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ID   PUC119     preliminary; circular DNA; SYN; 3162 BP.
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AC   U07650; IG1155; ATCC37461;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUC119 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3162
RC   pUC119 from pUC19
RA   Kitts P.A.;
RT   ;
RL   Unpublished (1994).
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RN   [2]
RP   1-3162
RC   pUC119
RA   Kitts P.A.;
RT   ;
RL   Submitted (11-MAR-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
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CC   A pUC derivative containing the IG (intergenic) region of M13 for
CC   production of ssDNA.
CC   Medium is 1227 LB plus ampicillin.
CC   NCBI gi: 464018
CC   NM (pUC119)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (USB)(Worthington)(ATCC)
CC   HO (E.coli JM103)(E.coli JM101)(E.coli JM105)(E.coli)
CC   CP ()
CC   FN (cloning)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pUC19)
CC   BR (pUC118)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp, pMB1 ori/amp gene
FT                   2. M13 480bp, ori
FT                   -> pUC119 3162bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-HincII-AccI-SalI-XbaI-
FT                   BamHI-SmaI-XmaI-Asp718-KpnI-SstI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   misc_binding    51..51
FT                   /note="SIT unique Esp3I"
FT   misc_binding    91..91
FT                   /note="SIT double DrdI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoO109I"
FT   misc_binding    181..181
FT                   /note="SIT unique HgiEII"
FT   misc_binding    183..183
FT                   /note="SIT unique NdeI"
FT   misc_binding    235..235
FT                   /note="SIT unique NarI"
FT   misc_binding    235..235
FT                   /note="SIT triple HaeII (lac fragment)"
FT   CDS             complement(236..469)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     364..386
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     379..395
FT                   /note="PRI bacteriophage M13 forward"
FT   misc_binding    399..399
FT                   /note="SIT unique HindIII"
FT   misc_binding    399..450
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   misc_binding    450..450
FT                   /note="SIT unique EcoRI"
FT   primer_bind     461..476
FT                   /note="PRI bacteriophage T7T3alpha"
FT   CDS             complement(470..591)
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    680..680
FT                   /note="SIT triple HaeII (lac fragment)"
FT   misc_binding    806..806
FT                   /note="SIT unique AflIII"
FT   rep_origin      complement(861..861)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   misc_binding    908..908
FT                   /note="SIT double DrdI"
FT   misc_binding    1217..1217
FT                   /note="SIT unique AlwNI"
FT   misc_binding    1387..1387
FT                   /note="SIT unique HgiEI"
FT   CDS             complement(1626..2486)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1779..1779
FT                   /note="SIT unique Cfr101"
FT   misc_binding    1784..1784
FT                   /note="SIT unique GsuI"
FT   misc_binding    2177..2177
FT                   /note="SIT unique ScaI"
FT   misc_binding    2215..2215
FT                   /note="SIT unique BcgI"
FT   misc_binding    2294..2294
FT                   /note="SIT unique XmnI"
FT   misc_binding    2501..2501
FT                   /note="SIT unique SspI"
FT   misc_binding    2531..2531
FT                   /note="SIT unique BsmAI"
FT   misc_binding    2617..2617
FT                   /note="SIT unique AatII"
FT   misc_binding    2674..2674
FT                   /note="SIT unique DraII"
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SQ   Sequence 3162 BP; 762 A; 812 C; 780 G; 808 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc
     gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//