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ID   PUC13      preliminary; circular DNA; SYN; 2680 BP.
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AC   L09130; A02712; VB0024; IG0109;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pUC13 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-2680
RC   pUC13
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   Expression Vector For Adjustable Expression Of Exogenous
CC   Genes In Prokaryotes; Patent number WO8809373-A/10 (01-DEC-1988).
CC   This patent sequence is correct.
CC
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled from pUC19 and M13mp11 by F. Pfeiffer, MPI, Martinsried
CC   old Pharmacia vector.
CC   NM (pUC13)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli JM103)
CC   CP ()
CC   FN (cloning)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (M13mp7)(pBR322)(pUC9)(M13mp11)
CC   BR (pUC12)
CC   OF (pUC19)(pUC19c)(pSP65)(pGEM-2)(pT713)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC9 remove HindIII-EcoRI 30bp 234..264,
FT                   \ MCS/2635bp
FT                   2. M13mp10 EcoRI-HindIII 45bp 6232..6277, MCS
FT                   -> pUC13 2680bp"
FT   CDS             1..230
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    233..283
FT                   /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-
FT                   EcoRI; polylinker of M13mp11"
FT   CDS             284..441
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322),
FT                   base 1 of origin equals base 2068 of pBR322"
FT   CDS             946..1734
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 2680 BP; 657 A; 683 C; 676 G; 664 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     gctgcaggtc gactctagag gatccccggg cgagctcgaa ttcactggcc gtcgttttac
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//