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ID   PTRCHISC   preliminary; circular DNA; SYN; 4412 BP.
XX
AC   IG1133;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTRCHISC - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pRSETA from pET-3 & pBluescript series
RC   pRSETB from pET-3 & pBluescript series
RC   pRSETC from pET-3 & pBluescript series
RC   pSE280 from pKK233-2
RC   pSE380 from pTrc99A
RC   pSE420 from pSE380
RC   pTRCHISA from pSE420
RC   pTRCHISB from pSE420
RC   pTRCHISC from pSE420
RA   Abdel-Malek H.;
RT   ;
RL   The Digest 4:1-3(1991).
XX
CC   NM (pTRCHISC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pSE420)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSE420 remove, MCS/4613bp-
FT                   2. oligo, ATG/his codon repeats/enterokinase site
FT                   -> pTRCHISC 4412bp"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   promoter        0..0
FT                   /note="PRO E. coli lacO gene"
FT   promoter        0..0
FT                   /note="PRO E. coli antitermination"
FT   RBS             0..0
FT                   /note="RBS E. coli g10 gene"
FT   misc_feature    0..0
FT                   /note="E. coli mini-cistron"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   repeat_unit     0..0
FT                   /note="RPT histidine (6)"
FT   misc_binding    0..0
FT                   /note="SIT NheI"
FT   misc_binding    0..0
FT                   /note="SIT enterokinase (EK)"
FT   misc_binding    0..0
FT                   /note="MCS
FT                   BamHI-AvaI-XhoI-BglII-PstI-KpnI-EcoRI-BstBI-HindIII"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1123..1123
FT                   /note="SIT PvuI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2824..2824
FT                   /note="SIT SphI"
FT   promoter        0..0
FT                   /note="PRO E. coli lacIq repressor gene"
FT   misc_binding    3474..3474
FT                   /note="SIT SphI"
FT   misc_binding    3715..3715
FT                   /note="SIT EcoRV"
XX
SQ   Sequence 4412 BP; 1088 A; 1094 C; 1175 G; 1055 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
     taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa
     caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta
     aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg
     ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg
     ggatctgtac gacgatgacg ataaggatcg atggatccga cctcgagatc tgcagatggt
     accatatggg aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
     acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
     cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
     tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
     gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
     atggcctttt tgcgtttcta caaactcttt ttgtttattt ttctaaatac attcaaatat
     gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
     cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggcgc aaaacctttc
     gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     cgccgtcaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     gcaattaatg tgagttagcg cgaattgatc tg
//