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ID   PTRXNM     preliminary; circular DNA; SYN; 2873 BP.
XX
AC   IG1134;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pTRXN- - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTRXN- from pBR322
RA   ;
RT   ;
RL   Unpublished (1992).
XX
CC   + makes non-coding strand of gene. - makes coding strand of gene.
CC   NM (pTRXN-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (USB)
CC   HO (E.coli)
CC   CP ()
CC   FN (translation)
CC   SE ()
CC   PA (pBR322)
CC   BR (pTRXN+)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   -> pTRXN- 2873bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2873 BP; 702 A; 724 C; 715 G; 732 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
     aatacgactc actataggga aagcttgcat gcctgcacca ggggggtggc tctagctaga
     ggatccccgg gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga
     aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     atttaacgcg aattttaaca aaatattaac gtttacaatt tcaggtggca cttttcgggg
     aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//