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ID   PTZSV28    preliminary; circular DNA; SYN; 5871 BP.
XX
AC   IG7048;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pTZSV28 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pTZSV12, pTZSV22, pTZSV28 from pTZ19R & SV40 early region
RC   Inter-HN2-pTZSV28 from pTZSV28 & oligo
RC   C-pTZSV28 from pTZSV28
RA   Grueneberg D.A., Ojwang J.O., Benasutti M., Hartman S., Loechler E.L.;
RT   "Construction of a human shuttle vector containing a single
RT   nitrogen mustard interstrand, DNA-DNA cross-link at a unique
RT   plasmid location";
RL   Cancer Res. 51:2268-2272(1991).
XX
RN   [2]
RC   pTZSV28 from pTZ19R & SV40
RA   Basu A.K., Essigmann J.M.;
RT   "Site-specificaly modified oligodeoynucleotides as probes for the
RT   structural and biological effects of DNA-damaging agents";
RL   Chem. Res. Toxicol. 1:1-18(1988).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pTZSV28)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli MM294A)(human 293 cells)
CC   CP ()
CC   FN (cross-linking)
CC   SE (color blue/white)
CC   PA (pTZ19R)(SV40)
CC   BR (pTZSV12)(pTZSV22)
CC   OF (Inter-HN2-pTZSV28)(C-pTZSV28)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pTZ19R SspI 2863bp 2841..2841
FT                   2. SV40 BamHI-KpnI 3008bp 2534..5243..299,
FT                   \ early region
FT                   blunt end:blunt end
FT                   -> pTZSV28 5879bp
FT                   \ [mutated PstI in large T; MluI in MCS]"
FT   -               1..2840
FT                   /note="pTZ19R 1..2840 2840bp
FT                   SspI = AAT^ATT
FT                   BamHI =  G^GATCC"
FT   -               2841..5848
FT                   /note="SV40 2534..5243..298 3008bp
FT                   KpnI = GGTAC^C
FT                   SspI =   AAT^ATT"
FT   -               5849..5871
FT                   /note="pTZ19R 2841..2863 23bp"
FT   promoter        0..0
FT                   /note="PRO SV40 enhancer"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   misc_feature    0..0
FT                   /note="GEN SV40 small t antigen"
FT   misc_feature    0..0
FT                   /note="GEN SV40 large T antigen"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    0..0
FT                   /note="SIT unique NaeI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique AhaII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5871 BP; 1567 A; 1401 C; 1206 G; 1697 T; 0 other;
     cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     gatcctctag agtcgacctg caggcatgca agctttccct atagtgagtc gtattagagc
     ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc tttagggttc
     cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     aaatttaacg cgaattttaa gatccagaca tgataagata cattgatgag tttggacaaa
     ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     gtggtatggc tgattatgat catgaacaga ctgtgaggac tgaggggcct gaaatgagcc
     ttgggactgt gaatcaatgc ctgtttcatg ccctgagtct tccatgttct tctccccacc
     atcttcattt ttatcagcat tttcctggct gtcttcatca tcatcatcac tgtttcttag
     ccaatctaaa actccaattc ccatagccac attaaacttc attttttgat acactgacaa
     actaaactct ttgtccaatc tctctttcca ctccacaatt ctgctctgaa tactttgagc
     aaactcagcc acaggtctgt accaaattaa cataagaagc aaagcaatgc cactttgaat
     tattctcttt tctaacaaaa actcactgcg ttccaggcaa tgctttaaat aatctttggg
     cctaaaatct atttgtttta caaatctggc ctgcagtgtt ttaggcacac tgtactcatt
     catggtgact attccagggg gaaatatttg agttctttta tttaggtgtt tcttttctaa
     gtttacctta acactgccat ccaaataatc ccttaaattg tccaggttat taattccctg
     acctgaaggc aaatctctgg actcccctcc agtgcccttt acatcctcaa aaactactaa
     aaactggtca atagctactc ctagctcaaa gttcagcctg tccaagggca aattaacatt
     taaagctttc cccccacata attcaagcaa agcagctgct aatgtagttt taccactatc
     aattggtcct ttaaacagcc agtatctttt tttaggaatg ttgtacacca tgcattttaa
     aaagtcatac accactgaat ccattttggg caacaaacag tgtagccaag caactccagc
     catccattct tctatgtcag cagagcctgt agaaccaaac attatatcca tcctatccaa
     aagatcatta aatctgtttg ttaacatttg ttctctagtt aattgtaggc tatcaacccg
     ctttttagct aaaacagtat caacagcctg ttggcatatg gttttttggt ttttgctgtc
     agcaaatata gcagcatttg cataatgctt ttcatggtac ttatagtggc tgggctgttc
     ttttttaata cattttaaac acatttcaaa actgtactga aattccaagt acatcccaag
     caataacaac acatcatcac attttgtttc cattgcatac tctgttacaa gcttccagga
     cacttgttta gtttcctctg cttcttctgg attaaaatca tgctccttta acccacctgg
     caaactttcc tcaataacag aaaatggatc tctagtcaag gcactataca tcaaatattc
     cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     ttatgtcaca ccacagaagt aaggttcctt cacaaagatc aagtccaaac cacattctaa
     agcaatcgaa gcagtagcaa tcaacccaca caagtggatc tttcctgtat aattttctat
     tttcatgctt catcctcagt aagcacagca agcatatgca gttagcagac attttctttg
     cacactcagg ccattgtttg cagtacattg catcaacacc aggatttaag gaagaagcaa
     atacctcagt tgcatcccag aagcctccaa agtcaggttg atgagcatat tttactccat
     cttccatttt cttgtacaga gtattcattt tcttcatttt ttcttcatct cctcctttat
     caggatgaaa ctccttgcat ttttttaaat atgcctttct catcagagga atattccccc
     aggcactcct ttcaagacct agaaggtcca ttagctgcaa agattcctct ctgtttaaaa
     ctttatccat ctttgcaaag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     agctggttct ttccgcctca gaaggtacca aaatattaac gtttacaatt t
//