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ID   PTT1       preliminary; circular DNA; SYN; 5025 BP.
XX
AC   IG9906;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pTT1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTT1 from pKK233-2
RC   pTT3 from pTT1 & pUCP18
RC   pTT3deltaS from pTT3
RC   pTT4 from pTT3deltaS
RC   pPZ1 from pNM480 & pUCP19
RC   pPZ2 from pNM481 & pUCP19
RC   pPZ3 from pNM482 & pUCP19
RC   pPZ10 from pNM480 & pTT4
RC   pPZ20 from pNM481 & pTT4
RC   pPZ30 from pNM482 & pTT4
RC   pRZ1 from pDF202 & pPZ3
RA   Schweizer H.P.;
RT   "Improved broad-host-range lac-based plasmid vectors for the
RT   isolation and characterization of protein fusions in Pseudomonas
RT   aeruginosa";
RL   Gene 103:87-92(1991).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTT1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKK233-2)(pUCP18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2 SspI-HindIII 501bp 3801..4302,
FT                   \ rrnB 5S/rrnB T1T2 [502bp]
FT                   2. pUCP18 remove HindIII-SmaI 37bp 2271..2308, 4520bp
FT                   -> pTT1 5021bp"
FT   -               1..2270
FT                   /note="pUCP18 1..2270 2270bp
FT                   HindIII = A^AGCTT"
FT   -               2271..2775
FT                   /note="pKK233-2 3801..4305 505bp complement
FT                   SspI =        AAT^ATT
FT                   SmaI = XmaI = CCC^GGG"
FT   -               2776..5025
FT                   /note="pUCP18 2308..4557 2250bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   CDS             0..0
FT                   /note="GEN E. coli rrnB gene"
FT   terminator      0..0
FT                   /note="GEN E. coli rrnB gene T1T2"
FT   misc_binding    0..0
FT                   /note="MCS NsiI-SstI-EcoRI-SmaI-BamHI-SalI-PstI-
FT                   HindIII"
FT   CDS             0..0
FT                   /note="GEN E. coli lacZ gene"
FT   misc_binding    0..0
FT                   /note="SIT EcoRV"
FT   CDS             0..0
FT                   /note="GEN E. coli lacY gene"
XX
SQ   Sequence 5025 BP; 1239 A; 1259 C; 1344 G; 1183 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc
     aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtggat ctcgcggtat
     cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     cccttaacgt gagttttcgt tccactgagc gtcagacccc aattacacgc cactggctgt
     gcttgctggg gtgacggtgg caacggtggc ggccttgctg ggctatcgcg ttggaaagaa
     acgagggaaa ggggactgat aaaccggtct tagcccctcc ccttggtgtc caaccgctct
     gtaggcctct caggcgccgc tggtgccgct ggttggacgc caagggtgaa tccgcctcga
     taccctgatt actcgcttcc tgcgccctct caggcggcga taggggactg gtaaaacggg
     gattgcccag acgcctcccc cgccccttca ggggcacaaa tgcggcccca acggggccac
     gtagtggtgc gttttttgcg tttccaccct tttcttcctt ttccctttta aaccttttag
     gacgtctaca ggccacgtaa tccgtggcct gtagagttta aaaagggacg gatttgttgc
     cattaaggga cggatttgtt gttaagaagg gacggatttg ttgttgtaaa gggacggatt
     tgttgtattg tgggacgcag atacagtgtc cccttataca caaggaatgt cgaacgtggc
     ctcaccccca atggtttaca aaagcaatgc cctggtcgag gccgcgtatc gcctcagtgt
     tcaggaacag cggatcgttc tggcctgtat tagccaggtg aagaggagcg agcctgtcac
     cgatgaagtg atgtattcag tgacggcgga ggacatagcg acgatggcgg gtgtccctat
     cgaatcttcc tacaaccagc tcaaagaagc ggccctgcgc ctgaaacggc gggaagtccg
     gttaacccaa gagcccaatg gcaaggggaa aagaccgagt gtgatgatta ccggctgggt
     gcaaacaatc atctaccggg agggtgaggg ccgtgtagaa ctcaggttca ccaaagacat
     gctgccgtac ctgacggaac tcaccaaaca gttcaccaaa tacgccttgg ctgacgtggc
     caagatggac agcacccacg cgatcaggct ttacgagctg ctcatgcaat gggacagcat
     cggccagcgc gaaatagaaa ttgaccagct gcgaaagtgg tttcaactgg aaggccggta
     tccctcgatc aaggacttca agttgcgagt gcttgatcca gccgtgacgc agatcaacga
     gcacagcccg ctacaggtgg agtgggcgca gcgaaagacc gggcgcaagg tcacacatct
     gttgttcagt tttggaccga agaagcccgc caaggcggtg ggtaaggccc cagcgaagcg
     caaggccggg aagatttcag atgctgagat cgcgaaacag gctcgccctg gtgagacatg
     ggaagcggcc cgcgctcgac taacccagat gccgctggat ctggcctaga ggccgtggcc
     accacggccc ggcctgcctt tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttggctg
     ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
     tctgataaaa cagtttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     aaatgcttca ataatgggta ccgagctcga attcgtaatc atggtcatag ctgtttcctg
     tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//