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ID   PTZ12      preliminary; circular DNA; SYN; 2517 BP.
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AC   M16192;
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DT   23-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus plasmid vector pTZ12 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2517
RC   pTZ10 from Cornyebacterium xerosis
RC   pTZ11 from pTZ10 & pT181 & pC221
RC   pTZ12 from pTZ11
RA   Aoki T., Noguchi N., Sasatsu M., Kono M.;
RT   "Complete nucleotide sequence of pTZ12, a chloramphenicol-
RT   resistance plasmid of Bacillus subtilis";
RL   Gene 51:107-111(1987).
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RN   [2]
RC   pTZ12
RA   Kono M.;
RT   ;
RL   Submitted (23-JUN-1987) to GenBank on computer-readable media by:
RL   Kono M., .
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CC   The Rep protein of pTZ12 appears to be a
CC   fusion protein of pT181 RepC and pC221 RepD proteins, formed
CC   spontaneously by a recombination event between a pT181-like plasmid
CC   and a pC221-like plasmid.
CC   NM (pTZ12)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (B.subtilis)(Corynebacterium xerosis)(Bacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pT181)(pC221)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Cornyebacterium xerosis
FT                   -> pTZ10
FT                   1. Staphylococcus, cat gene
FT                   -> pC221
FT                   1. pTZ10
FT                   2. pT181 4437bp, tet gene
FT                   3. pC221, cat gene
FT                   -> pTZ11 7200bp
FT                   1. pTZ11 XbaI-XbaI, XbaI-B fragment/pT181 XbaI = 652
FT                   \ 3100bp
FT                   300bp deleted:300bp deleted, 2517bp
FT                   -> pTZ12 2517bp"
FT   CDS             119..1063
FT                   /note="GEN B. subtilis Rep gene,
FT                   a fusion of pT181 RepC and pC221 RepD genes"
FT   CDS             1193..1840
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
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SQ   Sequence 2517 BP; 968 A; 302 C; 442 G; 805 T; 0 other;
     tgagttgttt ttataatctt gtatatttag atattaaacg atatttaaat atacatcaag
     atatatattt gggtgagcga ttccttaaac gaaattgaga ttaaggagtc gattttttat
     gtataaaaac aatcatgcaa atcattcaaa tcatttggaa aatcacgatt tagacaattt
     ttctaaaacc ggctactcta atagccggtt aagtggtaat ttttttacca cccctcaacc
     agaattaagt tttgatgcta tgacaatcgt tgggaatttg aacaaaacta acgctaaaaa
     gctatctgat tttatgagta cagagccaca aataaggctt tgggatatat tacaaacaaa
     gtttaaagct aaagcacttc aagaaaaggt ttatatcgaa tatgacaaag taaaagcaga
     tagttgggat agacgtaata tgcgtgttga atttaatcca aataaactca cacatgaaga
     aatgctttgg ttaaaacaaa atattatcga ctacatggaa gatgacggtt ttacgaggtt
     agatttagct tttgattttg aagatgattt gagtgattac tacgcaatga ctgataaagc
     agttaagaaa actatttttt atggtcgtaa tggtaagcca gaaacaaaat attttggtgt
     tcgtgacagt gatagattta ttagaattta taataaaaaa caagaacgta aagataacgc
     cgatgttgaa gttatgtctg aacatttatg gcgtgtagaa attgaattga aaagagatat
     ggttgattac tggaatgatt gctttgatga tttacacatt ttgaaacccg attggacaac
     accagaaaaa gtaaaagaac aagcaatggt ttatttgtta ctgaatgaag aaggcacgtg
     gggaaaactt gaaagacatg ctaaatataa atacaagcaa ttgattaaag aaatatctcc
     aattgattta acggaattaa tgaaatcgac tttaaaagag aatgaaaagc aattgcaaaa
     acagattgat ttttggcaac gtgaatttag attttggaag taaaataagt tttattttat
     aaaaattgct gattcagtat aattaatatt tacggggtga cataacgtat gaaaaaatca
     gaggattatt cctcctaaat ataaagattt aaaatttagg aggaaattat atatgacttt
     taatattatc aaattagaaa attgggatag aaaagaatat tttgaacact attttaacca
     gcaaactacg tatagcatta ctaaagaaat tgatattact ttgtttaaag atatgataaa
     aaagaaagga tatgaaattt atccttcttt gatttatgca attatggaag ttgtaaataa
     aaataaagtg tttagaacag gaattaatag tgagaataaa ttaggttatt gggataagtt
     aaatcctttg tatacagttt ttaataagca aactgaaaaa tttactaaca tttggactga
     atctgataac aacttcactt ctttttataa taattataaa aatgacttgc ttgaatataa
     agataaagaa gaaatgtttc ctaaaaaacc gatacctgaa aacaccatac cgatttcaat
     gattccttgg attgatttta gttcatttaa tttaaacatt ggtaacaata gcaacttttt
     attgcctatt attacgatag gtaaatttta tagtgagaat aataaaattt atataccagt
     tgctttgcag cttcatcatg ctgtatgtga tggttaccat gcttcattat ttatgaatga
     atttcaagat ataattcata aggtagatga ttggatttag tttttagatt ttggaagtga
     atttaatttt atacacgcaa gtgatcataa aatttatgaa cgtatagcaa ccacattttt
     ggttgcatag gttttgattt tgaattaggt cttgaactat gagtggctag cactttgcca
     ctcatttttt gcgttagcaa aaacataaag ggtatgggat ataatcccat caagccggta
     tattcagaac aggaacagca acaagaacaa caaaagaatc aaaaacgaga tagaggtatg
     cacttataga acatgcattt atgccgagaa aacttattgg ttggaatggg ctatgtgtta
     gctaacttgt tagcgagttg gttggacttg aattgggatt aatcccaaga aagtaccaac
     tcaacaacac ataaagccct gtaggttccg accaataagg aaattggaat aaagcaataa
     aaggagttga agaaatgaaa ttcagagaag cctttgagaa ttttataaca agtaagtatg
     tacttggtgt tttagtagtt ttaactgttt accagataat acaaatgctt aaataaaaaa
     agacttgatc tgattagacc aagtcttttg atagtgttat attaataaca aaataaaaag
     gagtcgctca cgccttgacc aaagtttgtg aacgacatca ttcaaagaaa aaaacac
//