctctcgc
gaataaagat ggaaaatcaa tctcatggta atagtccatg aaaatccttg tattcataaa
tcctccaggt agctatatgc aaattgaaac aaaagagatg gtgatctttc taagagatga
tggaatctcc cttcagtatc ccgatggtca atgcgctgga tatgggatag atgggaatat
gctgattttt atgggacaga gttgcgaact gttcccaact aaaatcattt tgcacgatca
gcgcactacg aactttaccc acaaatagtc aggtaatgaa tcctgatata aagacaggtt
gataaatcag tcttctacgc gcatcgcacg cgcacaccgt agaaagtctt tcagttgtga
gcctgggcaa accgttaact ttcggcggct ttgctgtgcg acaggctcac gtctaaaagg
aaataaatca tgggtcataa aattatcacg ttgtccggcg cggcgacgga tgttctgtat
gcgctgtttt tccgtggcgc gttgctgtct ggtgatctgc cttctaaatc tggcacagcc
gaattgcgcg agcttggttt tgctgaaacc agacacacag caactgaata ccagaaagaa
aatcacttta cctttctgac atcagaaggg cagaaatttg ccgttgaaca cctggtcaat
acgcgttttg gtgagcagca atattgcgct tcgatgacgc ttggcgttga gattgatacc
tctgctgcac aaaaggcaat cgacgagctg gaccagcgca ttcgtgacac cgtctccttc
gaacttattc gcaatggagt gtcattcatc aaggacgccg ctatcgcaaa tggtgctatc
cacgcagcgg caatcgaaac acctcagccg gtgaccaata tctacaacat cagccttggt
atccagcgtg atgagccagc gcagaacaag gtaaccgtca gtgccgataa gttcaaagtt
aaacctggtg ttgataccaa cattgaaacg ttgatcgaaa acgcgctgaa aaacgctgct
gaatgtgcgg cgctggatgt cacaaagcaa atggcagcag acaagaaagc gatggatgaa
ctggcttcct atgtccgcac ggccatcatg atggaatgtt tccccggtgg tgttatctgg
cagcagtgcc gtcgatagta tgcaattgat aattattatc atttgcgggt cctttccggc
gatccgcctt gttacggggc ggcgacctcg cgggttttcg ctatttatga aaattttccg
gtttaaggcg tttccgttct tcttcgtcat aacttaatgt ttttatttaa aataccctct
gaaaagaaag gaaacgacag gtgctgaaag cgagcttttt ggcctctgtc gtttcctttc
tctgtttttg tccgtggaat gaacaatgga agtcaacaaa aagcagctgg ctgacatttt
cggtgcgagt atccgtacca ttcagaactg gcaggaacag ggaatgcccg ttctgcgagg
cggtggcaag ggtaatgagg tgctttatga ctctgccgcc gtcataaaat ggtatgccga
aagggatgct gaaattgaga acgaaaagct gcgccgggag gttgaagaac tgcggcaggc
cagcgaggca gatcgatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
agttaatagt ttgcgcaacg ttgttgccat taa
//