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ID   PTRCHISA   preliminary; circular DNA; SYN; 4414 BP.
XX
AC   IG1131;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTRCHISA - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
CC   NM (pTRCHISA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pSE420)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSE420 remove, MCS/4613bp-
FT                   2. oligo, ATG/his codon repeats/enterokinase site
FT                   -> pTRCHISA 4414bp"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacO gene"
FT   promoter        0..0
FT                   /note="PRO E. coli antitermination"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   misc_feature    0..0
FT                   /note="E. coli mini-cistron"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-AvaI-XhoI-BglII-PstI-KpnI-EcoRI-BstBI-HindIII"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1123..1123
FT                   /note="SIT PvuI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2824..2824
FT                   /note="SIT SphI"
FT   promoter        0..0
FT                   /note="PRO E. coli lacIq gene"
FT   misc_binding    3474..3474
FT                   /note="SIT SphI"
FT   misc_binding    3715..3715
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 4414 BP; 1087 A; 1094 C; 1178 G; 1055 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
     caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
     aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
     ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
     ggatctgtac gacgatgacg ataaggatcg atggggatcc gagctcgaga tctgcagctg
     gtaccatatg ggaattcgaa gcttggctgt tttggcggat gagagaagat tttcagcctg
     atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
     agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
     ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
     ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
     gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
     ggatggcctt tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat
     atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt
     cgcgcgcgaa ggcgaagcgg catgcattta cgttgacacc atcgaatggc gcaaaacctt
     tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
     agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
     ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
     ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
     gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
     taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
     cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
     cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
     tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
     tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     cccgccgtca accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     acgcaattaa tgtgagttag cgcgaattga tctg
//