back Return to this vector's summary.
ID   PTUGA      preliminary; circular DNA; SYN; 4232 BP.
XX
AC   L24193;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTugA - incomplete, bla & repressor.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4232
RC   pTugA, pTugAS from pUC series & T7
RA   Graham R.W.;
RT   "pTugA and pTugAS : Vectors for the high level expression of cloned
RT   genes in E. coli";
RL   Unpublished (1993).
XX
CC   NCBI gi: 454855
CC   NM (pTugA)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli JM105)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC)(T7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp, ori/amp gene
FT                   2. E. coli, tac promoter
FT                   3. T7, gene 10 enhancer
FT                   4. oligo, human factor Xa site
FT                   5. E. coli, lacIq gene
FT                   -> pTugA 4232bp"
FT   promoter        193..220
FT                   /note="E. coli tac promoter"
FT   enhancer        284..323
FT                   /note="PRO phage T7 gene10 translational enhancer"
FT   misc_binding    324..385
FT                   /note="MCS"
FT   misc_binding    817..828
FT                   /note="SIT human factor Xa cleavage site"
FT   CDS             902..1762
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   rep_origin      1827..2522
FT                   /note="ORI pUC18"
FT   CDS             3111..4193
FT                   /note="GEN E. coli lacIq repressor gene;
FT                   NCBI gi: 454857"
XX
SQ   Sequence 4232 BP; 1033 A; 1063 C; 1123 G; 1013 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
     aacaatttca cacaggaaac agaattgatc cataactaac taatctagaa ataattttgt
     ttaactttaa gaaggagata tatccatgga attcgagctc ggtacccggg gatcctctag
     agtcgacctg caggcatgca agcttctgtt ttggcggatg agagaagatt ttcagcctga
     tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc ccgagggtgg
     cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg
     gatggccttt ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat
     gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     gctgtacgca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagca gatcaattcg
     cgcgcgaagg cgaagcggca tgcatttacg ttgacaccat cgaatggtgc aaaacctttc
     gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
     aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
     ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
     cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     gcaattaatg tgagttagcg cgaattgatc tg
//