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ID   PTUGAS     preliminary; circular DNA; SYN; 4735 BP.
XX
AC   L24367;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pTugAS - incomplete, bla & repressor.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4735
RC   pTugA, pTugAS from pUC series & T7
RA   Graham R.W.;
RT   "pTugA and pTugAS : Vectors for the high level expression of cloned
RT   genes in E. coli";
RL   Unpublished (1993).
XX
CC   NCBI gi: 454852
CC   NM (pTugAS)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli JM105)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC)(T7)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp, ori/amp gene
FT                   2. E. coli, tac promoter
FT                   3. T7, gene 10 enhancer
FT                   4. Cellulomonas fimi, cenA gene
FT                   5. oligo, human factor Xa site
FT                   6. E. coli, lacIq gene
FT                   -> pTugAS 4735bp"
FT   enhancer        284..323
FT                   /note="PRO phage T7 gene10 translational enhancer"
FT   misc_binding    325..816
FT                   /note="Cellulomonas fimi endoglucanase A (cenA)
FT                   leader sequence and cellulose binding domain"
FT   promoter        696..723
FT                   /note="E. coli tac promoter"
FT   misc_binding    817..828
FT                   /note="SIT human factor Xa cleavage site"
FT   misc_binding    832..888
FT                   /note="MCS"
FT   CDS             1405..2265
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT                   /gene="lacIq"
FT   rep_origin      2330..3025
FT                   /note="ORI pUC"
FT   CDS             3614..4696
FT                   /note="GEN E. coli lacIq repressor gene;
FT                   NCBI gi: 454854"
XX
SQ   Sequence 4735 BP; 1108 A; 1287 C; 1274 G; 1066 T; 0 other;
     gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
     ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
     gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
     tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
     aacaatttca cacaggaaac agaattgatc cataactaac taatctagaa ataattttgt
     ttaactttaa gaaggagctc cttgatgtcc acccgcagaa ccgccgcagc gctgctggcg
     gccgcggccg tcgccgtcgg cggtctgacc gccctcacca ccaccgccgc gcaggcggct
     cccggctgcc gcgtcgacta cgccgtcacc aaccagtggc ccggcggctt cggcgccaac
     gtcacgatca ccaacctcgg cgaccccgtc tcgtcgtgga agctcgactg gacctacacc
     gcaggccagc ggatccagca gctgtggaac ggcaccgcgt cgaccaacgg cggccaggtc
     tccgtcacca gcctgccctg gaacggcagc atcccgaccg gcggcacggc gtcgttcggg
     ttcaacggct cgtgggccgg gtccaacccg acgccggcgt cgttctcgct caacggcacc
     acctgcacgg gcaccgtgcc gacgaccagc cccacgccga ccccgacgcc gacgaccccc
     acgccgacgc cgaccccgac ccccaccccc acgccgatcg agggcaggcc tgaattcgag
     ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttct gttttggcgg
     atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
     acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
     agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     cgaagcaacg gccccgaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
     tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     aacgacgagc gtgacaccac gatgctgtac gcaatggcaa caacgttgcg caaactatta
     actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcattt acgttgacac
     catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
     cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gcgcgaattg atctg
//