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ID   PUB110     preliminary; circular DNA; PRO; 4548 BP.
XX
AC   X03885; M19465; IG0107; ATCC37015;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pUB110 - complete.
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KW   cloning vector.
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OS   Staphylococcus aureus
OC   Prokaryota; Bacteria; Firmicutes; Gram-positive cocci;
OC   Micrococcaceae.
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RN   [1]
RP   1-4548
RC   pUB110 from Bacillus
RA   McKenzie T., Hoshino T., Tanaka T., Sueoka N.;
RT   "The Nucleotide Sequence of pUB110:  Some Salient Features in
RT   Relation to Replication and Its Regulation";
RL   Plasmid 15:93-103(1986).
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RN   [2]
RC   pRB165 from pUB110 & pUR250
RC   pZY165, pZY166, pZY167 from pRB165 & pUB110
RC   pZY165-lacZ from pZY165 & pMC1981
RC   pZY165/CS1826 from pZY165 & pUB112, cat gene
RA   Zyprian E., Matzura H.;
RT   "Characterization of signals promoting gene expression on the
RT   Staphylococcus aureus plasmid pUB110 and development of a
RT   gram-positive expression vector system";
RL   DNA 5:219-225(1986).
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RN   [3]
RP   1-4547
RC   pUB110
RA   Sueoka N.;
RT   ;
RL   Submitted (13-OCT-1986) to the EMBL Data Library by:
RL   Sueoka N., .
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RN   [4]
RC   pUB101 from Bacillus pen gene/cad gene/fus gene
RC   pUB113 from pUB101
RC   pUB117 from pUB113
RA   Chopra I.;
RT   "Mechanisms of fusidic acid resistance in Staphylococcus aureus";
RL   J. Gen. Microbiol. 96:229-238(1976).
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RN   [5]
RC   pUB108 from Staphylococcus, strep gene
RC   pUB109 from Staphylococcus, strep gene
RC   pUB111 from Staphylococcus, tet gene
RC   pUB112 from Staphylococcus, cat gene
RA   Chopra I., Bennett P.M., Lacey R.W.;
RT   "A variety of staphylococcal plasmids present as multiple copies";
RL   J. Gen. Microbiol. 79:343-345(1973).
XX
RN   [6]
RC   pPL576, pPL10, pPL7065 from B.pumilis
RC   pPL2 from B.subtilis
RC   pSL103, pSL104 from pUB110 & B.pumilis trpCF gene
RC   pSL101, pSL105 from pUB110 & B.licheniformis trpCF gene
RC   pSL106 from pUB110 & B.subtilis trpCF gene
RA   Keggins K., Lovett P., Duvall D.;
RT   "Molecular cloning of genetically active fragments of Bacillus DNA
RT   in Bacillus subtilis and properties of the vector plasmid pUB110";
RL   Proc. Natl. Acad. Sci. U.S.A. 75:1423-1427(1978).
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RN   [7]
RC   pLB1 from pUB110 & pC194
RC   pLB2 from pLB1
RC   pLB4 from pBR322 & pLB2
RC   pLB5 from pLB4
RC   pLB2-1S, pLB2-3S from pLB2 & B.subtilis DNA
RC   pLB5-1S, pLB5-3S from pLB5 & B.subtilis DNA
RC   pLB2-1C, pLB2-3C from pLB2 & E.coli DNA
RC   pLB5-1C, pLB5-3C from pLB5 & E.coli DNA
RA   Bron S., Luxen E.;
RT   "Segregational instability of pUB110-derived recombinant plasmids
RT   in Bacillus subtilis";
RL   Plasmid 14:235-244(1985).
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RN   [8]
RC   pEB1 from pUB110 & pE194
RC   pEB3 from pEB1
RC   pEB4 from pEB1
RC   pEB5 from pEB1
RC   pEB10 from pUB110 & pBR322
RC   pEB11 from pHV60 & pUB110
RA   Bron S., Luxen E., Swart G.;
RT   "Instability of recombinant pUB110 plasmids in Bacillus subtilis:
RT   plasmid-encoded stability function and effects of DNA inserts";
RL   Plasmid 19:231-241(1988).
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RN   [9]
RC   from pBD9
RC   from pBD15
RC   from pUB110
RC   from pSA2100
RC   from pC194
RA   Contente S., Dubnau D.;
RT   "Characterization of plasmid transformation in Bacillus subtilis:
RT   kinetic properties and the effect of DNA conformation";
RL   Mol. Gen. Genet. 167:251-258(1979).
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CC   Staphylococcus aureus plasmid pUB110 replicates in B. subtilis.
CC   Transfer between Bacillus species by transformation or
CC   transduction using bacteriophages PBS1, AR9, or Sp10.
CC   A natural isolate general purpose plasmid vector for cloning in
CC   Bacillus. [4]
CC   Medium is 1179 V Y medium.
CC   NM (pUB110)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Sigma)(ATCC)
CC   HO (Staphylococcus aureus)(B.subtilis BD170)(B.subtilis)
CC   CP (30-50 copies/cell)
CC   FN (regulation of replication initiation in B.subtilis)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus aureus
FT                   -> pUB110 4548bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI"
FT   CDS             complement(1..933)
FT                   /note="GEN S. aureus ORF beta (aa 1-311)"
FT   CDS             complement(1..1074)
FT                   /note="GEN S. aureus ORF beta (aa 1-362)"
FT   CDS             complement(1582..1980)
FT                   /note="GEN S. aureus open reading frame gamma"
FT   CDS             complement(2205..2963)
FT                   /note="ANT S. aureus neomycin phosphotransferase gene
FT                   (NPT), neomycin resistance gene (neo)
FT                   kanamycin resistance gene (kan)"
FT   misc_signal     complement(3106..3131)
FT                   /note="TER S. aureus pot. transcription terminator
FT                   (alpha)"
FT   misc_binding    complement(3795..3854)
FT                   /note="SIT consensus sequence for DNA binding site"
FT   CDS             complement(3135..4136)
FT                   /note="GEN S. aureus put. protein alpha (aa 1-334)
FT                   (may be replication gene - rep)"
FT   misc_signal     complement(4326..4356)
FT                   /note="TER S. aureus pot. transcription terminator
FT                   (beta)"
FT   CDS             complement(4361..4548)
FT                   /note="GEN S. aureus ORF beta (aa 363-424)"
FT   CDS             complement(0..0)
FT                   /note="GEN S. aureus delta gene"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII"
FT   rep_origin      complement(0..0)
FT                   /note="ORI Staphylococcus aureus"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII"
FT   misc_binding    0..0
FT                   /note="SIT unique TacI"
XX
SQ   Sequence 4548 BP; 1307 A; 1000 C; 624 G; 1617 T; 0 other;
     gaattcctta gtgctttcat agattaaact cacatcacgc tttaaatcgc ttattttaga
     ctttaaagac ttgttttctt caagcaactc attataatca tttacatttt cattaaatcg
     ctctacaaga ccactatatt tttctttaac ttgcccatgt tctttactta attttttata
     ttctctcgcc atatcagtac tcatgagatt tctaacatgc tgttttaacc tatcgttatc
     tctcgcagca gtcactaagt ttttataatc acgctccgat ataacaacat ttttggttgg
     tttcttttct gttttcatta tttcttttcc caaaccaaac atggactttt cacccgttgg
     cacttcaaca cttttcatgt gtcgtttcgc tggtacttct aaatctgatt taactttatc
     gctataagca gtccattcat cttttttaac tgctaaattt ttttctagaa aatcaatctc
     tttttccaaa gtttgttttt taaatttagc tgtctcaata tgtttacggt cagagccacg
     ttcaccacgc ttcaactcaa aaccctgttt tttcatatgc tcggggaatt tatcttgtag
     ccataacagt tcttgacgat taaacacatt ttttccttgc agttttccat cacgcatagg
     cacaacacct aaatgcatgt gaggggtttg ctcatcatta tgaactgttg cataagcaat
     attttgcttg ccatatcgtt cggaaaataa tttataactt tcctcaaaaa atcgtttttg
     ttctcctgga tccagttgct caaaaaaatc tcggtcagat gttactagca actcatttac
     aagaacagca tctttcctcc gtttttcttg tacctgtttt ttgtgattca ataatttctt
     tgacacgttc gttgtaatca atatttttat catttttcaa atcataattt tcacgtgttc
     gctcatggtc aatatcatca ttcgttctac tttttcgctc tctttgatta tgaaattgca
     tgccttttag tccagctgat ttcacttttt gcattctaca aactgcataa ctcatatgta
     aatcgctcct ttttaggtgg cacaaatgtg aggcattttc gctctttccg gcaaccactt
     ccaagtaaag tataacacac tatactttat attcataaag tgtgtgctct gcgaggctgt
     cggcagtgcc gaccaaaacc ataaaacctt taagaccttt ctttttttta cgagaaaaaa
     gaaacaaaaa aacctgccct ctgccacctc agcaaagggg ggttttgctc tcgtgctcgt
     ttaaaaatca gcaagggaca ggtagtattt tttgagaaga tcactcaaaa aatctccacc
     tttaaaccct tgccaatttt tattttgtcc gttttgtcta gcttaccgaa agccagactc
     agcaagaata aaatttttat tgtctttcgg ttttctagtg taacggacaa aaccactcaa
     aataaaaaag atacaagaga ggtctctcgt atcttttatt cagcaatcgc gcccgattgc
     tgaacagatt aataatagat tttagctttt tatttgttga aaaaagctaa tcaaattgtt
     gtcgggatca attactgcaa agtctcgttc atcccaccac tgatctttta atgatgtatt
     ggggtgcaaa atgcccaaag gcttaatatg ttgatataat tcatcaattc cctctacttc
     aatgcggcaa ctagcagtac cagcaataaa cgactccgca cctgtacaaa ccggtgaatc
     attactacga gagcgccagc ttcatcactt gcctcccata gatgaatccg aacctcatta
     cacattagaa ctgcgaatcc atcttcatgg tgaaccaaag tgaaacctag tttatcgcaa
     taaaaaccta tactcttttt aatatccccg actggcaatg ccgggataga ctgtaacatt
     ctcacgcata aaatcccctt tcattttcta atgtaaatct attaccttat tattaattca
     attcgctcat aattaatcct ttttcttatt acgcaaaatg gcccgattta agcacaccct
     ttattccgtt aatgcgccat gacagccatg ataattacta atactaggag aagttaataa
     atacgtaacc aacatgatta acaattatta gaggtcatcg ttcaaaatgg tatgcgtttt
     gacacatcca ctatatatcc gtgtcgttct gtccactcct gaatcccatt ccagaaattc
     tctagcgatt ccagaagttt ctcagagtcg gaaagttgac cagacattac gaactggcac
     agatggtcat aacctgaagg aagatctgat tgcttaactg cttcagttaa gaccgaagcg
     ctcgtcgtat aacagatgcg atgatgcaga ccaatcaaca tggcacctgc cattgctacc
     tgtacagtca aggatggtag aaatgttgtc ggtccttgca cacgaatatt acgccatttg
     cctgcatatt caaacagctc ttctacgata agggcacaaa tcgcatcgtg gaacgtttgg
     gcttctaccg atttagcagt ttgatacact ttctctaagt atccacctga atcataaatc
     ggcaaaatag agaaaaattg accatgtgta agcggccaat ctgattccac ctgagatgca
     taatctagta gaatctcttc gctatcaaaa ttcacttcca ccttccactc accggttgtc
     cattcatggc tgaactctgc ttcctctgtt gacatgacac acatcatctc aatatccgaa
     tagggcccat cagtctgacg accaagagag ccataaacac caatagcctt aacatcatcc
     ccatatttat ccaatattcg ttccttaatt tcatgaacaa tcttcattct ttcttctcta
     gtcattatta ttggtccatt cactattctc attccctttt cagataattt tagatttgct
     tttctaaata agaatatttg gagagcaccg ttcttattca gctattaata actcgtcttc
     ctaagcatcc ttcaatcctt ttaataacaa ttatagcatc taatcttcaa caaactggcc
     cgtttgttga actactcttt aataaaataa tttttccgtt cccaattcca cattgcaata
     atagaaaatc catcttcatc ggctttttcg tcatcatctg tatgaatcaa atcgccttct
     tctgtgtcat caaggtttaa ttttttatgt atttctttta acaaaccacc ataggagatt
     aaccttttac ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt cttttcttca
     tcatcggtca taaaatccgt atcctttaca ggatattttg cagtttcgtc aattgccgat
     tgtatatccg atttatattt atttttcggt cgaatcattt gaacttttac atttggatca
     tagtctaatt tcattgcctt tttccaaaat tgaatccatt gtttttgatt cacgtagttt
     tctgtattct taaaataagt tggttccaca cataccaata catgcatgtg ctgattataa
     gaattatctt tattatttat tgtcacttcc gttgcacgca taaaaccaac aagattttta
     ttaatttttt tatattgcat cattcggcga aatccttgag ccatatctga caaactctta
     tttaattctt cgccatcata aacattttta actgttaatg tgagaaacaa ccaacgaact
     gttggctttt gtttaataac ttcagcaaca accttttgtg actgaatgcc atgtttcatt
     gctctcctcc agttgcacat tggacaaagc ctggatttac aaaaccacac tcgatacaac
     tttctttcgc ctgtttcacg attttgttta tactctaata tttcagcaca atcttttact
     ctttcagcct ttttaaattc aagaatatgc agaagttcaa agtaatcaac attagcgatt
     ttcttttctc tccatggtct cacttttcca ctttttgtct tgtccactaa aacccttgat
     ttttcatctg aataaatgct actattagga cacataatat taaaagaaac ccccatctat
     ttagttattt gtttagtcac ttataacttt aacagatggg gtttttctgt gcaaccaatt
     ttaagggttt tcaatacttt aaaacacata cataccaaca cttcaacgca cctttcagca
     actaaaataa aaatgacgtt atttctatat gtatcaagat aagaaagaac aagttcaaaa
     ccatcaaaaa aagacacctt ttcaggtgct ttttttattt tataaactca ttccctgatc
     tcgacttcgt tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg
     ttctaaatcg tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc
     cttaaaaacg tttttaaagg cttttaagcc gtctgtacgt tccttaag
//