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ID   PTL61T     preliminary; circular DNA; SYN; 8565 BP.
XX
AC   M29896;
XX
DT   15-SEP-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTL61T - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8565
RC   pTL38 from pTL25 & pKK161-8, rrnB T1T2
RC   lambda TL39 from pTL38 & lambda L47.1 & lambda TL25
RC   pTL40 from pTL38
RC   pTL61T from pTL40 & oligo & pRS415 & pAR3435, R1.1 RNaseIII site
RC   lambda TL61 from lambda TL39 & pTL61T
RA   Linn T.S., St Pierre R.;
RT   "Improved vector system for constructing transcriptional fusions
RT   that ensures independent translation of lacZ";
RL   J. Bacteriol. 172:1077-1084(1990).
XX
RN   [2]
RP   3274-3279
RC   pTL61T
RA   Linn T.S.;
RT   ;
RL   Submitted (15-NOV-1989) to GenBank on computer-readable media by:
RL   Linn T.S., .
XX
RN   [3]
RC   pGR5-2 from pBR325 & lambda JDW36
RC   pTL25 from pFR109 & pGR5-2
RC   lambda TL25 from lambda NM616 & lambda RS205 & pTL25
RC   lambda GR8 from lambda JDW36 & rplKp gene
RC   lambda TL108 from lambda TL25 & rplKp gene
RC   lambda TL116 from lambda TL25 & rplKp gene
RA   Linn T., Ralling G.;
RT   "A versatile multiple and single copy vector system for the in vitro
RT   construction of transcriptional fusions to lacZ";
RL   Plasmid 14:134-142(1985).
XX
RN   [4]
RC   lambda KV4 from lambda
RA   Ralling G., Linn T.;
RT   ;
RL   Unpublished (1988).
XX
RN   [5]
RC   lambda RS205 from Charon 16 & lambda W205
RC   from lambda RS205 & pBR322
RC   pBT401 from pACYC177 & Tn10
RC   pBT402 from pACYC177 & Tn10
RA   Bertrand K.P., Postle K., Wray L.V., Reznikoff W.S.;
RT   "Construction of a single-copy promoter vector and its use in
RT   analysis of regulation of the transposon Tn10 tetracycline
RT   resistance determinant";
RL   J. Bacteriol. 158:910-919(1984).
XX
RN   [6]
RC   ColE1:Tn10 from ColE1 & Tn10, tet gene
RC   pRT29 from pVH51 & ColE1:Tn10, tet gene
RA   Jorgensen R.A., Reznikoff W.S.;
RT   "Organziation of structural and regulatory genes that mediate
RT   tetracycline resistance in transposon Tn10";
RL   J. Bacteriol. 138:705-714(1979).
XX
RN   [7]
RC   pRT210 from pRZ112 & ColE1:Tn10, tet gene
RA   Wray L.V., Jorgensen R.A., Reznikoff W.S.;
RT   "Identification of the tetracycline resistance promoter and repressor
RT   in transposon Tn10";
RL   J. Bacteriol. 147:297-304(1981).
XX
RN   [8]
RC   lambda JDW19 from lambda imm21
RC   lambda JDW36 from lambda JDW19
RA   Windass J.D., Brammar W.J.;
RT   "Aberrant immunity behavior of hybrid lambda imm21 phages containing
RT   the DNA of ColE1-type plasmids";
RL   Mol. Gen. Genet. 172:329-337(1979).
XX
RN   [9]
RC   lambda NM616 from lambda
RA   Wilson G.G., Murray N.E.;
RT   "Molecular cloning of the DNA ligase gene from bacteriophage T4";
RL   J. Mol. Biol. 132:471-491(1979).
XX
CC   NCBI gi: 209190
CC   NM (pTL61T)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)(fusion)
CC   SE (color blue/white)
CC   PA (pBR322)(pRS551)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda imm21
FT                   -> lambda JDW19
FT                   1. lambda JDW19
FT                   -> lambda JDW36
FT                   1. pBR325 remove EcoRI-HindIII 1246bp 4778..5994..30,
FT                   \ 4750bp
FT                   2. lambda JDW36 EcoRI-HindIII 5200bp, trpB/lacZ
FT                   -> pGR5-2 6450bp
FT                   1. pFR109 remove AatII-SmaI 150bp 3569..3719,
FT                   \ lacZ/9748bp
FT                   2. pGR5-2 RsaI-AatII 721bp, deltaW205 of trpB/lacZ
FT                   -> pTL25 10469bp [no RsaI, no SmaI]
FT                   1. pTL25 BamHI 10469bp, 3' trpA gene
FT                   BAL31 nuclease, to remove trp terminator
FT                   SmaI linker 6bp cccggg
FT                   AvaI 10000bp, pBR322
FT                   2. pKK161-8 AvaI 5260bp 5227..5227, rrnB T1T2
FT                   -> pTL38 15000bp
FT                   1. lambda cI857 Sam7
FT                   2. E.coli trp/lac W205 delta trp11/14-1c
FT                   -> lambda W205
FT                   1. Charon 16 EcoRI, 3' lacZ gene
FT                   mutation AA 1006-1007
FT                   -> lambda X616
FT                   1. lambda X616
FT                   -> lambda X616J
FT                   1. lambda X616J
FT                   2. lambda W205
FT                   -> lambda W205JR
FT                   1. lambda W2001 remove SalI-SalI 499bp
FT                   M. luteus DNA polymerase
FT                   -> lambda W2001SP [no SalI]
FT                   1. pVH51 remove ?-KpnI 380bp ?..1982, 3470bp
FT                   2. pJG10 EcoRI-HindIII 820bp, E. coli thr promoter
FT                   3. lambda W205JR HindIII-KpnI 6000bp, trpA/lacZ
FT                   -> pRS205 10300bp [unique EcoRI, SalI in trpA/lacZ]
FT                   1. lambda X616 SstI-SstI
FT                   1. pRS205 SstI-EcoRI
FT                   2. lambda W2001SP EcoRI, right arm
FT                   -> lambda RS205 cIam
FT                   1. lambda RS205 cIam XhoI, remove right arm
FT                   2. lambda gt.lambdaC XhoI, right arm
FT                   -> lambda RS205
FT                   1. lambda
FT                   -> lambda NM616
FT                   1. lambda RS205 left end-SacI, lacZ
FT                   2. pTL25 SacI-EcoRI, lacZ
FT                   3. lambda NM616 EcoRI-right end, right arm
FT                   -> lambda TL25
FT                   1. pTL38 EcoRI-BalI, MCS to rrnB term
FT                   2. lambda L47.1 end-SmaI, left arm
FT                   3. lambda TL25 EcoRI-end, right arm
FT                   -> lambda TL39
FT                   1. pTL38 remove DraI-NaeI, 5' lacY to pBR322 1283
FT                   -> pTL40
FT                   1. pBR322 4361bp
FT                   2. R1.1 site
FT                   -> pAR3433 4400bp
FT                   1. pAR3433 4400bp
FT                   -> pAR3435 4400bp
FT                   1. pTL40 remove BamHI-AvrII, MCS
FT                   2. pAR3435 BamHI-BglII 93bp, R1.1 RNaseIII site
FT                   -> plasmid
FT                   1. plasmid BamHI
FT                   2. oligo BamHI-BamHI, 3 frame terminator codons
FT                   -> plasmid2
FT                   1. plasmid2 remove ScaI-EcoRI, amp to MCS/4369bp
FT                   2. pRS415 ScaI-EcoRI 4196bp 5001..6021..3176,
FT                   \ rrnB T1 terminator
FT                   -> pTL61T 8565bp"
FT   misc_recomb     join(8565..8565,1..1)
FT                   /note="pRS551 DNA end/synthetic DNA start"
FT   misc_binding    1..92
FT                   /note="MCS"
FT   misc_binding    116..192
FT                   /note="SIT E. coli RNaseIII processing site"
FT   misc_recomb     238..239
FT                   /note="synthetic DNA end/E. coli DNA start"
FT   CDS             256..3330
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   mutation        3274..3279
FT                   /note="alters the EcoRI site in E. coli lacZ gene"
FT   misc_recomb     3392..3393
FT                   /note="E. coli DNA end/pBR322 DNA start"
FT   rRNA            3971..4470
FT                   /note="RNA E. coli 5S rRNA"
FT   rRNA            4090..4588
FT                   /note="RNA E. coli 5S rRNA"
FT   misc_recomb     4886..4887
FT                   /note="E. coli DNA end/pBR322 DNA start"
FT   mutation        7071..7073
FT                   /note="TGC in pBR322; TAC in pTL61T"
FT   mutation        7824..7826
FT                   /note="A in pBR322; ATT in pTL61T"
FT   misc_recomb     7826..7827
FT                   /note="pBR322 DNA end/pRS551 DNA start"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 8565 BP; 2028 A; 2266 C; 2333 G; 1938 T; 0 other;
     gaattctcat gtttgacagc ttatcatcgg agctctcgag tcagcccggg cgaatcgata
     agcttgggct gcaggtcgac tctagaggat cctaactaac tagcgatccc gactcactat
     agagggacaa actcaaggtc attcgcaaga gtggccttta tgattgacct tcttccggtt
     aatacgaccg ggatcgagat cctaggtagg taggggcgcg gcattttaac tttctttatc
     acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt
     gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg
     gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt
     tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt
     cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta
     caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc
     aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc
     gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc
     agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg
     ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat
     ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta
     cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc
     ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac
     gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa
     ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag
     gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt
     aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag
     gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac
     catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat
     attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg
     gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc
     atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg
     atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg
     gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct
     gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc
     ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag
     gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg
     ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat
     acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat
     ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa
     accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg
     atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca
     caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc
     tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc
     agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc
     cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag
     cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa
     caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc
     gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc
     cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg
     ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa
     acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc
     gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg
     gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt
     tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa
     aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac
     ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat
     ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt
     ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac
     cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta
     tttcgcgtaa ggaaatccat tatgtactat ttggcatggc ggccgacgcg ctgggctacg
     tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg ctgttttggc
     ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata
     aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca
     gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac
     tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt
     tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca
     aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt
     cgtcatatct acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag
     gaaagcagct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa
     cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct
     gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc
     catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
     ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt
     tctacaaact cttcctgtcg tcatatctac aagccatccc cccacagata cggtaaacta
     gcctcgtttt tgcatcagga aagcagtcgg gcagcgttgg gtcctggcca cgggtgcgca
     tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc
     agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga
     cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga
     agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct
     gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct
     ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat
     gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc
     ccatgaacag aaattccccc ttacacggag gcatcaagtg accaaacagg aaaaaaccgc
     ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtcttca agaattaatt cccaattcca ggcatcaaat aaaacgaaag
     gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg
     cgggcaggac gcccgccata aactgccagg aattaattcc aggcatcaaa taaaacgaaa
     ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     gcgggcagga cgcccgccat aaactgccag gaattaattc caggcatcaa ataaaacgaa
     aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     ggcgggcagg acgcccgcca taaactgcca ggaattaatt ccaggcatca aataaaacga
     aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     tggcgggcag gacgcccgcc ataaactgcc aggaattggg gatcg
//