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ID PTUGAS preliminary; circular DNA; SYN; 4735 BP.
XX
AC L24367;
XX
DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pTugAS - incomplete, bla & repressor.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-4735
RC pTugA, pTugAS from pUC series & T7
RA Graham R.W.;
RT "pTugA and pTugAS : Vectors for the high level expression of cloned
RT genes in E. coli";
RL Unpublished (1993).
XX
CC NCBI gi: 454852
CC NM (pTugAS)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli JM105)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUC)(T7)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 2686bp, ori/amp gene
FT 2. E. coli, tac promoter
FT 3. T7, gene 10 enhancer
FT 4. Cellulomonas fimi, cenA gene
FT 5. oligo, human factor Xa site
FT 6. E. coli, lacIq gene
FT -> pTugAS 4735bp"
FT enhancer 284..323
FT /note="PRO phage T7 gene10 translational enhancer"
FT misc_binding 325..816
FT /note="Cellulomonas fimi endoglucanase A (cenA)
FT leader sequence and cellulose binding domain"
FT promoter 696..723
FT /note="E. coli tac promoter"
FT misc_binding 817..828
FT /note="SIT human factor Xa cleavage site"
FT misc_binding 832..888
FT /note="MCS"
FT CDS 1405..2265
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (amp/apr)"
FT /gene="lacIq"
FT rep_origin 2330..3025
FT /note="ORI pUC"
FT CDS 3614..4696
FT /note="GEN E. coli lacIq repressor gene;
FT NCBI gi: 454854"
XX
SQ Sequence 4735 BP; 1108 A; 1287 C; 1274 G; 1066 T; 0 other;
gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
aacaatttca cacaggaaac agaattgatc cataactaac taatctagaa ataattttgt
ttaactttaa gaaggagctc cttgatgtcc acccgcagaa ccgccgcagc gctgctggcg
gccgcggccg tcgccgtcgg cggtctgacc gccctcacca ccaccgccgc gcaggcggct
cccggctgcc gcgtcgacta cgccgtcacc aaccagtggc ccggcggctt cggcgccaac
gtcacgatca ccaacctcgg cgaccccgtc tcgtcgtgga agctcgactg gacctacacc
gcaggccagc ggatccagca gctgtggaac ggcaccgcgt cgaccaacgg cggccaggtc
tccgtcacca gcctgccctg gaacggcagc atcccgaccg gcggcacggc gtcgttcggg
ttcaacggct cgtgggccgg gtccaacccg acgccggcgt cgttctcgct caacggcacc
acctgcacgg gcaccgtgcc gacgaccagc cccacgccga ccccgacgcc gacgaccccc
acgccgacgc cgaccccgac ccccaccccc acgccgatcg agggcaggcc tgaattcgag
ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttct gttttggcgg
atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa
acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga
agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg
ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
cgaagcaacg gccccgaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta
tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
aacgacgagc gtgacaccac gatgctgtac gcaatggcaa caacgttgcg caaactatta
actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcattt acgttgacac
catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gcgcgaattg atctg
//