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ID   PTRCHISB   preliminary; circular DNA; SYN; 4404 BP.
XX
AC   IG1132;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTRCHISB - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
CC   NM (pTRCHISB)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pSE420)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSE420 remove, MCS/4613bp-
FT                   2. oligo, ATG/his codon repeats/enterokinase site
FT                   -> pTRCHISB 4404bp"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacO gene"
FT   promoter        0..0
FT                   /note="PRO E. coli antitermination"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   misc_feature    0..0
FT                   /note="E. coli mini-cistron"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-AvaI-XhoI-BglII-PstI-KpnI-EcoRI-BstBI-HindIII"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1123..1123
FT                   /note="SIT PvuI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2824..2824
FT                   /note="SIT SphI"
FT   promoter        0..0
FT                   /note="PRO E. coli lacIq gene"
FT   misc_binding    3474..3474
FT                   /note="SIT SphI"
FT   misc_binding    3715..3715
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 4404 BP; 1085 A; 1093 C; 1173 G; 1053 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
     caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
     aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
     ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
     ggatctgtac gacgatgacg ataaggatcc gagctcgaga tctgcagctg gtaccatatg
     ggaattcgaa gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta
     aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg
     tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg
     ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc
     tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa
     ggcgaagcgg catgcattta cgttgacacc atcgaatggc gcaaaacctt tcgcggtatg
     gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta
     tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag
     gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat
     tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt
     gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc
     gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc
     tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat
     ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta
     tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt
     acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg
     ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact
     cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt
     caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac
     gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg
     gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtca
     accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     tgtgagttag cgcgaattga tctg
//