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ID   PTRC99A    preliminary; circular DNA; SYN; 4176 BP.
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AC   M22744; U13872; A13038;
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DT   15-JUN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pTrc99A - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4176
RC   pFXIII from FXIIIa fragment
RC   pTrc89-1 from pKK233-2 & linker
RC   pTrc96A, pTrc96B, pTrc96C from pTrc89-1 & oligos
RC   pTrc97A from pTrc96A & pUC18
RC   pTrc97B from pTrc96B & pUC18
RC   pTrc97C from pTrc96C & pUC18
RC   pTrc90-3 from pTrc89-1 & placIq
RC   pTrc98A, pTrc98B, pTrc98C from pTrc90-3 & oligos
RC   pTrc99A from pTrc98A & pUC18
RC   pTrc99B from pTrc98B & pUC18
RC   pTrc99C from pTrc98C & pUC18
RC   pMa5-8-PP4 from lambda gt11 & pMa5-8
RC   pMa5-8-PP4-NcoI from pMa5-8-PP4
RC   pTrc99A-PP4 from pTrc99A & pMa5-8-PP4-NcoI
RC   pTrc97 from E. coli rrnB T1T2
RA   Amann E., Ochs B., Abel K.J.;
RT   "Tightly regulated tac promoter vectors useful for the expression of
RT   unfused and fused proteins in Escherichia coli";
RL   Gene 69:301-315(1988).
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RN   [2]
RC   pMa5-8 from pcIwt & pBR325 & pEMBL8+ & pPLcAT14-betagal
RA   Fritz H.J.;
RT   ;
RL   Unpublished (1988).
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RN   [3]
RC   placIq from lacIq gene
RA   Wang J.;
RT   ;
RL   Unpublished (1988).
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CC   NM (pTrc99A)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR (pTrc97)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2
FT                   2. linker
FT                   -> pTrc89-1
FT                   1. pTrc89-1 BglII
FT                   fill in
FT                   2. placIq EcoRI-EcoRI, lacIq gene
FT                   fill in:fill in
FT                   -> pTrc90-3 4100bp
FT                   1. pTrc90-3 NcoI-HindIII 4100bp
FT                   2. oligo catggaattcga
FT                   -> pTrc98A 4100bp
FT                   1. pTrc98A EcoRI-HindIII 4100bp
FT                   2. pUC18 HindIII-EcoRI 51bp 400..451, MCS [55bp]
FT                   -> pTrc99A 4176bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 4176 BP; 1009 A; 1059 C; 1118 G; 990 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagaccatgg aattcgagct cggtacccgg ggatcctcta
     gagtcgacct gcaggcatgc aagcttggct gttttggcgg atgagagaag attttcagcc
     tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
     atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     cgatgcctac agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
     ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc
     tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     caacgcaatt aatgtgagtt agcgcgaatt gatctg
//